Label-Free Electrochemical Aptasensor for Sensitive Detection of Malachite Green Based on Au Nanoparticle/Graphene Quantum Dots/Tungsten Disulfide Nanocomposites

Wang, Qianqian; Qin, Xiaofei; Geng, Liping; Wang, Yan

doi:10.3390/nano9020229

Cited by 36 publications

(6 citation statements)

References 41 publications

(37 reference statements)

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“…The development of electrochemical aptasensors without labelling is of interest because there is no need to modify the aptamer sequence. Wang et al developed a label free electrochemical aptasensors for the detection of malachite green [31] (Figure 6). Taghdisi et al developed a label-free electrochemical aptasensor based on laddershaped DNA structure for the detection of ampicillin in milk [32] (Figure 7).…”

Section: Change Of Aptamer Conformationmentioning

confidence: 99%

Contribution of Nanomaterials to the Development of Electrochemical Aptasensors for the Detection of Antimicrobial Residues in Food Products

Gaudin¹

2021

Chemosensors

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The detection of antimicrobial residues in food products of animal origin is of utmost importance. Indeed antimicrobial residues could be present in animal derived food products because of animal treatments for curative purposes or from illegal use. The usual screening methods to detect antimicrobial residues in food are microbiological, immunological or physico-chemical methods. The development of biosensors to propose sensitive, cheap and quick alternatives to classical methods is constantly increasing. Aptasensors are one of the major trends proposed in the literature, in parallel with the development of immunosensors based on antibodies. The characteristics of electrochemical sensors (i.e., low cost, miniaturization, and portable instrumentation) make them very good candidates to develop screening methods for antimicrobial residues in food products. This review will focus on the recent advances in the development of electrochemical aptasensors for the detection of antimicrobial residues in food products. The contribution of nanomaterials to improve the performance characteristics of electrochemical aptasensors (e.g., Sensitivity, easiness, stability) in the last ten years, as well as signal amplification techniques will be highlighted.

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Section: Change Of Aptamer Conformationmentioning

confidence: 99%

Contribution of Nanomaterials to the Development of Electrochemical Aptasensors for the Detection of Antimicrobial Residues in Food Products

Gaudin¹

2021

Chemosensors

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“…Compared with the spectrophotometric method [ 47 ], the present method not only has a lower LOD value but also overcomes the disadvantages such as the requirement of extraction columns for sample preparation. The present LOD is similar to that of the electrochemical method [ 48 ], but the electrochemistry requires complex nanomaterials for electrode preparation. Fluorescence and colorimetric methods based on carbon dot, gold nanoclusters and AuNPs have been developed for MG detection [ 49 – 51 ].…”

Section: Resultsmentioning

confidence: 83%

Fluorescence detection of malachite green in fish based on aptamer and SYBR Green I

Wang¹,

Hong²,

Lin³

et al. 2022

Journal of Food and Drug Analysis

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A simple fluorescence method to quantitatively monitor malachite green (MG) was introduced based on the conformation switch effect of MG-aptamer (Apt) and the fluorochrome of SYBR Green I (SGI). SGI can embed in the stem-loop structure of Apt to produce fluorescence. However, the fluorescence will be quenched when MG is introduced because of the specific binding of Apt to MG. Under the optimal conditions, the fluorescence method displayed excellent linear relationship between the fluorescence quenching efficiencies and the MG concentrations ranging from 0.02 μmol L −1 to 1.0 μmol L −1 . The detection limit of the method was 0.67 μg kg −1 (3 α / κ , n = 9) which is lower than the limit (1 μg kg −1 ) specified in the national standard of China (GB/T 19857–2005) and the limit (2 μg kg −1 ) specified by the European Food Safety Authority (EFSA, 2016). Excellent recoveries between 87.1% and 106.9% tested by spiking standards were achieved with RSD less than 6.4%. The data were confirmed by the method of HPLC, and the results proved that the established fluorescence method could be used for the rapid detection of MG in fish with high accuracy and sensitivity.

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“…Zero-dimensional graphene in the form of so-called graphene quantum dots has been successfully implemented in aptasensor design for the detection of malachite green (MG), as proposed by Wang et al [ 87 ]. The surface of the voltammetric transducer was based on a glassy carbon electrode modified with a Au nanoparticles/graphene quantum dots/tungsten disulfide nanosheet composite film (AuNPs/GQDs-WS 2 /GCE) ( Figure 10 ).…”

Section: Role Of Carbon Nanomaterials and Their Composites In The Des...mentioning

confidence: 99%

“… Procedure for fabrication of aptasensor receptor layer for label-free detection of malachite green. Adapted with permission from Wang et al [ 87 ] (under the terms and conditions of the Creative Commons CC BY License 4.0). …”

Section: Figurementioning

confidence: 99%

Modern Electrochemical Biosensing Based on Nucleic Acids and Carbon Nanomaterials

Szymczyk

Ziółkowski

Malinowska

2023

Sensors

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To meet the requirements of novel therapies, effective treatments should be supported by diagnostic tools characterized by appropriate analytical and working parameters. These are, in particular, fast and reliable responses that are proportional to analyte concentration, with low detection limits, high selectivity, cost-efficient construction, and portability, allowing for the development of point-of-care devices. Biosensors using nucleic acids as receptors has turned out to be an effective approach for meeting the abovementioned requirements. Careful design of the receptor layers will allow them to obtain DNA biosensors that are dedicated to almost any analyte, including ions, low and high molecular weight compounds, nucleic acids, proteins, and even whole cells. The impulse for the application of carbon nanomaterials in electrochemical DNA biosensors is rooted in the possibility to further influence their analytical parameters and adjust them to the chosen analysis. Such nanomaterials enable the lowering of the detection limit, the extension of the biosensor linear response, or the increase in selectivity. This is possible thanks to their high conductivity, large surface-to-area ratio, ease of chemical modification, and introduction of other nanomaterials, such as nanoparticles, into the carbon structures. This review discusses the recent advances on the design and application of carbon nanomaterials in electrochemical DNA biosensors that are dedicated especially to modern medical diagnostics.

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Label-Free Electrochemical Aptasensor for Sensitive Detection of Malachite Green Based on Au Nanoparticle/Graphene Quantum Dots/Tungsten Disulfide Nanocomposites

Cited by 36 publications

References 41 publications

Contribution of Nanomaterials to the Development of Electrochemical Aptasensors for the Detection of Antimicrobial Residues in Food Products

Contribution of Nanomaterials to the Development of Electrochemical Aptasensors for the Detection of Antimicrobial Residues in Food Products

Fluorescence detection of malachite green in fish based on aptamer and SYBR Green I

Modern Electrochemical Biosensing Based on Nucleic Acids and Carbon Nanomaterials

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