Label-free DNA sensor for detection of bladder cancer biomarkers in urine

Shin, Yong Moon; Perera, Agampodi Promoda; Park, Mi Kyoung

doi:10.1016/j.snb.2012.12.057

Cited by 50 publications

(39 citation statements)

References 33 publications

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“…Using microcavity sensors, single nucleotide mismatch discrimination is often achievable under appropriate experimental conditions (99–101). Recently, Shin et al (102) used silicon microring resonators to detect single nucleotide polymorphisms (SNPs) of two commonly mutated genes in bladder cancer, FGFR3 and HRAS, in spiked urine samples. In cases where label free detection of nucleic acids does not provide sufficient signal output, amplification strategies, such as polymerase chain reaction (PCR) can be used.…”

Section: Applications Of Optical Resonatorsmentioning

confidence: 99%

Applications of Optical Microcavity Resonators in Analytical Chemistry

Wade

Bailey

2016

Annual Rev. Anal. Chem.

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Optical resonator sensors are an emerging class of analytical technologies that use recirculating light confined within a microcavity to sensitively measure the surrounding environment. Bolstered by advances in microfabrication, these devices can be configured for a wide variety of chemical or biomolecular sensing applications. The review begins with a brief description of optical resonator sensor operation followed by discussions regarding sensor design, including different geometries, choices of material systems, methods of sensor interrogation, and new approaches to sensor operation. Throughout, key recent developments are highlighted, including advancements in biosensing and other applications of optical sensors. Alternative sensing mechanisms and hybrid sensing devices are then discussed in terms of their potential for more sensitive and rapid analyses. Brief concluding statements offer our perspective on the future of optical microcavity sensors and their promise as versatile detection elements within analytical chemistry.

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Section: Applications Of Optical Resonatorsmentioning

confidence: 99%

Applications of Optical Microcavity Resonators in Analytical Chemistry

Wade

Bailey

2016

Annual Rev. Anal. Chem.

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“…Here, the hybridization between the DNA and the oligonucleotide is exploited for the detection of the DNA-or more specifically-the source of the DNA. Thus, oligonucleotides are most valuable for a variety of targets ranging from pathogens in infectious diseases [42] and food-borne contaminations [43], cancer biomarkers [44], to the diagnosis of genetic diseases by microarraybased multiplexed detection of genes and gene alterations [45][46][47].…”

Section: Dna and Pnamentioning

confidence: 99%

Porous Silicon Biosensors Employing Emerging Capture Probes

Urmann

Tenenbaum

Walter

et al. 2015

Electrochemically Engineered Nanoporous Materials

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The application of porous silicon (PSi) for biosensing was first described by Thust et al. in 1996, demonstrating a potentiometric biosensor for the detection of penicillin. However, only in the past decade PSi has established as a promising nanomaterial for label-free biosensing applications. This chapter focuses on the integration of new emerging capture probes with PSi-based biosensing schemes. An overview of natural and synthetic receptors and their advantageous characteristics for the potential application in PSi biosensors technology is presented. We also review and discuss several examples, which successfully combine these new bioreceptors with PSi optical and electrochemical transducers, for label-free biosensing.

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“…The technique is based on an isothermal solid-phase amplification/detection (ISAD) assay that combines solid-phase-based isothermal DNA amplification (recombinase polymerase amplification (RPA)) and silicon microring resonator (SMR)-based biophotonic sensors, which do not involve labeling (Shin et al, 2013a). Although the SMRs have been used for direct detection of nucleic acids based on the hybridization mechanism between the capture probe and the target (Bogaerts et al, 2012;Baaske and Vollmer, 2012;Shin et al, 2013bShin et al, , 2013cIqbal et al, 2010), however, the MTB-ISAD technique is based on the amplification and the detection mechanism of nucleic acids that was increased the sensitivity of the nucleic acid detection by the combination. Using this strategy, MTB nucleic acid from human specimens is simultaneously amplified and detected by a grafted complementary primer on the SMR in a label-free and real-time manner.…”

Section: Introductionmentioning

confidence: 99%