Label-free detection of protein binding with multisine SPR microchips

Ghosh, Tridib; Williams, L.D.; Mastrangelo, Carlos H.

doi:10.1039/c1lc20260j

Cited by 6 publications

(7 citation statements)

References 13 publications

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“…Usually one analyzes the response to a concentration step in the time domain. However, technical developments based on concentration oscillations and frequency‐domain data processing have also been reported …”

Section: Why and How To Use The Kinetics Of Reactions For Selective mentioning

confidence: 99%

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Kinetics of Reactive Modules Adds Discriminative Dimensions for Selective Cell Imaging

et al. 2016

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Living cells are chemical mixtures of exceptional interest and significance, whose investigation requires the development of powerful analytical tools fulfilling the demanding constraints resulting from their singular features. In particular, multiplexed observation of a large number of molecular targets with high spatiotemporal resolution appears highly desirable. One attractive road to address this analytical challenge relies on engaging the targets in reactions and exploiting the rich kinetic signature of the resulting reactive module, which originates from its topology and its rate constants. This review explores the various facets of this promising strategy. We first emphasize the singularity of the content of a living cell as a chemical mixture and suggest that its multiplexed observation is significant and timely. Then, we show that exploiting the kinetics of analytical processes is relevant to selectively detect a given analyte: upon perturbing the system, the kinetic window associated to response read-out has to be matched with that of the targeted reactive module. Eventually, we introduce the state-of-the-art of cell imaging exploiting protocols based on reaction kinetics and draw some promising perspectives.

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Section: Why and How To Use The Kinetics Of Reactions For Selective mentioning

confidence: 99%

“…Thus, apart from experiments related to easily accessible membrane receptors, we anticipate that in the near future both concentration and temperature will mainly be used as control parameters for in vitro kinetically filtered detection, with surface sensors for instance …”

Section: Concluding Remarks and Perspectivesmentioning

confidence: 99%

Kinetics of Reactive Modules Adds Discriminative Dimensions for Selective Cell Imaging

et al. 2016

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“…When studied compounds interact with aggrecan, they form a complex that modifies the refractive index. This change is measured in real-time and the signal obtained is plotted in resonance unit (RU) versus time (19,20). SPR assays were carried out on a Biacore T200 instrument with CM4 sensor chips (GE Healthcare).…”

Section: Spr Binding Assaysmentioning

confidence: 99%

Proteoglycans as Target for an Innovative Therapeutic Approach in Chondrosarcoma: Preclinical Proof of Concept

Peyrode

Weber

Voissière

et al. 2016

Molecular Cancer Therapeutics

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To date, surgery remains the only option for the treatment of chondrosarcoma, which is radio- and chemoresistant due in part to its large extracellular matrix (ECM) and poor vascularity. In case of unresectable locally advanced or metastatic diseases with a poor prognosis, improving the management of chondrosarcoma still remains a challenge. Our team developed an attractive approach of improvement of the therapeutic index of chemotherapy by targeting proteoglycan (PG)-rich tissues using a quaternary ammonium (QA) function conjugated to melphalan (Mel). First of all, we demonstrated the crucial role of the QA carrier for binding to aggrecan by surface plasmon resonance. In the orthotopic model of Swarm rat chondrosarcoma, an in vivo biodistribution study of Mel and its QA derivative (Mel-QA), radiolabeled with tritium, showed rapid radioactivity accumulation in healthy cartilaginous tissues and tumor after [H]-Mel-QA injection. The higher T/M ratio of the QA derivative suggests some advantage of QA-active targeting of chondrosarcoma. The antitumoral effects were characterized by tumor volume assessment, in vivo Tc-NTP 15-5 scintigraphic imaging of PGs,H-HRMAS NMR spectroscopy, and histology. The conjugation of a QA function to Mel did not hamper its in vivo efficiency and strongly improved the tolerability of Mel leading to a significant decrease of side effects (hematologic analyses and body weight monitoring). Thus, QA conjugation leads to a significant improvement of the therapeutic index, which is essential in oncology and enable repeated cycles of chemotherapy in patients with chondrosarcoma. Mol Cancer Ther; 15(11); 2575-85. ©2016 AACR.

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“…16,17 The two-level PDMS devise is sealed with an SF10 glass slide bearing patterned SPR gold spots using fabrication procedures similar to our previous reports. [5][6][7] Briefly, molds of the two microfluidic layers are made photolithographically on silicon wafers (4 in. diameter) using photoresists AZ9260 and SU-8, respectively.…”

Section: Principles and Schematicsmentioning

confidence: 99%

“…Over the years, SPR based biomolecular interaction analysis (BIA) has gained considerable momentum in pharmaceutical industry where it is used as the secondary drug screening tool. In the instance of step-response method (SRM) [1][2][3][4] and other unconventional schemes [5][6][7] of SPR detection and characterization, bio-sample volumes ranging from micro-liters (ll) to few milliliters (ml) are consumed in one experimental run, increasing the detection time and cost. If the reagent switching technique can be manipulated to transfer optimal volume to the sensing zone, the volume requirements of the experiment can be reduced manifold.…”

mentioning

confidence: 99%

A droplet-based novel approach for viable and low volume consumption surface plasmon resonance bio-sensing inside a polydimethylsiloxane microchip

2013

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Over the course of last two decades, surface plasmon resonance (SPR) has emerged as a viable candidate for label-free detection and characterization for a large pool of biological interactions, ranging from hybridization of oligonucleotides to high throughput drug-screening. Conventional SPR bio-sensing involves a step-response method where the SPR sensorgram in response to a switched sequential flow of analyte and buffer is plotted in real-time and fitted to an exponential curve to extract the associative and dissociative reaction rates. Such measurement schemes involve continuous flow conditions where a substantial reagent volume is consumed and is subject to dispersive mixing at flow switching zones. In this paper, we demonstrate a new plug-train SPR technique in a microfluidic chip that separates and singulates solvent plugs in analyte and buffer by an immiscible air phase. Biosamples are first discretized within plug droplets with volumes in order of few hundred nanoliters or less followed by pressure-driven transport onto SPR sensing sites of this hydrophobically modified SPR microdevise. The kinetic constants k a and k d for a model protein-small molecule interaction pair are extracted from a plugtrain signal and are shown to be in reasonable agreement with our previous reports.

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Label-free detection of protein binding with multisine SPR microchips

Cited by 6 publications

References 13 publications

Kinetics of Reactive Modules Adds Discriminative Dimensions for Selective Cell Imaging

Kinetics of Reactive Modules Adds Discriminative Dimensions for Selective Cell Imaging

Proteoglycans as Target for an Innovative Therapeutic Approach in Chondrosarcoma: Preclinical Proof of Concept

A droplet-based novel approach for viable and low volume consumption surface plasmon resonance bio-sensing inside a polydimethylsiloxane microchip

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