Label-free detection of nosocomial bacteria using a nanophotonic interferometric biosensor

Maldonado, Jesús; Estévez, M.‐Carmen; Gavela, Adrián Fernández; González-López, Juan José; González-Guerrero, Ana Belén; Lechuga, Laura M.

doi:10.1039/c9an01485c

Cited by 55 publications

(42 citation statements)

References 39 publications

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“…For a proper oligonucleotide immobilization, the silicon nitride (Si 3 N 4 ) surface of the BiMW sensor was chemically modified with silane-PEG-COOH in order to generate a homogenous monolayer with anti-fouling properties and exposing reactive carboxyl groups [ 37 ]. For the activation of the silane-PEG-COOH monolayer, we employed the well-known EDC/NHS surface chemistry.…”

Section: Resultsmentioning

confidence: 99%

Ultrasensitive Label-Free Detection of Unamplified Multidrug-Resistance Bacteria Genes with a Bimodal Waveguide Interferometric Biosensor

et al. 2020

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Infections by multidrug-resistant bacteria are becoming a major healthcare emergence with millions of reported cases every year and an increasing incidence of deaths. An advanced diagnostic platform able to directly detect and identify antimicrobial resistance in a faster way than conventional techniques could help in the adoption of early and accurate therapeutic interventions, limiting the actual negative impact on patient outcomes. With this objective, we have developed a new biosensor methodology using an ultrasensitive nanophotonic bimodal waveguide interferometer (BiMW), which allows a rapid and direct detection, without amplification, of two prevalent and clinically relevant Gram-negative antimicrobial resistance encoding sequences: the extended-spectrum betalactamase-encoding gene blaCTX-M-15 and the carbapenemase-encoding gene blaNDM-5 We demonstrate the extreme sensitivity and specificity of our biosensor methodology for the detection of both gene sequences. Our results show that the BiMW biosensor can be employed as an ultrasensitive (attomolar level) and specific diagnostic tool for rapidly (less than 30 min) identifying drug resistance. The BiMW nanobiosensor holds great promise as a powerful tool for the control and management of healthcare-associated infections by multidrug-resistant bacteria.

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Section: Resultsmentioning

confidence: 99%

Ultrasensitive Label-Free Detection of Unamplified Multidrug-Resistance Bacteria Genes with a Bimodal Waveguide Interferometric Biosensor

et al. 2020

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“…In terms of sensitivity, we have also highlighted a number of techniques that successfully use interferometry to amplify the observation of bacterial presence. As is well known, phase-based measurements are among the most sensitive in photonics, so it is no surprise that this strategy is also successful in the detection and monitoring of a small number of bacteria, both for measuring low concentrations [68][69][70] and for assessing bacterial susceptibility to antibiotics [121].…”

Section: Discussionmentioning

confidence: 99%

“…Several improvements have since been made. For example, Maldonado et al [68,69] have demonstrated a bimodal waveguide for the detection of low concentrations of bacteria, that is, down to 40 CFU/mL. The device consists of an optical waveguide in Si 3 N 4 supporting two modes with different polarization, as schematically shown in Figure 2(a).…”

Section: Optical Waveguides and Interferometric Approachesmentioning

confidence: 99%

Nanophotonics for bacterial detection and antimicrobial susceptibility testing

2020

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Photonic biosensors are a major topic of research that continues to make exciting advances. Technology has now improved sufficiently for photonics to enter the realm of microbiology and to allow for the detection of individual bacteria. Here, we discuss the different nanophotonic modalities used in this context and highlight the opportunities they offer for studying bacteria. We critically review examples from the recent literature, starting with an overview of photonic devices for the detection of bacteria, followed by a specific analysis of photonic antimicrobial susceptibility tests. We show that the intrinsic advantage of matching the optical probed volume to that of a single, or a few, bacterial cell, affords improved sensitivity while providing additional insight into single-cell properties. We illustrate our argument by comparing traditional culture-based methods, which we term macroscopic, to microscopic free-space optics and nanoscopic guided-wave optics techniques. Particular attention is devoted to this last class by discussing structures such as photonic crystal cavities, plasmonic nanostructures and interferometric configurations. These structures and associated measurement modalities are assessed in terms of limit of detection, response time and ease of implementation. Existing challenges and issues yet to be addressed will be examined and critically discussed.

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“…Very recent progress on BiMW biosensors reported identifying and quantifying nosocomial bacteria using specific receptors, which involves a silane‐PEG‐COOH coating as the bacteria‐repelling strategy to avoid extra blocking and reach fast (≈12 min) and accurate detection. [ 121 ]…”

Section: Silicon‐based Label‐free Optical Biosensorsmentioning

confidence: 99%

Silicon‐Based Integrated Label‐Free Optofluidic Biosensors: Latest Advances and Roadmap

Wang

Sánchez

Yin

et al. 2020

Adv Materials Technologies

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By virtue of the well‐developed micro‐ and nanofabrication technologies and rapidly progressing surface functionalization strategies, silicon‐based devices have been widely recognized as a highly promising platform for the next‐generation lab‐on‐a‐chip bioanalytical systems with a great potential for point‐of‐care medical diagnostics. Herein, an overview of the latest advances in silicon‐based integrated optofluidic label‐free biosensing technologies relying on the efficient interactions between the evanescent light field at the functionalized surface and specifically bound analytes is presented. State‐of‐the‐art technologies demonstrating label‐free evanescent wave‐based biomarker detection mainly encompass three device configurations, including on‐chip waveguide‐based interferometers, microring resonators, and photonic‐crystal‐based cavities. Moreover, up‐to‐date strategies for elevating the sensitivities and also simplifying the sensing processes are discussed. Emerging laboratory prototypes with advanced integration and packaging schemes incorporating automatic microfluidic components or on‐chip optoelectronic devices lead to one significant step forward in real applications of decentralized diagnostics. Besides, particular attention is paid to currently commercialized label‐free optical bioanalytical models on the market. Finally, the prospects are elaborated with several research routes toward chip‐scale, low‐cost, highly sensitive, multi‐functional, and user‐friendly bioanalytical systems benefiting to global healthcare.

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Label-free detection of nosocomial bacteria using a nanophotonic interferometric biosensor

Abstract: Two methodologies using a BiMW biosensor have been developed for the fast, sensitive detection of P. aeruginosa and MRSA bacteria.

Cited by 55 publications

References 39 publications

Ultrasensitive Label-Free Detection of Unamplified Multidrug-Resistance Bacteria Genes with a Bimodal Waveguide Interferometric Biosensor

Ultrasensitive Label-Free Detection of Unamplified Multidrug-Resistance Bacteria Genes with a Bimodal Waveguide Interferometric Biosensor

Nanophotonics for bacterial detection and antimicrobial susceptibility testing

Silicon‐Based Integrated Label‐Free Optofluidic Biosensors: Latest Advances and Roadmap

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