“…A label-free SHG imaging have been widely used to monitor the fibrillar collagen in specimens of various fibrotic diseases in skin(Chen, Chen et al, 2009), liver(Goh, Leow et al, 2019), lung(Kottmann, Sharp et al, 2015), carotid artery(Megens, oude Egbrink et al, 2008), eye(Tan, Sun et al, 2007), tendon and ligaments(Doras, Taupier et al, 2011). In brain tissue, SHG imaging was used to investigate the structure and polarity of microtubules in neuronal processes(Dombeck, Kasischke et al, 2003, Van Steenbergen, Boesmans et al, 2019), and collagen deposition in glioblastoma(Jiang, Wang et al, 2017). However, to the best of our knowledge, neither the fibrous collagen accumulation and scar formation associated with the brain microinfarcts nor the SHG imaging of them has been reported.…”