Label‐Free Detection of Few Copies of DNA with Carbon Nanotube Impedance Biosensors

Kurkina, Tetiana; Vlandas, Alexis; Ahmad, Ashraf; Kern, Klaus; Balasubramanian, Kannan

doi:10.1002/anie.201006806

Cited by 88 publications

(82 citation statements)

References 29 publications

(32 reference statements)

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“…4(b)) were used to tail after the progress. Compared to CV technique, EIS is much more sensitive for the surface minor changes and used for the very sensitive examination of such biorecognition events at the surface [54][55][56][66][67][68]. Even after three cycles of cell capture/release, the R et remained the 112.9% of the first value (Fig.…”

Section: Nano Resmentioning

confidence: 90%

Electrochemically and DNA-triggered cell release from ferrocene/β-cyclodextrin and aptamer modified dualfunctionalized graphene substrate

Feng

Ren

et al. 2014

Nano Res.

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Here we report a dual-functionalized electrochemical substrate to trigger cancer cells release based on the supramolecular interaction between β-cyclodextrin (β-CD) and Fc on clinical trial II aptamer AS1411 functionalized graphene platform. On one hand, the host-guest interaction can be reversible electrochemically controlled to realize cancer cells capture/release, and 1-adamantylamine binding can further amplify this surface change by competing interaction with β-CD. On the other hand, the AS1411 aptamer and its complementary DNA (cDNA) also can be used as a switchable anchor for cell adhesion. Our work gives an example for label-free, multi-functionalized triggered cell release based on aptamer and β-CD/graphene-modified surface and this multi-ways for cell catch-and-release on graphene modified surface also provides their potential biomedical application.

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Section: Nano Resmentioning

confidence: 90%

Electrochemically and DNA-triggered cell release from ferrocene/β-cyclodextrin and aptamer modified dualfunctionalized graphene substrate

Feng

Ren

et al. 2014

Nano Res.

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“…In these systems, it is only necessary to drop the test solution on the sensor surface and the analyte binding is sensitively recorded by the probe DNA-functionalized nanostructures. Deploying carbon nanotubes, a detection limit in the attomolar range (corresponding to 1,800 molecules in 30 ll) has been reported in buffer solutions [101]. It was possible to achieve this sensitivity even in a heterogeneous solution mixture, where the target DNA comprised only 2% of the total DNA concentration.…”

Section: Nucleic Acid Assaysmentioning

confidence: 88%

“…The chemistry of carbon is quite rich and this has been utilized in a range of methods to functionalize carbon nanotubes [100]. The most successful of these strategies appears to be electrochemical functionalization, which provides the attachment of biomolecules onto carbon surfaces in a very versatile manner in a covalent or noncovalent fashion [78,86,87,101]. Other possibilities include thermally activated chemistry or photochemistry [100].…”

Section: Biofunctionalization Of Nanostructuresmentioning

confidence: 99%

“…To overcome some of these disadvantages, a reference electrode is used as a gate and the sensor signal is measured in an ion-selective field-effect transistor configuration (ISFET). Carbon nanotube-based devices have been realized in such an ISFET configuration without the use of any expensive serial processing technique [84,87,101]. The sensors can be prepared on a large scale and the receptors are attached exclusively on the surface of the nanotubes.…”

Section: Direct Assaysmentioning

confidence: 99%

See 1 more Smart Citation

Towards in vitro molecular diagnostics using nanostructures

Kurkina

Balasubramanian

2011

Cell. Mol. Life Sci.

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Nanostructures appear to be promising for a number of applications in molecular diagnostics, mainly due to the increased surface-to-volume ratio they can offer, the very low limit of detection achievable, and the possibility to fabricate point-of-care diagnostic devices. In this paper, we review examples of the use of nanostructures as diagnostic tools that bring in marked improvements over prevalent classical assays. The focus is laid on the various sensing paradigms that possess the potential or have demonstrated the capability to replace or augment current analytical strategies. We start with a brief introduction of the various types of nanostructures and their physical properties that determine the transduction principle. This is followed by a concise collection of various functionalization protocols used to immobilize biomolecules on the nanostructure surface. The sensing paradigms are discussed in two contexts: the nanostructure acting as a label for detection, or the nanostructure acting as a support upon which the molecular recognition events take place. In order to be successful in the field of molecular diagnostics, it is important that the nanoanalytical tools be evaluated in the appropriate biological environment. The final section of the review compiles such examples, where the nanostructure-based diagnostic tools have been tested on realistic samples such as serum, demonstrating their analytical power even in the presence of complex matrix effects. The ability of nanodiagnostic tools to detect ultralow concentrations of one or more analytes coupled with portability and the use of low sample volumes is expected to have a broad impact in the field of molecular diagnostics.

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“…In addition to CV, electrochemical impedance spectroscopy (EIS) is a highly sensitive method, which we used to track the immobilization with the change of electron transfer resistance (R et ). [30] ITO modified with PDA-RGO and AuNRs showed a low R et value (Figure 1 f). After conjugation with DNA1 and subsequent hybridization with the DNA2-RGD, the R et value increased because the negatively charged phosphate backbone repulsed the redox probe [Fe(CN) 6 ] À3/À4 close to the electrode.…”

mentioning

confidence: 89%

Near‐Infrared‐ and pH‐Responsive System for Reversible Cell Adhesion using Graphene/Gold Nanorods Functionalized with i‐Motif DNA

Wang

Ren

et al. 2013

Angewandte Chemie

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Zelldock: Ein Nahinfrarot(NIR)‐ und pH‐responsives System zum kontrollierten Einfangen und Freisetzen von Zellen nutzt Graphen/Au‐Nanostäbe als Substrat und Doppelstrang‐DNA als schaltbares Verbindungsstück für die Zellimmobilisierung (siehe Schema). Das Substrat bewährte sich in Zyklen von NIR‐Licht und pH‐Änderungen, und die von der Oberfläche freigesetzten Zellen waren nicht beschädigt.

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Label‐Free Detection of Few Copies of DNA with Carbon Nanotube Impedance Biosensors

Cited by 88 publications

References 29 publications

Electrochemically and DNA-triggered cell release from ferrocene/β-cyclodextrin and aptamer modified dualfunctionalized graphene substrate

Electrochemically and DNA-triggered cell release from ferrocene/β-cyclodextrin and aptamer modified dualfunctionalized graphene substrate

Towards in vitro molecular diagnostics using nanostructures

Near‐Infrared‐ and pH‐Responsive System for Reversible Cell Adhesion using Graphene/Gold Nanorods Functionalized with i‐Motif DNA

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