Label-free detection of DNA molecules on the dendron based self-assembled monolayer by electrochemical impedance spectroscopy

Park, Jin‐Young; Kwon, Sung Hong; Park, Chong-Yun; Park, Su‐Moon

doi:10.1016/j.aca.2008.02.055

Cited by 32 publications

(21 citation statements)

References 36 publications

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“…As described above, the PAMAM dendrimer can be utilized for the immobilization of probe DNA or the signal enhancement via target-labeling. Probe DNA of an appropriate density obtained by a cone-shaped dendrimer showed very high specificity to the complementary target, leading to different responses of R ct values in the presence of [Fe(CN) 6 ] 3−/4− depending on the location of the mismatched pair in the duplex [40]. Thus, various kinds of dendrimers can be diversely utilized for DNA sensors by virtue of their controlled sizes, sufficient functional groups, and unique shapes.…”

Section: Dna Sensors Based On Self-assembled Monolayersmentioning

confidence: 99%

DNA Hybridization Sensors Based on Electrochemical Impedance Spectroscopy as a Detection Tool

Park

2009

Sensors

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184

107

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Recent advances in label free DNA hybridization sensors employing electrochemical impedance spectroscopy (EIS) as a detection tool are reviewed. These sensors are based on the modulation of the blocking ability of an electrode modified with a probe DNA by an analyte, i.e., target DNA. The probe DNA is immobilized on a self-assembled monolayer, a conducting polymer film, or a layer of nanostructures on the electrode such that desired probe DNA would selectively hybridize with target DNA. The rate of charge transfer from the electrode thus modified to a redox indicator, e.g., [Fe(CN)6]3−/4−, which is measured by EIS in the form of charge transfer resistance (Rct), is modulated by whether or not, as well as how much, the intended target DNA is selectively hybridized. Efforts made to enhance the selectivity as well as the sensitivity of DNA sensors and to reduce the EIS measurement time are briefly described along with brief future perspectives in developing DNA sensors.

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Section: Dna Sensors Based On Self-assembled Monolayersmentioning

confidence: 99%

DNA Hybridization Sensors Based on Electrochemical Impedance Spectroscopy as a Detection Tool

Park

2009

Sensors

Self Cite

184

107

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“…Besides, as the easy detection of slight changes in antigen binding, EIS was used to represent the electrode surface modifications only by impedance curves . Therefore, it has been widely used in the detection of antibody–antigen, DNA–DNA recognition, and so on . To sum up, Nb and EIS were used for testosterone determination, which offers alternative methods for detection of testosterone.…”

Section: Resultsmentioning

confidence: 99%

Generation of Small Single Domain Nanobody Binders for Sensitive Detection of Testosterone by Electrochemical Impedance Spectroscopy

Zhu

et al. 2016

ACS Appl. Mater. Interfaces

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A phage display library of variable domain of the heavy chain only antibody or nanobody (Nb) was constructed after immunizing a bactrian camel with testosterone. With the smaller molecular size (15 kDa), improved solubility, good stability, high affinity, specificity, and lower immunogenicity, Nbs are a promising tool in the next generation of diagnosis and medical applications. Testosterone is a reproductive hormone, playing an important role in normal cardiac function and being the highly predictive marker for many diseases. Herein, a simple and sensitive immunosensor based on electrochemical impedance spectroscopy (EIS) and Nbs was successfully developed for the determination of testosterone. We successfully isolated the antitestosterone Nbs from an immune phage display library. Moreover, one of the Nbs was biotinylated according to in vivo BirA system, which showed the highest production yield and the most stable case. Further, the EIS immunosensor was set up for testosterone detection by applying the biotinylated antitestosterone Nb. As a result, the biosensor exhibited a linear working range from 0.05 to 5 ng mL(-1) with a detection limit of 0.045 ng mL(-1). In addition, the proposed immunosensor was successfully applied in determining testosterone in serum samples. In conclusion, the proposed immunosensor revealed high specificity of testosterone detection and showed as a potential approach for sensitive and accurate diagnosis of testosterone.

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“…It is important to remark that for obtaining satisfactorily reproduction of experimental impedance data, the capacitor was replaced by a constant phase element. The most widely accepted explanation for the presence of this element is the microscopic roughness of carbon nanotube-based films that causes an inhomogeneous distribution in the solution resistance and the double-layer capacitance [32].…”

Section: Page 9 Of 26mentioning

confidence: 99%

Properties and electrochemical characteristics of boron-doped multi-walled carbon nanotubes

Tsierkezos

Ritter

Thaha

et al. 2015

Chemical Physics Letters

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Label-free detection of DNA molecules on the dendron based self-assembled monolayer by electrochemical impedance spectroscopy

Cited by 32 publications

References 36 publications

DNA Hybridization Sensors Based on Electrochemical Impedance Spectroscopy as a Detection Tool

DNA Hybridization Sensors Based on Electrochemical Impedance Spectroscopy as a Detection Tool

Generation of Small Single Domain Nanobody Binders for Sensitive Detection of Testosterone by Electrochemical Impedance Spectroscopy

Properties and electrochemical characteristics of boron-doped multi-walled carbon nanotubes

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