Label-free detection of C-reactive protein using highly dispersible gold nanoparticles synthesized by reducible biomimetic block copolymers

Iwasaki, Yasuhiko; Kimura, Toshihiro; Orisaka, Masaki; Kawasaki, Hideya; Goda, Tatsuro; Yusa, Shin Ichi

doi:10.1039/c4cc01855a

Cited by 33 publications

(34 citation statements)

References 27 publications

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“…In recent years, many biomimetic synthetic [12][13][14] routes have been proposed to make the synthesized GNPs viable for bioapplication. For example, a variety of amino compounds have been explored as reducing agents which includes amino acids [15,16], primary amines [17], peptides [18][19][20][21][22], and polymers [23].…”

Section: Introductionmentioning

confidence: 99%

Role of Surfactant in the Formation of Gold Nanoparticles in Aqueous Medium

Das

Chadha

Maiti

et al. 2014

Journal of Nanoparticles

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The stability of gold nanoparticles is a major issue which decides their impending usage in nanobiotechnological applications. Often biomimetically synthesized nanoparticles are deemed useless owing to their instability in aqueous medium. So, surfactants are used to stabilize the nanoparticles. But does the surfactant only stabilize by being adsorbed to the surface of the nanoparticles and not play significantly in moulding the size and shape of the nanoparticles? Keeping this idea in mind, gold nanoparticles (GNPs) synthesized by l-tryptophan (Trp) mediated reduction of chloroauric acid (HAuCl 4 ) were stabilized by anionic surfactant, sodium dodecyl sulphate (SDS), and its effect on the moulding of size and properties of the GNPs was studied. Interestingly, unlike most of the gold nanoparticles synthesis mechanism showing saturation growth mechanism, inclusion of SDS in the reaction mixture for GNPs synthesis resulted in a bimodal mechanism which was studied by UV-Vis spectroscopy. The mechanism was further substantiated with transmission electron microscopy. Zeta potential of GNPs solutions was measured to corroborate stability observations recorded visually.

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Section: Introductionmentioning

confidence: 99%

Role of Surfactant in the Formation of Gold Nanoparticles in Aqueous Medium

Das

Chadha

Maiti

et al. 2014

Journal of Nanoparticles

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“…determined systemic CRP levels in the presence of 10% human serum using the biomimetic PC surface and surface plasmon resonance (SPR) . A team of Iwasaki and Goda developed gold nanoparticles (∼10 nm in diameter) covered with MPC polymer in the form of a dense brush for direct colorimetric detection of CRP . Nanocolloids undergo a spontaneous aggregation in the presence of CRP, which results in a red shift by the localized SPR effect.…”

Section: Artificial Ligand For Crpmentioning

confidence: 99%

Critical update on 2‐methacryloyloxyethyl phosphorylcholine (MPC) polymer science

Goda

Ishihara

Miyahara

2015

J of Applied Polymer Sci

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119

105

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2-Methacryloyloxyethyl phosphorylcholine (MPC) is a custom methacrylate with a zwitterionic phosphorylcholine moiety on the side chain. In the past 25 years, MPC has been used as a building block for a wide range of polymeric biomaterials because of its excellent resistance to nonspecific protein adsorption, cell adhesion, and blood coagulation. Recently, MPC polymers with specific features have been used in bioengineering and nanomedicine. This review focuses on three topics that highlight the latest findings on MPC polymers, that is, specific recognition of C-reactive protein (CRP), cell-membrane-penetration abilities, and lubrication properties. These developments will extend the applications of this biomimetic material from bioinert polymers to biosensing, CRP inhibitors, prodrug carriers, subcellular bioimaging, cell manipulation, and joint replacement.

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“…Gold nanoparticles (Au-NPs) have received increasing attention in the past decades because of their chemical inertness, biocompatibility, as well as unique optical and catalytic properties [1][2][3][4]. Surface chemical functionalization of Au-NPs with tactical molecules not only effectively prevents their aggregation but also largely expands their potential applications in various fields such as optical devices, bioimaging, biological labeling, sensors, and catalysis, owing to the synergistic effect between functional molecules and Au-NPs [5][6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%