Label-free detection of biomolecular interaction by optical sensors

Haake, HM; Schütz, Armin; Gauglitz, G.

doi:10.1007/s002160051553

“…However, the attachment of the fluorophores to a biomolecule is a time-and material-consuming process, which can frustrate the need to perform rapid and efficient assessments of the quality of the surface chemistry with respect to its use with specific reagents. Accordingly, for this purpose, a label-free detection method could be an advantageous alternative to fluorescence detection methods [10]. Surface plasmon resonance (SPR) has been widely applied as a label-free detection technique to study the interactions of molecules to functionalized gold surfaces [11][12] as well as to polymer surfaces [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Total internal reflection ellipsometry as a label-free assessment method for optimization of the reactive surface of bioassay devices based on a functionalized cycloolefin polymer

Le

¹

,

Gubala

²

,

Gandhiraman

³

et al. 2010

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. (2010) Total internal reflection ellipsometry as a label-free assessment method for optimization of the reactive surface of bioassay devices based on a functionalized cycloolefin polymer. Analytical and Bioanalytical Chemistry, 398 (5). pp. 1927-1936. ISSN 1618-2642 Abstract:We report a label-free optical detection technique, called total internal reflection ellipsometry (TIRE), which can be applied to study the interactions between biomolecules and a functionalized polymer surface. Zeonor (ZR), a cyclo olefin polymer (COP) with low autofluorescence, high optical transmittance and excellent chemical resistance, is a highly suitable material for optical biosensor platforms due to the ease of fabrication. It can also be modified with a range of reactive chemical groups for surface functionalization. We demonstrate the applications of TIRE in monitoring DNA hybridization assays and human chorionic gonadotrophin (hCG) sandwich immunoassays on the ZR surface functionalized with carboxyl groups. The obtained Y and D spectra after the binding of each layer of analyte have been fitted to a four-layer ellipsometric model to quantitatively determine the amount of analytes bound specifically to the functionalized ZR surface. Our proposed TIRE technique with very low analyte consumption and its microfluidic array format could be a useful tool for evaluating several crucial parameters in immunoassays, DNA interactions, adsorption of biomolecules to solid surfaces or assessment of the reactivity of a functionalized polymer surface toward a specific analyte.

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“…The development of advanced analytical and bioseparation methodologies based on micro-or macroarrays and biosensors is one of the strategic objectives of the so-called postgenomics, but it also has an impact on strategic application fields, such as predictive oncology, diagnostics in the biomedical field, and drug research (Gastrock et al, 2001;Haake et al, 2000;McGlennen, 2001;Rich and Myszka, 2001;Van Regenmortel, 2001). Comprehensive lists of recently published articles describing works employing commercial biosensors and emerging applications in areas of drug discovery, clinical support, food and environment monitoring, and cell membrane biology are available (Rich and Myszka, 2001).…”

Section: Introductionmentioning

confidence: 99%

Levitation and movement of human tumor cells using a printed circuit board device based on software‐controlled dielectrophoresis

Altomare¹,

Borgatti²,

Medoro³

et al. 2003

Biotech & Bioengineering

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In this study we describe an original, efficient, and innovative printed circuit board (PCB) device able to generate dielectrophoresis-based, software-controlled cages that can be moved to any place inside a microchamber. Depending on their dielectrophoretic properties, eukaryotic cells can be "entrapped" in cages and moved under software control. The main conclusion gathered from the experimental data reported is that the PCB device based on dielectrophoresis permits levitation and movement of different tumor cells at different dielectrophoresis conditions. The results presented herein are therefore the basis for experiments aimed at forced interactions or separation of eukaryotic cells using "lab-on-a-chip." In fact, because many cages can be controlled at the same time, and two or more cages can be forced to share the same or a different location, it is possible, in principle, either to bring in contact cells of a differing histotype or to separate them.

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“…Accumulations of material caused by antigen -antibody interactions [187] can also be observed by methods sensitive to changes in layer thickness. Labelfree detection of biomolecular interaction by optical sensors has been reviewed [228]. Further information on optical sensors and their applications can be found in [229][230][231].…”

Section: Optical Sensorsmentioning

confidence: 99%

Ultraviolet and Visible Spectroscopy

Gauglitz

¹

2008

Ullmann's Encyclopedia of Industrial Chemistry

0

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The article contains sections titled: 1. Introduction 1.1. Comparison with Other Spectroscopic Methods 1.2. Development and Uses 2. Theoretical Principles 2.1. Electronic States and Orbitals 2.2. Interaction Between Radiation and Matter 2.2.1. Dispersion 2.2.2. Absorption 2.2.3. Scattering 2.2.4. Reflection 2.2.5. Band Intensity 2.3. The Lambert–BeerLaw 2.3.1. Definitions 2.3.2. Deviations from the Lambert ‐ Beer Law 2.4. Photophysics 2.4.1. Energy Level Diagram 2.4.2. Deactivation Processes 2.4.3. Transition Probability and Fine Structure of the Bands 2.5. Chromophores 2.6. Optical Rotatory Dispersion and Circular Dichroism 2.6.1. Generation of Polarized Radiation 2.6.2. Interaction with Polarized Radiation 2.6.3. Optical Rotatory Dispersion 2.6.4. Circular Dichroism and the Cotton Effect 2.6.5. Magnetooptical Effects 3. Optical Components and Spectrometers 3.1. Principles of Spectrometer Construction 3.1.1. Sequential Measurement of Absorption 3.1.2. Multiplex Methods in Absorption Spectroscopy 3.2. Light Sources 3.2.1. Line Sources 3.2.2. Sources of Continuous Radiation 3.2.3. Lasers 3.3. Selection of Wavelengths 3.3.1. Prism Monochromators 3.3.2. Grating Monochromators 3.3.3. Electro‐Acoustic and Opto‐Acoustic Wavelength Generation 3.4. Polarizers and Analyzers 3.5. Sample Compartments and Cells 3.5.1. Closed Compartments 3.5.2. Modular Arrangements 3.5.3. Open Compartments 3.6. Detectors 3.7. Optical Paths for Special Measuring Requirements 3.7.1. Fluorescence Measurement 3.7.2. Measuring Equipment for Polarimetry, ORD, and CD 3.7.3. Reflection Measurement 3.7.4. Ellipsometry 3.8. Effect of Equipment Parameters 3.9. Connection to Electronic Systems and Computers 4. Uses of UV ‐ VIS Spectroscopy in Absorption, Fluorescence, and Reflection 4.1. Identification of Substances and Determination of Structures 4.2. Quantitative Analysis 4.2.1. Determination of Concentration by Calibration Curves 4.2.2. Classical Multicomponent Analysis 4.2.3. Multivariate Data Analysis 4.2.4. Use in Chromatography 4.3. Fluorimetry 4.3.1. Inner Filter Effects 4.3.2. Fluorescene and Scattering 4.3.3. Excitation Spectra 4.3.4. Applications 4.4. Reflectometry 4.4.1. Diffuse Reflection 4.4.2. Color Measurement 4.4.3. Regular Reflection 4.4.4. Determination of Film Thickness 4.4.5. Ellipsometry 4.5. Resonance Methods 4.5.1. SurfacePlasmon Resonance 4.5.2. Grating Couplers 4.5.3. Other Evanescent Methods 4.5.4. Interferometric Methods 4.6. On‐Line Process Control 4.6.1. Process Analysis 4.6.2. Measurement of Film Thicknesses 4.6.3. Optical Sensors 4.7. Measuring Methods Based on Deviations from the Lambert – Beer Law 5. Special Methods 5.1. Derivative Spectroscopy 5.2. Dual‐Wavelength Spectroscopy 5.3. Scattering 5.3.1. Turbidimetry 5.3.2. Nephelometry 5.3.3. Photon Correlation Spectroscopy 5.4. Luminescence, Excitation, and Depolarization Spectroscopy, and Measurement of Lifetimes 5.5. Polarimetry 5.5.1. Sugar Analysis 5.5.2. Cellulose Determination 5.5.3. Stereochemical StructuralAnalysis 5.5.4. Use of Optical Activity Induced by a Magnetic Field 5.6. Photoacoustic Spectroscopy (PAS)

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Label-free detection of biomolecular interaction by optical sensors

Cited by 74 publications

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Total internal reflection ellipsometry as a label-free assessment method for optimization of the reactive surface of bioassay devices based on a functionalized cycloolefin polymer

Total internal reflection ellipsometry as a label-free assessment method for optimization of the reactive surface of bioassay devices based on a functionalized cycloolefin polymer

Levitation and movement of human tumor cells using a printed circuit board device based on software‐controlled dielectrophoresis

Ultraviolet and Visible Spectroscopy

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