Label-free detection and size estimation of combustion-derived carbonaceous particles in a microfluidic approach

Aslam, Imran; Fron, Eduard; Roeffaers, Maarten B. J.

doi:10.1039/d2na00262k

Cited by 3 publications

(1 citation statement)

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“…Čabanová et al detected amorphous carbon particles from real-life exposure in the mucus and hypertrophic tissue from the human upper respiratory tract using Raman microspectroscopy. , However, Raman microspectroscopy is largely limited by the slow signal acquisition, the sensitivity to fluorescence background (in biomedical samples), and is furthermore challenging for very small particles (<200 nm) . Bové et al have developed a label-free approach to selectively detect BC and CB particles in biological samples based on unique white light (WL) emission using femtosecond (fs) pulsed near-infrared (nIR) laser in a multiphoton microscope. , This technique was used for the detection of CPs in urine and placenta tissue samples, as well as on ivy leaves. − The detection of BC in biological samples based on WL emission is a novel approach; however, it cannot detect BrC nanoparticles (NPs) in biological samples let alone differentiate between BrC and BC.…”

Section: Introductionmentioning

confidence: 99%

Label-Free Identification of Carbonaceous Particles Using Nonlinear Optical Microscopy

et al. 2023

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The adverse health effects of ambient carbonaceous particles (CPs) such as carbon black (CB), black carbon (BC), and brown carbon (BrC) are becoming more evident and depend on their composition and emission source. Therefore, identifying and quantifying these particles in biological samples are important to better understand their toxicity. Here, we report the development of a nonlinear optical approach for the identification of CPs such as CB and BrC using imaging conditions compatible with biomedical samples. The unique visible light fingerprint of CB and BrC nanoparticles (NPs) upon illumination with a femtosecond (fs) pulsed laser at 1300 nm excitation wavelength is an effective approach for their identification in their biological context. The emission from spectral features of these CPs was investigated with time-domain fluorescence lifetime imaging (FLIM) to further support their identification. This study is performed for different types of CPs embedded in agarose gel as well as in in vitro mammalian cells. The unique nonlinear emissive behavior of CP NPs used for their label-free identification is further complementary with fluorophores typically used for specific staining of biological samples thus providing the relevant bio-context.

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Section: Introductionmentioning

confidence: 99%

Label-Free Identification of Carbonaceous Particles Using Nonlinear Optical Microscopy

et al. 2023

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Analysis of black carbon in environmental and biological media: Recent progresses and challenges

Shu,

Huang,

Min

et al. 2023

TrAC Trends in Analytical Chemistry

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Carbonaceous Nanoparticle Air Pollution: Toxicity and Detection in Biological Samples

Aslam

Roeffaers

2022

Nanomaterials

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Among the different air pollutants, particulate matter (PM) is of great concern due to its abundant presence in the atmosphere, which results in adverse effects on the environment and human health. The different components of PM can be classified based on their physicochemical properties. Carbonaceous particles (CPs) constitute a major fraction of ultrafine PM and have the most harmful effects. Herein, we present a detailed overview of the main components of CPs, e.g., carbon black (CB), black carbon (BC), and brown carbon (BrC), from natural and anthropogenic sources. The emission sources and the adverse effects of CPs on the environment and human health are discussed. Particularly, we provide a detailed overview of the reported toxic effects of CPs in the human body, such as respiratory effects, cardiovascular effects, neurodegenerative effects, carcinogenic effects, etc. In addition, we also discuss the challenges faced by and limitations of the available analytical techniques for the qualitative and quantitative detection of CPs in atmospheric and biological samples. Considering the heterogeneous nature of CPs and biological samples, a detailed overview of different analytical techniques for the detection of CPs in (real-exposure) biological samples is also provided. This review provides useful insights into the classification, toxicity, and detection of CPs in biological samples.

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Label-free detection and size estimation of combustion-derived carbonaceous particles in a microfluidic approach

Abstract: Detection and size estimation of combustion-derived carbonaceous particles (CDCPs) is important to understand their toxicity. Size determination of individual nano- and microparticles (NMPs) based on scattered light is a straightforward...

Cited by 3 publications

References 50 publications

Label-Free Identification of Carbonaceous Particles Using Nonlinear Optical Microscopy

Label-Free Identification of Carbonaceous Particles Using Nonlinear Optical Microscopy

Analysis of black carbon in environmental and biological media: Recent progresses and challenges

Carbonaceous Nanoparticle Air Pollution: Toxicity and Detection in Biological Samples

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