Label-free Dengue E protein detection using a functionalized tapered optical fiber sensor

Kamil, Yasmin Mustapha; Bakar, M. H. Abu; Mustapa, M. A.; Yaacob, Mohd Hanif; Abidin, N.H. Zainol; Syahir, Amir; Lee, Hui Jing; Mahdi, Mohd Adzir

doi:10.1016/j.snb.2017.11.005

Cited by 54 publications

(11 citation statements)

References 36 publications

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“…Next, the spectrum was pushed further to the right when PAMAM was added and also after the immobilization of anti-DENV II E protein antibodies with a shift of 0.94 nm and 1.04 nm, respectively. The observations stated was mainly due to the increment of cladding refractive index which brought upon the red shift similar to the behavior reported in previous studies 8,25 . This phenomenon can be explained by looking at the transmission equation of a tapered fiber which is defined as 26 :where I 1 and I 2 are light intensities of two modes which couple together as a result of the tapered dimension to produce I .…”

Section: Resultssupporting

confidence: 88%

“…The affinity of the sensor towards DENV II E proteins was represented by the dissociation constant value at K d = 1.02 × 10 −10 M; R 2 = 0.99. The value obtained was lower than what was reported in 25 supporting positive enhancement through the integration of PAMAM. One of the obvious factors to this would be the larger surface area and more binding sites on the PAMAM molecules.…”

Section: Resultscontrasting

confidence: 82%

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Detection of dengue using PAMAM dendrimer integrated tapered optical fiber sensor

Kamil

Al-Rekabi

Yaacob

et al. 2019

Sci Rep

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The exponential escalation of dengue cases has indeed become a global health crisis. This work elaborates on the development of a biofunctionalized tapered optical fiber (TOF) based sensor with the integration of polyamidoamine (PAMAM) dendrimer for the detection of dengue E protein. The dimension of the TOF generated an evanescent field that was sensitive to any changes in the external medium while the integration of PAMAM promoted more adhesion of bio-recognition molecules; anti-DENV II E protein antibodies; that were complementary to the targeted protein. This in return created more active sites for the absorption of DENV II E proteins onto the tapered region. The resolution and detection limit of the sensor are 19.53 nm/nM and 1 pM, respectively with Kd = 1.02 × 10−10 M.

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Section: Resultssupporting

confidence: 88%

Section: Resultscontrasting

confidence: 82%

Detection of dengue using PAMAM dendrimer integrated tapered optical fiber sensor

Kamil

Al-Rekabi

Yaacob

et al. 2019

Sci Rep

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“…The performance of our SPR sensor is highly dependent on its surface modification, which can cause significant changes in surface mass due to binding events, thus improving its sensing performance. Table 1 presents the performance of other techniques developed for DENV detection, in terms of sensitivity and detection limit [67][68][69][70][71]. From the table, it can be seen that the sensitivity of these techniques has not been extensively evaluated compared to their detection limit.…”

Section: Resultsmentioning

confidence: 99%

Quantitative and Selective Surface Plasmon Resonance Response Based on a Reduced Graphene Oxide–Polyamidoamine Nanocomposite for Detection of Dengue Virus E-Proteins

et al. 2020

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Dengue viral infection is one of the most common deadliest diseases and has become a recurrent issue for public health in tropical countries. Although the spectrum of clinical diagnosis and treatment have recently been established, the efficient and rapid detection of dengue virus (DENV) during viremia and the early febrile phase is still a great challenge. In this study, a dithiobis (succinimidyl undecanoate, DSU)/amine-functionalized reduced graphene oxide-–polyamidoamine dendrimer (DSU/amine-functionalized rGO–PAMAM) thin film-based surface plasmon resonance (SPR) sensor was developed for the detection of DENV 2 E-proteins. Different concentrations of DENV 2 E-proteins were successfully tested by the developed SPR sensor-based system. The performance of the developed sensor showed increased shift in the SPR angle, narrow full-width–half-maximum of the SPR curve, high detection accuracy, excellent figure of merit and signal-to-noise ratio, good sensitivity values in the range of 0.08–0.5 pM (S = 0.2576°/pM, R2 = 0.92), and a high equilibrium association constant (KA) of 7.6452 TM−1. The developed sensor also showed a sensitive and selective response towards DENV 2 E-proteins compared to DENV 1 E-proteins and ZIKV (Zika virus) E-proteins. Overall, it was concluded that the Au/DSU/amine-functionalized rGO–PAMAM thin film-based SPR sensor has potential to serve as a rapid clinical diagnostic tool for DENV infection.

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“…The sensing performances also show high specificity with mismatch DNA strands at 100 nM and 10 nM concentrations. Both studies indicate an opportunity for sensing improvement using taper‐based optical fibers which are able to detect DNA and protein at lower concentrations than 10 nM without any gold catalyst .…”

Section: Introductionmentioning

confidence: 94%

Sensitive Leptospira DNA detection using tapered optical fiber sensor

Zainuddin

Chee

Ahmad

et al. 2018

Journal of Biophotonics

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This paper presents the development of tapered optical fiber sensor to detect a specific Leptospira bacteria DNA. The bacteria causes Leptospirosis, a deadly disease but with common early flu-like symptoms. Optical single mode fiber (SMF) of 125 μm diameter is tapered to produce 12 μm waist diameter and 15 cm length. The novel DNA-based optical fiber sensor is functionalized by incubating the tapered region with sodium hydroxide (NaOH), (3-Aminopropyl) triethoxysilane and glutaraldehyde. Probe DNA is immobilized onto the tapered region and subsequently hybridized by its complementary DNA (cDNA). The transmission spectra of the DNA-based optical fiber sensor are measured in the 1500 to 1600 nm wavelength range. It is discovered that the shift of the wavelength in the SMF sensor is linearly proportional with the increase in the cDNA concentrations from 0.1 to 1.0 nM. The sensitivity of the sensor toward DNA is measured to be 1.2862 nm/nM and able to detect as low as 0.1 fM. The sensor indicates high specificity when only minimal shift is detected for non-cDNA testing. The developed sensor is able to distinguish between actual DNA of Leptospira serovars (Canicola and Copenhageni) against Clostridium difficile (control sample) at very low (femtomolar) target concentrations.

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Label-free Dengue E protein detection using a functionalized tapered optical fiber sensor

Cited by 54 publications

References 36 publications

Detection of dengue using PAMAM dendrimer integrated tapered optical fiber sensor

Detection of dengue using PAMAM dendrimer integrated tapered optical fiber sensor

Quantitative and Selective Surface Plasmon Resonance Response Based on a Reduced Graphene Oxide–Polyamidoamine Nanocomposite for Detection of Dengue Virus E-Proteins

Sensitive Leptospira DNA detection using tapered optical fiber sensor

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