Label‐Free Colorimetric Detection of Single Nucleotide Polymorphism by Using Single‐Walled Carbon Nanotube Intrinsic Peroxidase‐Like Activity

Song, Yujun; Wang, Xiaohui; Zhao, Chao; Qu, Konggang; Ren, Jinsong; Qu, Xiaogang

doi:10.1002/chem.200902643

Cited by 508 publications

(350 citation statements)

References 37 publications

(24 reference statements)

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“…These were consistent with other reports 31 . The non-acute toxicity induced by carboxylated SWNTs can be attributed to the removal of metal catalysts from SWNTs after mixed-acid treatment, which has also been verified in our previous studies 32 .…”

Section: Resultssupporting

confidence: 85%

“…SWNTs (ϕ = 1.1nm, purity > 90%) were purchased from Aldrich (St Louis, MO, USA) and MWNTs (ϕ = 10-20 nm) were purchased from Nanotech Port Co. Ltd. (Shenzhen, China). The preparation and characterization (size and surface charges) of chemically modified CNTs have been described in detail in previous reports [11][12][13][14][15][16][17][18]32 . In brief, the carboxyl- , 2′-O-meRnA or transfection with TRF2 ∆B∆m for 3 weeks, cells were lysed and separated on 10% sDs-PAGE, and probed with anti-p16 InK4a and anti-p21 WAF1 primary antibody, respectively.…”

Section: Methodsmentioning

confidence: 99%

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Insights into the biomedical effects of carboxylated single-wall carbon nanotubes on telomerase and telomeres

et al. 2012

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Both human telomeric G-rich and C-rich DnA have been considered as specific drug targets for cancer therapy. However, due to i-motif structure instability and lack of specific binding agents, it remains unclear whether stabilization of telomeric i-motif can inhibit telomerase activity. single-walled carbon nanotubes (sWnTs) have been reported as the first ligand that can selectively stabilize human telomeric i-motif DnA. Here we report that sWnTs can inhibit telomerase activity through stabilization of i-motif structure. The persistence of i-motif and the concomitant G-quadruplex eventually leads to telomere uncapping and displaces telomerebinding proteins from telomere. The dysfunctional telomere triggers DnA damage response and elicits upregulation of p16 and p21 proteins. This is the first example that sWnTs can inhibit telomerase activity and interfere with the telomere functions in cancer cells. These results provide new insights into understanding the biomedical effects of sWnTs and the biological importance of i-motif DnA.

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Section: Resultssupporting

confidence: 85%

Section: Methodsmentioning

confidence: 99%

Insights into the biomedical effects of carboxylated single-wall carbon nanotubes on telomerase and telomeres

et al. 2012

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“…Owing to the excellent catalytic properties of these nanomaterials, Scrimin and co-workers 7 called them 'nanozymes' in analogy to the nomenclature of catalytically active polymers (synzymes). Magnetic nanoparticles, 4,9 CeO 2 , 6,10-12 V 2 O 5 , 13,14 gold nanoparticles, 7,8,[15][16][17][18] PtPd ÀFe 3 O 4 , 19,20 carbon nanotubes, 21,22 graphene oxide and graphene nanocomposites 23,24 and other types of nanoparticle have been found to exhibit unique enzyme-like catalytic activities and have already shown promising applications in medicine, biotechnology and environmental chemistry.…”

Section: Introductionmentioning

confidence: 99%

Incorporating ATP into biomimetic catalysts for realizing exceptional enzymatic performance over a broad temperature range

Lin

Ren

2014

NPG Asia Mater

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There has been great interest in the development of artificial inorganic nanomaterials that mimic natural peroxidases. Unfortunately, these nanomaterials usually possess relatively low catalytic activity and are generally considered to work effectively only within a narrow temperature range (for example, they are inactive at high temperatures). Intriguingly, adenosine triphosphate (ATP) is not only the ubiquitous energy currency of life, it is also known to form charge transfer complexes with aromatic molecules and to participate in free radical redox chemistry. Inspired by its unique properties, for the first time, we reveal a novel catalytic role for ATP and show that ATP is an ideal boosting agent for markedly improving the catalytic activity of a peroxidase mimic over a broad temperature range and, more significantly, making it possible to achieve exceptionally efficient high-temperature catalytic reactions. These observations pave the way for identifying highly effective modulators that promote the overall performance of artificial enzymes. NPG Asia Materials (2014) 6, e114; doi:10.1038/am.2014.42; published online 18 July 2014 INTRODUCTION Natural enzymes, which are biological molecules of immense catalytic force and high substrate specificity, have attracted considerable attention for use in pharmaceutical processes, agrochemical production, biosensing and food industry applications. 1-3 However, their applications are largely limited by their intrinsic properties, such as the sensitivity of catalytic activity to environmental conditions and low operational stability (owing to denaturation and digestion), as well as by the high costs of preparation and purification. 4,5 To circumvent the aforementioned limitations, tremendous efforts have been made to develop biomimetic catalysts. Among the countless examples of artificial enzymes, the emergence of and recent advances in nanotechnology and biology provide new opportunities for designing functional nanomaterials with enzyme-like characteristics. 4,[6][7][8] These materials might serve as novel and promising artificial enzymes with the advantages of facile preparation, tunability of catalytic activity and high stability under stringent conditions. Owing to the excellent catalytic properties of these nanomaterials, Scrimin and co-workers 7 called them 'nanozymes' in analogy to the nomenclature of catalytically active polymers (synzymes). Magnetic nanoparticles, 4,9 CeO 2 , 6,10-12 V 2 O 5 , 13,14 gold nanoparticles, 7,8,[15][16][17][18] PtPd ÀFe 3 O 4 , 19,20 carbon nanotubes, 21,22 graphene oxide and graphene nanocomposites 23,24 and other types of nanoparticle have been found to exhibit unique enzyme-like catalytic activities and have

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“…For example, Song and co-workers applied intrinsic peroxidase-like activity of single-walled carbon nanotubes (SWNTs) to detect nucleic acid targets with a simple colorimetry [12]. Similarly to the HRP enzyme described above, SWNTs catalyze the reaction of the peroxidase substrate (TMB) giving the blue oxidized TMB product (oxTMB) when H2O2 is present.…”

Section: Enzyme Labeling Strategies Without Polymerase Target Amplifimentioning

confidence: 99%

“…Similarly to the HRP enzyme described above, SWNTs catalyze the reaction of the peroxidase substrate (TMB) giving the blue oxidized TMB product (oxTMB) when H2O2 is present. Using this approach, single-stranded (ss) DNA targets were detected in a complex with SWNTs in just 15 min at 1 nM concentration of DNA (12).…”

Section: Enzyme Labeling Strategies Without Polymerase Target Amplifimentioning

confidence: 99%

Novel Signal-Enhancing Approaches for Optical Detection of Nucleic Acids—Going beyond Target Amplification

Miotke

Barducci²,

Astakhova³

2015

Chemosensors

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Detection of low-abundance nucleic acids is a challenging task, which over the last two decades has been solved using enzymatic target amplification. Enzymatic synthesis enhances the signal so that diverse, scientifically and clinically relevant molecules can be identified and studied, including cancer DNA, viral nucleic acids, and regulatory RNAs. However, using enzymes increases the detection time and cost, not to mention the high risk of mistakes with amplification and data alignment. These limitations have stimulated a growing interest in enzyme-free methods within researchers and industry. In this review we discuss recent advances in signal-enhancing approaches aimed at nucleic acid diagnostics that do not require target amplification. Regardless of enzyme usage, signal enhancement is crucial for the reliable detection of nucleic acids at low concentrations. We pay special attention to novel nanomaterials, fluorescence microscopy, and technical advances in detectors for optical assessment. We summarize sensitivity parameters of the currently available assays and devices which makes this review relevant to the broad spectrum of researchers working in fields from biophysics, to engineering, to synthetic biology and bioorganic chemistry.

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Label‐Free Colorimetric Detection of Single Nucleotide Polymorphism by Using Single‐Walled Carbon Nanotube Intrinsic Peroxidase‐Like Activity

Cited by 508 publications

References 37 publications

Insights into the biomedical effects of carboxylated single-wall carbon nanotubes on telomerase and telomeres

Insights into the biomedical effects of carboxylated single-wall carbon nanotubes on telomerase and telomeres

Incorporating ATP into biomimetic catalysts for realizing exceptional enzymatic performance over a broad temperature range

Novel Signal-Enhancing Approaches for Optical Detection of Nucleic Acids—Going beyond Target Amplification

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