Label-free colorimetric detection of deoxyribonuclease I activity based on the DNA-enhanced peroxidase-like activity of MIL-53(Fe)

Song, Chan; Ding, Wei; Li, Haibo; Zhao, Weiwen; Yao, Yue; Yao, Cheng

doi:10.1039/c9nj03006a

Cited by 20 publications

(16 citation statements)

References 39 publications

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“…For example, compared with other shapes of Mn 3 O 4 nanoparticles (i.e., flower-like, polyhedron, spinel), octahedral Mn 3 O 4 nanozymes had more regular surfaces to interact with ssDNA, resulting in a significant inhibition of oxidase-like activity by ssDNA [ 40 ]. Adsorption is the first and key step for realizing DNA regulation of nanozyme activity [ 45 , 46 ]. Different nanomaterials have different kinds of adsorption force to ssDNA [ 47 ].…”

Section: Factors Affecting the Regulation By Ssdna Of Nanozyme Activitymentioning

confidence: 99%

“…The usual trend of nanozyme activity was to first increase and then decrease with the increase in ssDNA concentration [ 52 , 53 , 54 ]. However, some data support a different variation trend of nanozyme activity, which is to first increase and then keep stable with the increase of ssDNA concentration [ 46 , 50 , 51 , 55 , 56 ]. The reason for the two different trends was unclear.…”

Section: Factors Affecting the Regulation By Ssdna Of Nanozyme Activitymentioning

confidence: 99%

“…Protonated cytosine nucleotides in buffer at pH 4.0 increased the electrostatic repulsion between cytosine and positively charged TMB, resulting in a lower affinity of MoS 2 nanozymes [ 51 ]. Guanine (G) was also reported to significantly enhance the peroxidase-like activity of WS 2 nanosheets [ 57 ], iron-based MOFs modified with acidized carbon nanotubes (MOF/CNTs) [ 52 ], and MIL-53(Fe) [ 46 ]. Purine (A, G) modification demonstrated a remarkable enhancement of the peroxidase-like activity of AuNPs, while pyrimidine (T, C) modification enhanced it slightly, which was attributed to the difference in the interaction between TMB and the surface-adsorbed nucleobases [ 58 ].…”

Section: Factors Affecting the Regulation By Ssdna Of Nanozyme Activitymentioning

confidence: 99%

“…Besides the intrinsic factors mentioned above, the reaction conditions were also very important for DNA to regulate the nanozyme activity. The buffer pH at 4.0 had been found to be the optimum condition for both the catalytic activity of nanozymes and the inhibition of nanozyme activity by ssDNA [ 45 , 46 , 56 , 57 ]. TMB was the most-used substrate in those literatures.…”

Section: Factors Affecting the Regulation By Ssdna Of Nanozyme Activitymentioning

confidence: 99%

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Regulation Mechanism of ssDNA Aptamer in Nanozymes and Application of Nanozyme-Based Aptasensors in Food Safety

Wang

Zhou

et al. 2022

Foods

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Food safety issues are a worldwide concern. Pathogens, toxins, pesticides, veterinary drugs, heavy metals, and illegal additives are frequently reported to contaminate food and pose a serious threat to human health. Conventional detection methods have difficulties fulfilling the requirements for food development in a modern society. Therefore, novel rapid detection methods are urgently needed for on-site and rapid screening of massive food samples. Due to the extraordinary properties of nanozymes and aptamers, biosensors composed of both of them provide considerable advantages in analytical performances, including sensitivity, specificity, repeatability, and accuracy. They are considered a promising complementary detection method on top of conventional ones for the rapid and accurate detection of food contaminants. In recent years, we have witnessed a flourishing of analytical strategies based on aptamers and nanozymes for the detection of food contaminants, especially novel detection models based on the regulation by single-stranded DNA (ssDNA) of nanozyme activity. However, the applications of nanozyme-based aptasensors in food safety are seldom reviewed. Thus, this paper aims to provide a comprehensive review on nanozyme-based aptasensors in food safety, which are arranged according to the different interaction modes of ssDNA and nanozymes: aptasensors based on nanozyme activity either inhibited or enhanced by ssDNA, nanozymes as signal tags, and other methods. Before introducing the nanozyme-based aptasensors, the regulation by ssDNA of nanozyme activity via diverse factors is discussed systematically for precisely tailoring nanozyme activity in biosensors. Furthermore, current challenges are emphasized, and future perspectives are discussed.

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Section: Factors Affecting the Regulation By Ssdna Of Nanozyme Activitymentioning

confidence: 99%

Section: Factors Affecting the Regulation By Ssdna Of Nanozyme Activitymentioning

confidence: 99%

Section: Factors Affecting the Regulation By Ssdna Of Nanozyme Activitymentioning

confidence: 99%

Section: Factors Affecting the Regulation By Ssdna Of Nanozyme Activitymentioning

confidence: 99%

See 2 more Smart Citations

Regulation Mechanism of ssDNA Aptamer in Nanozymes and Application of Nanozyme-Based Aptasensors in Food Safety

Wang

Zhou

et al. 2022

Foods

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show abstract

“…Further, unlike our present work where the sensing performance is demonstrated in neutral pH, a major proportion of MOF-based optical sensors have displayed peroxidase-like activity only in the mildly acidic range (pH 4−5), which limits the application of such materials in physiological systems. [29,31,83] Very few reports have demonstrated satisfactory sensing performance of MOFs-based optical sensors at neutral pH condition. [10] In the current work, an appreciable intrinsic peroxidase-like activity was exhibited by Fe-BTC/ PEDOT > Fe-BTC > Fe-BTC/PpPDA in the decreasing order of activity (Figure 4) at neutral pH.…”

Section: Optical Studymentioning

confidence: 99%

An Investigation into the Intrinsic Peroxidase‐Like Activity of Fe‐MOFs and Fe‐MOFs/Polymer Composites

Thakur

Karve

Sun

et al. 2021

Adv Materials Technologies

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The quest for developing materials that provide a perfect trade‐off between factors such as enzyme‐like response, stability, and low cost has been a long‐standing challenge in the field of biosensing. Metal‐organic frameworks (MOFs) and their composites have emerged as promising candidates for biosensing applications due to their exceptional properties such as tunable pore size, high specific surface area, and exposed active sites. A comparative study of the intrinsic peroxidase‐like activity of five different MOF materials: Fe‐BTC, NH2‐MIL‐101(Fe), composites of Fe‐BTC with polymers polydopamine (PDA), poly p‐phenylenediamine (PpPDA), and poly(3,4‐ethylenedioxythiophene) is reported for the detection of H2O2, an important biomarker in biomedical diagnostics. The response of these materials toward H2O2 via electrochemical and colorimetric techniques is mapped and it is found that, at neutral pH, the Fe‐BTC/PEDOT composite exhibits the highest sensitivity and activity compared to other MOF materials in this study. The results indicate that the combination of unique properties of Fe‐BTC and conducting polymer PEDOT, improves the peroxidase‐like activity of the Fe‐BTC/PEDOT composite compared to the individual components. This work presents a new opportunity for easy‐to synthesize and low‐cost MOFs/conducting polymer composites for biosensing applications and provides significant insights to achieve rational design of composites with desirable functional properties

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When nanozymes meet deoxyribonucleic acid: Understanding their interactions and biomedical diagnosis applications

Liang,

Chen,

et al. 2024

Interdisciplinary Medicine

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As emerging alternatives to natural enzymes, nanoscale materials featuring enzyme‐like catalytic behaviors (nanozymes) exhibit some attractive merits including robust activity, low cost, and easy‐to‐regulate performance. These merits have enabled them to be intensively used in the biomedical field in recent years. To remedy the lack of catalytic selectivity in most nanozymes, deoxyribonucleic acid (DNA) chains with specific recognition functions are utilized to integrate with nanozymes to produce various nanozyme–DNA combinations via adsorption/desorption. In the formed combinations, the DNA component provides the molecular/ionic recognition role, and the nanozyme part offers response with catalytically amplified signals, enabling them to detect analytes and biomarkers selectively and sensitively. To highlight this interesting topic, here we made a critical review of the interactions between nanozymes and DNA and their applications in biosensing and disease diagnosis. First, strategies for the conjugation of DNA chains onto nanozyme surface were introduced briefly. Then, the interactions between DNA and nanozymes were summarized in detail, where flexible modulations of nanozyme activity by DNA adsorption/desorption as well as various factors were analyzed, and potential impacts caused by nanozymes on the recognition characteristics of DNA chains were pointed out. After that, typical applications of DNA‐mediated nanozyme modulation in toxic ion sensing, health risk factor monitoring, and biomedical diagnosis were introduced. In the end, prospects of the combination of nanozymes and DNA chains were presented, and future challenges of the emerging field were also discussed, to attract more interest and effort to advance this promising area.

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Label-free colorimetric detection of deoxyribonuclease I activity based on the DNA-enhanced peroxidase-like activity of MIL-53(Fe)

Abstract: In this study, the effect of single-stranded DNA (ssDNA) on the intrinsic peroxidase-like activity of MIL-53(Fe) was investigated.

Cited by 20 publications

References 39 publications

Regulation Mechanism of ssDNA Aptamer in Nanozymes and Application of Nanozyme-Based Aptasensors in Food Safety

Regulation Mechanism of ssDNA Aptamer in Nanozymes and Application of Nanozyme-Based Aptasensors in Food Safety

An Investigation into the Intrinsic Peroxidase‐Like Activity of Fe‐MOFs and Fe‐MOFs/Polymer Composites

When nanozymes meet deoxyribonucleic acid: Understanding their interactions and biomedical diagnosis applications

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