2023
DOI: 10.1101/2023.08.04.551932
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Label-free citrate-stabilized silver nanoparticles-based highly sensitive, cost-effective and rapid visual method for differential detection ofMycobacterium tuberculosisandMycobacterium bovis

Naresh Patnaik,
Ruchi Jain Dey

Abstract: Tuberculosis poses a global health challenge, demanding improved diagnostics and therapies. Distinguishing between Mycobacterium tuberculosis (M. tb) and Mycobacterium bovis (M. bovis) infections holds critical "One Health" significance due to zoonotic nature of these infections and inherent resistance of M. bovis to pyrazinamide, a key part of Directly Observed Treatment, Short-course (DOTS) regimen. Furthermore, most of the currently used molecular detection methods fail to distinguish between the two specie… Show more

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Cited by 1 publication
(4 citation statements)
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“…The RPA technique for isothermal DNA amplification, distinguishes itself from PCR by the virtue of its speed of operation (15-20 min) at a constant temperature range (∼25 - 42°C) compared to other isothermal techniques like LAMP and MCDA requiring more time (∼30 – 60 min) and higher temperature (60 - 65 °C). AgNP-based assay reported by us previously for differential molecular detection of Mycobacterium tuberculosis and M. bovis 29 , offers unique advantage of visual detection in a highly sensitive and cost-effective manner. The synergy of RPA and the AgNP assay eliminates the need for expensive thermal cycling equipment, centrifuges, electrophoresis equipment, gel documentation facilities, and real-time qPCR machines, making RPA particularly suitable for field and POC applications.…”
Section: Discussionmentioning
confidence: 99%
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“…The RPA technique for isothermal DNA amplification, distinguishes itself from PCR by the virtue of its speed of operation (15-20 min) at a constant temperature range (∼25 - 42°C) compared to other isothermal techniques like LAMP and MCDA requiring more time (∼30 – 60 min) and higher temperature (60 - 65 °C). AgNP-based assay reported by us previously for differential molecular detection of Mycobacterium tuberculosis and M. bovis 29 , offers unique advantage of visual detection in a highly sensitive and cost-effective manner. The synergy of RPA and the AgNP assay eliminates the need for expensive thermal cycling equipment, centrifuges, electrophoresis equipment, gel documentation facilities, and real-time qPCR machines, making RPA particularly suitable for field and POC applications.…”
Section: Discussionmentioning
confidence: 99%
“…Bridging flocculation assay and AgNP-based visual detection assays were performed using PCR amplified products as described by us previously 29 . Briefly, paramagnetic beads work with a principle that they adsorb the amplified DNA molecules and flocculate upon addition of flocculation buffer (sodium acetate and tween 20 at an acidic pH) due to the formation of DNA bridges connecting the paramagnetic beads.…”
Section: Methodsmentioning
confidence: 99%
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