Label-Free Aptamer Nanogold Resonance Scattering Method for Trace Alkaline Phosphatase Coupling the Adenosine Triphosphate Reaction

Graphical abstract:The ratiometric fluorescent probe herein can detect ALP levels in clinical serum samples, and spatially map endogenous ALP level variation as a result of drug induced organ damages. ARTICLE This journal isClinical drug-induced organ toxicities and damages have been recognized as an important public health issue, and the effective approach capable of in vivo detecting biomarkers resulted from drug-induced organ damage is actively pursued. Herein we demonstrate a ratiometric fluorescent probe which can trace alkaline phosphatase (ALP, an organ damage biomarker) level variation in a spatial manner in vivo. The probe was synthesized by incorporatin g a phosphate group and an amine-N-oxide group on a 1,8-naphthalimide derivative. The presence of ALP cleaves the phosphate group from naphthalimide and remarkably alters the probe's photophysical properties, thus achieving ratiometric detection for ALP. The incorporation of amine-N-oxide ensures excellent water solubility and biocompatibility, which guarantees the ratiometric detection of ALP in aqueous media and in the cells overexpressed with ALP. With a detection limit of 0.38 U/L, the probe was successfully used in detecting ALP in human serum samples. Moreover, the probe can be employed to monitor and spatially map the endogenous ALP level variation in zebrafishes. This is the first observation, to our knowledge, of organ-scale ALP pattern in vivo as a result of clinical drug (APAP) induced damages.

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A ratiometric fluorescent probe for in vivo tracking of alkaline phosphatase level variation resulting from drug-induced organ damage

Hou

Zeng

et al. 2015

J. Mater. Chem. B

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Label-Free Aptamer Nanogold Resonance Scattering Method for Trace Alkaline Phosphatase Coupling the Adenosine Triphosphate Reaction

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A ratiometric fluorescent probe for in vivo tracking of alkaline phosphatase level variation resulting from drug-induced organ damage

A ratiometric fluorescent probe for in vivo tracking of alkaline phosphatase level variation resulting from drug-induced organ damage

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