Label-free and enzyme-free colorimetric detection of microRNA by catalyzed hairpin assembly coupled with hybridization chain reaction

Wu, Hao; Liu, Yaling; Wang, Hongyong; Wu, Jun; Zhu, Feifan; Pei, Zingway

doi:10.1016/j.bios.2016.03.013

Cited by 115 publications

(37 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Device-based analytical strategies mainly include electrochemical sensors and MEMS [14,15]. Methods-based approaches mainly include fluorescence, colorimetric, and luminescence assays [16,17,18,19,20,21,22,23,24,25,26,27,28]. Among these, the fluorometric method is an excellent choice for the quantitative detection of miRNAs due to its high sensitivity and convenience of operation.…”

Section: Introductionmentioning

confidence: 99%

An Exonuclease I-Based Quencher-Free Fluorescent Method Using DNA Hairpin Probes for Rapid Detection of MicroRNA

Liu

et al. 2017

Sensors

View full text Add to dashboard Cite

MicroRNAs (miRNAs) act as biomarkers for the diagnosis of a variety of cancers. Since the currently used methods for miRNA detection have limitations, simple, sensitive, and cost-effective methods for the detection of miRNA are required. This work demonstrates a facile, quencher-free, fluorescence-based analytical method for cost-effective and sensitive detection of miRNA using a super 2-aminopurine (2-AP)-labeled hairpin probe (HP) and exonuclease I activity. Specifically, the fluorescence of 2-AP is strongly quenched when it is incorporated within DNA. In the presence of a target miRNA, HP attains an open conformation by hybridizing with the target miRNA to form a double-stranded structure with a protruding 3′-terminus. Next, the digestion of the protruding 3′-terminus is triggered by exonuclease I, during which 2-AP is released free in solution from the DNA, thereby increasing fluorescence. This method is highly sensitive, with a detection limit of 0.5 nM—10 times lower than a previously reported quencher-free fluorescence method. Furthermore, this method has potential applications in clinical diagnosis and biomedical research.

show abstract

Section: Introductionmentioning

confidence: 99%

An Exonuclease I-Based Quencher-Free Fluorescent Method Using DNA Hairpin Probes for Rapid Detection of MicroRNA

Liu

et al. 2017

Sensors

View full text Add to dashboard Cite

show abstract

“…This analytical platform allows the sensing of target DNA with a detection limit of 0.1 pM. This amplification platform has also been used in detecting Hg 2+ [127] and microRNA [128] with a detection limit of 9.7 pM and 7.4 fM, respectively.…”

Section: Peroxidase-mimicking Dnazyme (G4-dnazyme) As Colorimetricmentioning

confidence: 99%

Integrating Deoxyribozymes into Colorimetric Sensing Platforms

Chang

Zakaria

Deng

et al. 2016

Sensors

View full text Add to dashboard Cite

Biosensors are analytical devices that have found a variety of applications in medical diagnostics, food quality control, environmental monitoring and biodefense. In recent years, functional nucleic acids, such as aptamers and nucleic acid enzymes, have shown great potential in biosensor development due to their excellent ability in target recognition and catalysis. Deoxyribozymes (or DNAzymes) are single-stranded DNA molecules with catalytic activity and can be isolated to recognize a wide range of analytes through the process of in vitro selection. By using various signal transduction mechanisms, DNAzymes can be engineered into fluorescent, colorimetric, electrochemical and chemiluminescent biosensors. Among them, colorimetric sensors represent an attractive option as the signal can be easily detected by the naked eye. This reduces reliance on complex and expensive equipment. In this review, we will discuss the recent progress in the development of colorimetric biosensors that make use of DNAzymes and the prospect of employing these sensors in a range of chemical and biological applications.

show abstract

“…Compared with natural enzymes, artificial enzymes, which have been extensively utilized for bioanalytical applications, have several merits owing to the flexible base sequences as well as nonspecific binding properties. 24,25 Among them, the G-quadruplexhemin complex, formed by hemin intercalated into the guanine-rich nucleic acid sequences, [26][27][28] can catalyze H 2 O 2 -mediated oxidation of reductive substrates and has been successfully employed for the electrochemical, 29,30 colorimetric, and chemiluminescent determination of nucleotides. [31][32][33][34][35] By combined application with hybridization chain reaction (HCR), isothermal exponential amplification reaction (EXPAR), and rolling circle amplification (RCA) strategy, G-quadruplex-hemin complexes have been successfully employed to fabricate biosensors.…”

Section: Introductionmentioning

confidence: 99%

Proximity hybridization-mediated isothermal exponential amplification for ultrasensitive electrochemical protein detection

Yu¹,

Su²,

Zhu³

et al. 2017

IJN

View full text Add to dashboard Cite

In this study, we fabricated a novel electrochemical biosensing platform on the basis of target-triggered proximity hybridization-mediated isothermal exponential amplification reaction (EXPAR) for ultrasensitive protein analysis. Through rational design, the aptamers for protein recognition were integrated within two DNA probes. Via proximity hybridization principle, the affinity protein-binding event was converted into DNA assembly process. The recognition of protein by aptamers can trigger the strand displacement through the increase of the local concentrations of the involved probes. As a consequence, the output DNA was displaced, which can hybridize with the duplex probes immobilized on the electrode surface subsequently, leading to the initiation of the EXPAR as well as the cleavage of duplex probes. Each cleavage will release the gold nanoparticles (AuNPs) binding sequence. With the modification of G-quadruplex sequence, electrochemical signals were yielded by the AuNPs through oxidizing 3,3′,5,5′-tetramethylbenzidine in the presence of H 2 O 2 . The study we proposed exhibited high sensitivity toward platelet-derived growth factor BB (PDGF-BB) with the detection limit of 52 fM. And, this method also showed great selectivity among the PDGF isoforms and performed well in spiked human serum samples.

show abstract

Label-free and enzyme-free colorimetric detection of microRNA by catalyzed hairpin assembly coupled with hybridization chain reaction

Cited by 115 publications

References 34 publications

An Exonuclease I-Based Quencher-Free Fluorescent Method Using DNA Hairpin Probes for Rapid Detection of MicroRNA

An Exonuclease I-Based Quencher-Free Fluorescent Method Using DNA Hairpin Probes for Rapid Detection of MicroRNA

Integrating Deoxyribozymes into Colorimetric Sensing Platforms

Proximity hybridization-mediated isothermal exponential amplification for ultrasensitive electrochemical protein detection

Contact Info

Product

Resources

About