Label-free and culture-free microbe detection by three dimensional hot-junctions of flexible Raman-enhancing nanohybrid platelets

Liu, Tingyu; Ho, Jun-Ying; Wei, Jiun-Chiou; Cheng, Wei-Chih; Chen, I.-Hui; Shiue, Jessie; Wang, Huai-Hsien; Wang, Juen-Kai; Wang, Yuh‐Lin; Lin, Jiang‐Jen

doi:10.1039/c3tb21469a

Cited by 31 publications

(22 citation statements)

References 40 publications

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“…Due to their advantageous chemical properties, it has been posited that such clays can form effective adsorbents for stabilizing heavy-metal NPs [33,34,35]. Recently, 3D hot-junctions of AgNP/2D nanomaterial hybrids have been used as SERS substrates for various biochemical analyses [36,37,38,39].…”

Section: Introductionmentioning

confidence: 99%

Immobilization and 3D Hot-Junction Formation of Gold Nanoparticles on Two-Dimensional Silicate Nanoplatelets as Substrates for High-Efficiency Surface-Enhanced Raman Scattering Detection

Lee

Chiu

2019

Nanomaterials

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We synthesize a high-efficiency substrate for surface-enhanced Raman scattering (SERS) measurements, which is composed of gold nanoparticles (AuNPs) on two-dimensional silicate nanoplatelets acting as an inorganic stabilizer, via the in-situ reduction of hydrogen tetrachloroaurate (III) by sodium citrate in an aqueous solution. Silicate platelets of ~1-nm thickness and various sizes, viz. laponite (50 nm), sodium montmorillonite (Na+–MMT, 100 nm), and mica (500 nm), are used to stabilize the AuNPs (Au@silicate), which are formed with uniform diameters ranging between 25 and 30 nm as confirmed by transmission electron microscopy (TEM). In particular, the laponite SERS substrate can be used in biological, environmental, and food safety applications to measure small molecules such as DNA (adenine molecule), dye (Direct Blue), and herbicide (paraquat) as it shows high detection sensitivity with a detection limit of 10−9 M for adenine detection. These highly sensitive SERS substrates, with their three-dimensional hot-junctions formed with AuNPs and two-dimensional silicate nanoplatelets, allow the highly efficient detection of organic molecules. Therefore, these Au@silicate nanohybrid substrates have great potential in biosensor technology because of their environmentally-friendly and simple fabrication process, high efficiency, and the possibility of rapid detection.

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Section: Introductionmentioning

confidence: 99%

Immobilization and 3D Hot-Junction Formation of Gold Nanoparticles on Two-Dimensional Silicate Nanoplatelets as Substrates for High-Efficiency Surface-Enhanced Raman Scattering Detection

Lee

Chiu

2019

Nanomaterials

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“…With that in mind, laser excitation would bring about surface plasmon resonances in AuNP arrays of honeycomb-like polymeric SERS substrates. As a result, the Raman signals of analytes multiplied and produced enormous SERS enhancement, elucidating the multiplied hot-spots effect [ 40 , 41 ].…”

Section: Resultsmentioning

confidence: 99%

“…However, the detailed causes for such enhanced phenomena were not revealed. Therefore, we set out to investigate the mechanism of Raman enhancement by hot-spots resonance effects in the three-dimensional (3D) honeycomb porous structure along with the flat-film substrates as a control [ 23 , 40 , 41 ].…”

Section: Introductionmentioning

confidence: 99%

Au Nanoparticles Immobilized on Honeycomb-Like Polymeric Films for Surface-Enhanced Raman Scattering (SERS) Detection

Chiang

Liu

et al. 2017

Polymers

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Abstract:We have successfully developed novel surface-enhanced Raman scattering (SERS) substrates with three-dimensional (3D) porous structures for effectively improving the sensitivity and reproducibility of SERS, which can rapidly detect small molecules (rhodamine 6G as an example). Periodical arrays of the honeycomb-like substrates were fabricated by self-assembling polyurethane-co-azetidine-2,4-dione (PU-PAZ) polymers. PU-PAZ comprising amphiphilic dendrons could stabilize the phase separation between the water droplets and polymer solution, and then organize into regular porous structures during the breath figure method. Subsequently, SERS substrates were fabricated by immobilizing gold nanoparticles (AuNPs) onto the honeycomb-like films with various 3D porous structures, controlled by the different PU-PAZ concentrations and relative humidities. Results show that surface enhancement factors of honeycomb-like substrates were 20 times higher than that of flat-film substrates (control group) due to enormous hot-spots resonance effects by the 3D porous structure, verified through Raman mapping at various positions of the z-axis. Furthermore, the particle size effects were evaluated by immobilized 12 and 67 nm of AuNPs on the honeycomb-like substrates, indicating larger AuNPs could induce more pronounced hot-spots effects. The generation of hot-spots resonance to enhance Raman intensity is strongly dependent on the diameter of AuNPs and the pore size of the honeycomb-like and 3D porous substrates for label-free and rapid SERS detection.

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“…2D layered mineral nanosheets exhibit highly tuneable properties and are suitable candidates for applications in a large number of fields such as drug delivery, flame retardant, nanohybrids, antibacterial, rheology modifiers, superior thermal transporter, and food packaging . Recently, there have been an enormous number of new developments using 2D layered minerals intercalated with polymer chains to load and in vivo deliver biologically active materials such as porphyrins, nucleoside phosphates, drugs, vitamins, amino acids, and fatty acids .…”

Section: Applicationsmentioning

confidence: 99%

A Review on Layered Mineral Nanosheets Intercalated with Hydrophobic/Hydrophilic Polymers and Their Applications

Sangian

Naficy

Dehghani

et al. 2018

Macro Chemistry & Physics

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Materials with layered structures at the nanoscale have lately drawn significant attention from engineers and scientists in the fields of physics, chemistry, and mathematics, due to the unique characteristics that originate from their hierarchical structure. The nanosized free space between each two adjacent layers, so‐called the interlayer space, is an attractive place that can be manipulated by incorporating different molecular species to generate novel physical and mechanical behaviors. This review highlights the latest studies and new important developments on possible methods of intercalating popular species such as hydrophobic and hydrophilic polymers into the interlayer spaces of layered materials. It also provides a description of intercalation processes as well as final applications for better understanding, as it is believed to be an effective factor in utilizing these materials in research and industry. Finally, this review gives perspectives on the future applicants of inorganic layered materials filled with polymers with different hydrophilicity properties.

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Label-free and culture-free microbe detection by three dimensional hot-junctions of flexible Raman-enhancing nanohybrid platelets

Abstract: A novel flexible SERS substrate with 3D hot-junction capability by employing the nanohybrids of silver nanoparticles and silicate platelets.

Cited by 31 publications

References 40 publications

Immobilization and 3D Hot-Junction Formation of Gold Nanoparticles on Two-Dimensional Silicate Nanoplatelets as Substrates for High-Efficiency Surface-Enhanced Raman Scattering Detection

Immobilization and 3D Hot-Junction Formation of Gold Nanoparticles on Two-Dimensional Silicate Nanoplatelets as Substrates for High-Efficiency Surface-Enhanced Raman Scattering Detection

Au Nanoparticles Immobilized on Honeycomb-Like Polymeric Films for Surface-Enhanced Raman Scattering (SERS) Detection

A Review on Layered Mineral Nanosheets Intercalated with Hydrophobic/Hydrophilic Polymers and Their Applications

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