Label as you fold: methyltransferase-assisted functionalization of DNA nanostructures

Abstract

The DNA methyltransferase M.TaqI is presented as a new tool to label DNA nanostructures covalently with fluorophores and azide groups.

In Situ Covalent Functionalization of DNA Origami Virus-like Particles

“…We report a scalable and efficient fabrication protocol for covalently functionalized DNA origami. This work overcomes several key technical challenges, compared with previous work, − toward fabricating and characterizing covalently functionalized DNA origami nanostructures through a postassembly functionalization workflow. First, we identified and optimized reaction protocols to achieve quantitative reaction conversions with efficient reaction stoichiometries.…”

“…Gothelf and co-workers demonstrated single-molecule orthogonal chemical reactions on origami nanostructures, and others have functionalized DNA origami using protein tags. − Andersen and co-workers used click chemistry to attach an enzyme to DNA nanostructures . Recently, strain-promoted azide–alkyne cycloaddition (SPAAC) chemistry was employed to generate superstructures from monomeric DNA origami and dense arrays of fluorophores on DNA nanostructures . These reports rely on atomic force microscopy or transmission electron microscopy (TEM) to evaluate reaction conversion.…”

“…44 Recently, strain-promoted azide− alkyne cycloaddition (SPAAC) chemistry was employed to generate superstructures from monomeric DNA origami 45 and dense arrays of fluorophores on DNA nanostructures. 46 These reports rely on atomic force microscopy or transmission electron microscopy (TEM) to evaluate reaction conversion. Both methods are semiquantitative for ensemble measurements and are not amenable to analyzing small-molecule conjugates, dense arrays, and conjugations on 3D structures.…”

Self‐Assemblies of DNA ‐ Amphiphiles Nanostructures for New Design Strategies of Varied Morphologies

“…In 2020, Heck et al. presented [67] a one‐pot enzymatic approach for grafting covalent labels onto DNA origami nanostructures along with direct labeling with fluorescent dyes. Structures with narrow intensity distributions and high fluorescence intensities are produced using this method.…”

“…Fan and colleagues created multiple-armed DNA tetrahedral nanostructures (TDNs) in 2016 for dual-modality in-vivo imaging utilising near-infrared (NIR) fluorescence and single-photon emission computed tomography (SPECT), which is used in tumor targeting. [66] In 2020, Heck et al presented [67] a one-pot enzymatic approach for grafting covalent labels onto DNA origami nanostructures along with direct labeling with fluorescent dyes. Structures with narrow intensity distributions and high fluorescence intensities are produced using this method.…”