Lab-Scale production of Bacillus atrophaeus' spores by solid state fermentation in fifferent types of bioreactors

Sella, Sandra Regina B. R.; Guizelini, Belquis P.; Vandenberghe, Luciana Porto de Souza; Medeiros, Adriane Bianchi Pedroni; Soccol, Carlos Ricardo

doi:10.1590/s1516-89132009000700021

Cited by 18 publications

(8 citation statements)

References 24 publications

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“…Spores production was conducted in trays [36,40] and packed-bed bioreactors [37,38] using different alternative substrates such as wheat bran, maize meal, rice straw and sugarcane bagasse with both fungal (Beauveria bassiana and Clonostachys roses) and bacterial (Bacillus licheniformis) strains. Sporulation reached better results in packed-bed bioreactor from 0.9 to 3.36 spores g dry matter probably due to forced aeration that is promoted in this type of equipment, which is the advantage of this model.…”

Section: Agriculture Bioproducts Produced In Ssf Bioreactorsmentioning

confidence: 99%

Solid-state fermentation technology and innovation for the production of agricultural and animal feed bioproducts

Vandenberghe

Pandey

Carvalho

et al. 2020

Syst Microbiol and Biomanuf

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It has now passed more than forty years since solid-state fermentation (SSF) research developments have gained importance for the scientific community. After so many years, numerous processes and equipment for SSF were studied and designed focusing on the production of different commercially relevant bioproducts such as enzymes, fermented food, such as Chinese daqu and koji, organic acids, pigments, phenolic compounds, aromas, biosorbents and so many others. However, no review paper has been focused yet specifically on agricultural and animal feed bioproducts obtained through SSF techniques. This review comprises the description of agricultural sub-products that have been employed in most important developed processes concerning the production of animal feed products and agricultural products such as spores, probiotics, biofungicides, bioinsecticides and other biopesticides, biofertilizers and plant growth hormones. Major designed SSF bioreactors are also described and the most important related cases of successful employment of the technique are reported. Finally, a summary of patents and innovations regarding SSF products and processes in this area is presented, showing that the main involved countries are China, South Korea, India and the USA. It is clear that the interest in this theme is increasing and that scientific and technological developments are still needed.

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Section: Agriculture Bioproducts Produced In Ssf Bioreactorsmentioning

confidence: 99%

Solid-state fermentation technology and innovation for the production of agricultural and animal feed bioproducts

Vandenberghe

Pandey

Carvalho

et al. 2020

Syst Microbiol and Biomanuf

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“…The strain was obtained from Spordex Biological Indicator Strips (Bacillus atrophaeus nrrl#4418, Steris, USA) and spores were aseptically transferred to modified soy bean casein digest broth. B. atrophaeus is a facultative anaerobic, gram-positive and rod that makes endospores (Sella et al 2009). After activation in liquid culture medium, the strain was cultivated in Trypticase Soy Broth.…”

Section: Microorganisms and Culture Mediamentioning

confidence: 99%

Survival of the Halophilic Archaeon Halovarius luteus after Desiccation, Simulated Martian UV Radiation and Vacuum in Comparison to Bacillus atrophaeus

Feshangsaz

Semsarha²,

Tackallou

et al. 2020

Orig Life Evol Biosph

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Extraterrestrial environments influence the biochemistry of organisms through a variety of factors, including high levels of radiation and vacuum, temperature extremes and a lack of water and nutrients. A wide variety of terrestrial microorganisms, including those counted amongst the most ancient inhabitants of Earth, can cope with high levels of salinity, extreme temperatures, desiccation and high levels of radiation. Key among these are the haloarchaea, considered particularly relevant for astrobiological studies due to their ability to thrive in hypersaline environments. In this study, a novel haloarchaea isolated from Urmia Salt Lake, Iran, Halovarius luteus strain DA50T, was exposed to varying levels of simulated extraterrestrial conditions and compared to that of the bacteria Bacillus atrophaeus. Bacillus atrophaeus was selected for comparison due to its well-described resistance to extreme conditions and its ability to produce strong spore structures. Thin films were produced to investigate viability without the protective influence of cell multi-layers. Late exponential phase cultures of Hvr. luteus and B. atrophaeus were placed in brine and phosphate buffered saline media, respectively. The solutions were allowed to evaporate and cells were encapsulated and exposed to radiation, desiccation and vacuum conditions, and their post-exposure viability was studied by the Most Probable Number method. The protein profile using High Performance Liquid Chromatography and Matrix Assisted Laser Desorption/Ionization bench top reflector time-of-flight are explored after vacuum and UV-radiation exposure. Results showed that the change in viability of the spore-forming bacteria B. atrophaeus was only minor whereas Hvr. luteus demonstrated a range of viability under different conditions. At the peak radiation flux of 105 J/m2 under nitrogen flow and after two weeks of desiccation, Hvr. luteus demonstrated the greatest decrease in viability. This study further expands our understanding of the boundary conditions of astrobiologically relevant organisms in the harsh space environment.

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“…The research, as reported by Frómeta et al (2020), stated that the pectin content in the coffee pulp increased by 20.5% after being treated with AIS. Another critical factor to consider in the SSF process is the sufficient water content in the medium (Sella et al, 2009). The results proved that the optimum pectinase activity of ISH16 was 0.166 U/ml when SSF was done at 90% water content in the substrate (Figure 2).…”

Section: Optimum Pectinase Productionmentioning

confidence: 88%

An Extracellular Pectinase from ISH16 Bacteria Isolated Induced by Coffee Pulp Waste Substrate

et al. 2022

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An α-1,4-glycosidic bonds galactoses pectin, mainly composed of a D-galacturonic acid chain, are important biomaterial widely used in industries. Utilizing this material, a bioprocess, including the biocatalysis pectinase, is often needed. Pectinase production was optimized in 7 days SSF at 37°C, and the pectinase activities were daily measured by the method of Somogy-Nelson. The optimum pectinase production was 0.166 U/ml on the fourth day SSF. Purification using open column ion exchange chromatography DEAE cellulose DE-52 resulted in 1030.9 folds of pectinase purity with a yield of 25.9%. The enzyme was at optimal activity at pH six and attended stable in the pH range of 5.5-8, while optimal activity at a temperature of 50°C and was stable in the range of 30-45°C. The pectinase activity increased by 120% with the addition of 10 mM Mg2+, and 95% retained when 10 mM Ca2+ was added. The presence of 10 mM Na+, K+, and Fe2+ resulted in a slight effect of activity at 85%, 83%, and 78%. However, it was strongly inhibited by 10 mM Al3+ and retained 25%. Based on the results above, the microbial utilization of coffee pulp waste by ISH16 bacteria pectinolytic is one opportunity to produce valuable pectinase with low-cost production, so comprehensive examination in large-scale production is needed too. In this paper, all research detail steps were described.

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Lab-Scale production of Bacillus atrophaeus' spores by solid state fermentation in fifferent types of bioreactors

Abstract: ABSTRACT

Cited by 18 publications

References 24 publications

Solid-state fermentation technology and innovation for the production of agricultural and animal feed bioproducts

Solid-state fermentation technology and innovation for the production of agricultural and animal feed bioproducts

Survival of the Halophilic Archaeon Halovarius luteus after Desiccation, Simulated Martian UV Radiation and Vacuum in Comparison to Bacillus atrophaeus

An Extracellular Pectinase from ISH16 Bacteria Isolated Induced by Coffee Pulp Waste Substrate

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