Lab on Fiber Technology for biological sensing applications

Vaiano, Patrizio; Carotenuto, Benito; Pisco, Marco; Ricciardi, Armando; Quero, Giuseppe; Consales, M.; Crescitelli, A.; Esposito, E.; Cusano, A.

doi:10.1002/lpor.201600111

Cited by 227 publications

(156 citation statements)

References 254 publications

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“…Even though the development of OFT is a growing field, only a limited number of options are available for simultaneous cell trapping and sensing 1,2,5,15 . Plasmonic fiber tweezers are good candidates for such an hybrid task because, beyond trapping, they are sensitive to tiny changes of the surrounding environmental refractive index and can then provide some additional information about their target 16 .…”

mentioning

confidence: 99%

iLoF: An intelligent Lab on Fiber Approach for Human Cancer Single-Cell Type Identification

Paiva,

Jorge,

Ribeiro

et al. 2020

Sci Rep

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With the advent of personalized medicine, there is a movement to develop "smaller" and "smarter" microdevices that are able to distinguish similar cancer subtypes. Tumor cells display major differences when compared to their natural counterparts, due to alterations in fundamental cellular processes such as glycosylation. Glycans are involved in tumor cell biology and they have been considered to be suitable cancer biomarkers. Thus, more selective cancer screening assays can be developed through the detection of specific altered glycans on the surface of circulating cancer cells. Currently, this is only possible through time-consuming assays. In this work, we propose the "intelligent" Lab on Fiber (iLof) device, that has a high-resolution, and which is a fast and portable method for tumor single-cell type identification and isolation. We apply an Artificial Intelligence approach to the back-scattered signal arising from a trapped cell by a micro-lensed optical fiber. As a proof of concept, we show that iLof is able to discriminate two human cancer cell models sharing the same genetic background but displaying a different surface glycosylation profile with an accuracy above 90% and a speed rate of 2.3 seconds. We envision the incorporation of the iLoF in an easy-to-operate microchip for cancer identification, which would allow further biological characterization of the captured circulating live cells.Recent research trends on healthcare point out to the movement to develop "smart" micro-tools to allow better personalized diagnostic and therapeutic approaches 1-3 . Considering that current medicine and biotechnology attempts are converging to novel methodologies at the micro (e.g., cancer cells detection) and nano scales (e.g., cancer-related extracellular vesicles detection), an effort towards the development of these "intelligent" microdevices with multifunctionalities is required 3 . In this regard, optical fiber tools -for example, Optical Fiber Tweezers (OFT) 1,4,5 -have emerged as suitable candidates thanks to their flexibility, small size and chemical inertness, which contributes to the advent of a novel concept of "Lab on Fiber" (LoF) devices 2 . The fruitful application of these optical-based microdevices in cancer screening has been envisioned as straightforward 1,2 . However, the high degree of heterogeneity among cancer subtypes must be taken into consideration 6,7 . This heterogeneity is mainly due to both cellular and microenvironmental factors, such as alterations in cellular glycosylation 8 . In particular, the selective detection of specific cancer-associated glycoforms expressed at the surface of circulating cancer cells could increase the specificity of cancer biomarker assays and therapeutic approaches 8-10 . In fact, tumor heterogeneity is considered to be a major barrier to an effective cancer diagnosis and treatment 6,7 . Recent evidence has shown that glycans can determine the acquisition of certain cellular features controlling tumor growth and progression 8,11,12 . For example, shorter trun...

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mentioning

confidence: 99%

iLoF: An intelligent Lab on Fiber Approach for Human Cancer Single-Cell Type Identification

Paiva,

Jorge,

Ribeiro

et al. 2020

Sci Rep

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“…For further details regarding the electromagnetic principles behind PC defects, readers are referred to [51]. Demonstrated applications include refractive index sensing, optical communication, protein detection, optical trapping, and single nanoparticle detection [52][53][54][55][56][57][58].…”

Section: Photonic Crystal Cavitiesmentioning

confidence: 99%

“…The ability of optical fibers to conduct light from a sensing region to a remote location, makes them an ideal platform to perform measurements in highly confined spaces. "Lab on a Fiber" [54] technology is aimed at developing innovative types of optical fiber probes with extraordinary features in terms of miniaturization levels and functionalities, especially ones exploitable for biological sensing. In particular, we wish to highlight recent work in which a PC is produced upon the tip of an optical fiber.…”

Section: Point-like Defectsmentioning

confidence: 99%

Detection and Digital Resolution Counting of Nanoparticles with Optical Resonators and Applications in Biosensing

Aguirre

Long

et al. 2018

Chemosensors

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Abstract:The interaction between nanoparticles and the electromagnetic fields associated with optical nanostructures enables sensing with single-nanoparticle limits of detection and digital resolution counting of captured nanoparticles through their intrinsic dielectric permittivity, absorption, and scattering. This paper will review the fundamental sensing methods, device structures, and detection instruments that have demonstrated the capability to observe the binding and interaction of nanoparticles at the single-unit level, where the nanoparticles are comprised of biomaterial (in the case of a virus or liposome), metal (plasmonic and magnetic nanomaterials), or inorganic dielectric material (such as TiO 2 or SiN). We classify sensing approaches based upon their ability to observe single-nanoparticle attachment/detachment events that occur in a specific location, versus approaches that are capable of generating images of nanoparticle attachment on a nanostructured surface. We describe applications that include study of biomolecular interactions, viral load monitoring, and enzyme-free detection of biomolecules in a test sample in the context of in vitro diagnostics.

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“…Among the LOF technology platforms, “Lab-on-Tip” devices are particularly attractive because of the simplicity of the integration of the sensing material on the fiber tip [4]. The application of LOF approach to temperature sensing has been demonstrated using several measuring principles, like for example Fabry-Perot interferometers [5,6], Fiber Bragg gratings [7,8] or surface plasmon resonance [9,10].…”

Section: Introductionmentioning

confidence: 99%

Mn4+-Doped Magnesium Titanate—A Promising Phosphor for Self-Referenced Optical Temperature Sensing

Venturini

Baumgärtner

Borisov

2018

Sensors

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Phosphors based on magnesium titanate activated with Mn4+ ions are of great interest because, when excited with blue light, they display a strong red-emitting luminescence. They are characterized by a luminescence decay which is strongly temperature dependent in the range from −50 ∘C to 150 ∘C, making these materials very promising for temperature sensing in the biochemical field. In this work, the optical and thermal properties of the luminescence of Mg2TiO4 are investigated for different Mn4+ doping concentrations. The potential of this material for temperature sensing is demonstrated by fabricating a fiber optic temperature microsensor and by comparing its performance against a standard resistance thermometer. The response of the fiber optic sensor is exceptionally fast, with a response time below 1 s in the liquid phase and below 1.1 s in the gas phase.

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Lab on Fiber Technology for biological sensing applications

Cited by 227 publications

References 254 publications

iLoF: An intelligent Lab on Fiber Approach for Human Cancer Single-Cell Type Identification

iLoF: An intelligent Lab on Fiber Approach for Human Cancer Single-Cell Type Identification

Detection and Digital Resolution Counting of Nanoparticles with Optical Resonators and Applications in Biosensing

Mn4+-Doped Magnesium Titanate—A Promising Phosphor for Self-Referenced Optical Temperature Sensing

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