Lab-on-a-Molecule Probe: Multitarget Detection of Five Aromatic Pesticides Using a Supramolecular Probe under Single Wavelength Excitation

Abstract

In order to prevent and control the effects of pesticide residues on human health and the ecological environment, the rapid, highly sensitive, and selective detection of multiple pesticide residues has become an urgent problem to be solved. Herein, a lab-on-a-molecule probe based on a host−guest complex (ThT@Q[8] probe) has been developed to simultaneously analyze multiple aromatic pesticides under single wavelength excitation, such as fuberidazole, thiabendazole, carbendazim, thidiazuron, and tricyclazole. The fluorescence titration spectra of the ThT@Q[8] probe with the five pesticides mentioned above showed that the fluorescence intensity exhibited a good linear correlation with the pesticide concentration and the limit of detection was as low as 10 −7 M. Because the ThT@Q[8] probe exhibits diverse fluorescence color changes to the five pesticides studied under a 365 nm ultraviolet lamp, we fabricated a single probe used to detect multiple analytes in the RGB triple channel by extracting the RGB variations. Principal component analysis and linear discriminant analysis proved that the ThT@Q[8] probe can recognize and distinguish five pesticides and can be applied at different concentrations. In real samples, the ThT@Q[8] probe recognized and distinguished five pesticides in tap water and Huaxi River water. The 1 H NMR spectra results proved that a charge-transfer complex of ThT and pesticides in the Q[8] cavity may be formed. Moreover, we selected a test strip as a carrier to detect pesticides. The results indicate it can be used to quickly and conveniently detect different pesticides due to the rapid color change. Besides, the ThT@Q[8] probe has good cell permeability and can be used to detect pesticide residues in living cells. This work has laid the foundation for the qualitative and quantitative multitarget detection of pesticide residues.

Production of Antibacterial Questiomycin A in Metabolically Engineered Pseudomonas chlororaphis HT66

“…Pesticides are indispensable in agriculture and play a critical role in fulfilling rising demands for food productivity and quality. − Unfortunately, environmental pollution caused by the widespread use of agrochemicals cannot be ignored. , Biological control has become a more acceptable technique to control plant diseases or pests and overcome environmental challenges. − Pseudomonas species can produce a wide range of novel bioactive metabolites and play an important role in biological control. − …”

A supramolecular fluorescence array sensor for toxic heavy metal ion detection in environmental water and rice seedling extracts

“…[24][25][26][27][28][29] Therefore, a sensing array can be designed based on the cross-reactivity of the interaction between the fluorescent probe derived from cucurbit[n]uril and the analyte. The use of supramolecular array sensors based on cucurbit[n]uril to detect and recognize multi-target analytes, such as bioactive metabolites, [30][31][32] environmental pollutants, 33 and pesticide residues, 34 has received considerable attention.…”

Portable Sensor Array for On-Site Detection and Discrimination of Pesticides and Herbicides Using Multivariate Analysis

“…9,10 The extensive utilization of pesticides and herbicides is causing severe issues with environment and food safety, and thus, it is crucial to develop reliable and robust techniques for pesticide and herbicide detection. 11 Several approaches have been established for the qualitative and quantitative examination of pesticides and herbicides, which comprises chromatographic methods, 12,13 electrochemical methods, 14,15 (SERS), 16 enzyme-linked immunosorbent assays (ELISAs), 17 aptamers, 18 etc. However, these technologies have some drawbacks such as time-consuming measurements, tedious sample preparation, and expense, which makes them impractical for on-site investigation.…”