Lab-on-a-chip platform for high throughput drug discovery with DNA-encoded chemical libraries

Recent advances in DNA-encoded dynamic libraries

“…Collectively, these studies have nicely shown that DNA encoding can indeed be used to prepare, process, and select large dynamic libraries against proteins. In addition, EDCCL has been adapted to an automated microfluidic platform with improved efficiency, 124 and the technology has also been commercialized and is being sold as a selection kit (DyNAbind ® ) for fragment discovery. 12 For DCL selections, after the equilibrium is shifted to a new state induced by the target, an equilibrium locking procedure is carried out to stop the dynamic exchange, so that the "locked" equilibrium may be compared with the one without target addition (or other controls) for hit identification.…”

“…Collectively, these studies have nicely shown that DNA encoding can indeed be used to prepare, process, and select large dynamic libraries against proteins. In addition, the EDCCL has been adapted to an automated microfluidic platform with improved efficiency, 124 and the technology has also been commercialized and is being sold as a selection kit (DyNAbind®) for fragment discovery. 12…”

Converting Double-Stranded DNA-Encoded Libraries (DELs) to Single-Stranded Libraries for More Versatile Selections

“…In fact, today, the majority of DELs are encoded with dsDNA tags. Although having been used in early studies, , DELs encoded with single-stranded DNAs (ssDELs) are less abundant and they are often prepared with special encoding methods, such as DNA-templated synthesis (DTS), DNA routing, and DNA-directed assembly. , ssDNAs can also be used to assemble the encoded self-assembling chemical (ESAC) libraries − and DNA-encoded dynamic libraries. − Moreover, ssDELs are amendable to a number of selection methods suitable for nonimmobilized proteins, such as the interaction determination using unpurified proteins (IDUP), , the cross-linking-based methods, − and the selections against membrane proteins on/inside live cells (Figure c). , In these methods, the ssDNA tag provides a site for hybridization with an additional DNA to covalently connect with the target upon binding. These methods may expand the target scope of DELs to more complex targets in a more biologically relevant environment. , In addition, a recent report showed that ssDELs had higher enrichment folds than dsDELs, especially for low- to moderate-affinity binders .…”

Hematocrit-insensitive Absolute Blood Flow Rate Measurement in 0.5-mm-diameter Flow Channel Using MEMS-based Laser Doppler Velocimeter with Signal Modification for Detecting Beat Frequency from Broad Power Spectrum

“…Microf luidic device technology is becoming increasingly important in the biomedical field. (1) To date, a variety of microf luidic devices have been researched and developed toward realizing the lab-on-a-chip (LoC) and micro-total analysis system (µ-TAS) (2)(3)(4)(5)(6) for various analyses and applications, such as liquid chromatography, (7) protein separation, (8) rare metal extraction, (9) Raman spectroscopy, (10) DNA analysis, (11)(12)(13)(14) drug discovery, (15) electrotaxis study, (16) cancer biomarker detection, (17) and hormone detection. (18) Many of these applications require precise control of the f low rate.…”