Lab-on-a-chip flow cytometer employing color-space-time coding

Cho, Sung Hwan; Qiao, Wen; Tsai, Frank S.; Yamashita, Kenichi; Lo, Yu‐Hwa

doi:10.1063/1.3481695

Cited by 23 publications

(21 citation statements)

References 10 publications

(9 reference statements)

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“…Other optical developments appear such as colorspace-time (COST) coding, which is a new way to detect multiple fluorescent wavelengths using a single photodetector (Cho et al 2010b). This method of discriminating multiple fluorescent colors with a single photomultiplier holds great promise to significantly reduce the cost and size of the total system.…”

Section: Perspectivesmentioning

confidence: 99%

Flow cytometry: retrospective, fundamentals and recent instrumentation

et al. 2012

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Flow cytometry is a complete technology given to biologists to study cellular populations with high precision. This technology elegantly combines sample dimension, data acquisition speed, precision and measurement multiplicity. Beyond the statistical aspect, flow cytometry offers the possibility to physically separate sub-populations. These performances come from the common endeavor of physicists, biophysicists, biologists and computer engineers, who succeeded, by providing new concepts, to bring flow cytometry to current maturity. The aim of this paper is to present a complete retrospective of the technique and remind flow cytometry fundamentals before focusing on recent commercial instrumentation.

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Section: Perspectivesmentioning

confidence: 99%

Flow cytometry: retrospective, fundamentals and recent instrumentation

et al. 2012

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“…III B͒, a novel way to detect multiple fluorescent wavelengths using a single photodetector. 62 Here, the focus is on minimizing the size and cost of not just the chip, but also the surrounding optical detection system as well. This method of discriminating multiple fluorescent colors with a single PMT, pioneered in our laboratory, holds great promise to significantly reduce the cost and size of the total system.…”

Section: -9mentioning

confidence: 99%

“…62 The technology mimics the working principles of human eyes, which can distinguish more than 1000 colors using only three types of photoreceptor cells. 65 In the somewhat analogous COST coding method, a fluorescence emission signal is encoded into a time-dependent signal as each fluorescently tagged cell passes by an on-chip spatial and color filter waveguide array.…”

Section: B Multicolor Detection Employing Cost Coding Technologymentioning

confidence: 99%

Review Article: Recent advancements in optofluidic flow cytometer

Cho¹,

Godin²,

Chen³

et al. 2010

Biomicrofluidics

Self Cite

139

105

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There is an increasing need to develop optofluidic flow cytometers. Optofluidics, where optics and microfluidics work together to create novel functionalities on a small chip, holds great promise for lab-on-a-chip flow cytometry. The development of a low-cost, compact, handheld flow cytometer and microfluorescence-activated cell sorter system could have a significant impact on the field of point-of-care diagnostics, improving health care in, for example, underserved areas of Africa and Asia, that struggle with epidemics such as HIV/AIDS. In this paper, we review recent advancements in microfluidics, on-chip optics, novel detection architectures, and integrated sorting mechanisms.

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“…Not just the optically passive devices such as polymer optical fibers and plastic waveguides, the active devices having functionalities of the light emission or optical amplification also gather much attention, because the various luminescent materials can be easily doped into the polymer materials. These devices can be easily operated by the optical pumping, and thus would be expected as an integrated light source in compact sensing systems based on the lab-on-a-chip concept [1][2][3][4]. For the sensing purpose, the integrated light source devices would be required to have particular spectral features, e.g.…”

Section: Introductionmentioning

confidence: 99%

Integrated light sources based on self-formed polymer waveguide doped with active medium

Sugimoto

Kawaguchi

Yamashita

2012

Organic Photonics V

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The polymer-based photonic devices have promise as optical components integrated in convenient microchip systems because of ease in the deposition and microfabrication of polymer materials. Furthermore, since the polymers can be easily doped with luminescent materials such as organic dyes, the organic light source devices are also considerable as the integrated optical components. Especially the self-formation method of the fiber type waveguide using the dyedoped photopolymers, called as self-written active (SWA) waveguide technique, brings a simple fabrication scheme of the light sources integrated in the polymer photonic circuit. It has been demonstrated that an in-line optical amplifier and a Fabry-Perot type lasing cavity can be obtained by using the SWA waveguide technique. In this study we have employed this technique to fabricate an incoherent light source with a wideband emission. A serially cascaded SWA waveguide doped with the different organic dyes could be fabricated with a single exposure process. The cascaded SWA waveguide could act as an incoherent light source under UV light irradiation. This device can be expected as a probing light source for the label-free microchip system, which would be available for the biological sensing, environmental measurements, and medical diagnosis.

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Lab-on-a-chip flow cytometer employing color-space-time coding

Cited by 23 publications

References 10 publications

Flow cytometry: retrospective, fundamentals and recent instrumentation

Flow cytometry: retrospective, fundamentals and recent instrumentation

Review Article: Recent advancements in optofluidic flow cytometer

Integrated light sources based on self-formed polymer waveguide doped with active medium

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