Lab on a chip automates in vitro cell culturing

Perozziello, Gerardo; MøLlenbach, Jacob; Laursen, Steen; Fabrizio, E. Di; Gernaey, Krist V.; Krühne, Ulrich

doi:10.1016/j.mee.2012.07.027

Cited by 20 publications

(10 citation statements)

References 15 publications

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“…Microfluidic devices have been developed and exploited from several research groups as bioanalytical tools to give several advantages over conventional bioanalytical technologies. In fact, these devices guarantee high portability, simplified pretreatment protocols, accurate handling and low consumption of samples and reagents, as well as high resolution analysis and low production costs [24]. There are many examples in literature of microfluidic devices used for analytical purposes [25,26].…”

Section: Raman Spectroscopy Compatible Microfluidic Devices For Cell mentioning

confidence: 99%

Nanoplasmonic and Microfluidic Devices for Biological Sensing

Perozziello

Giugni

Allione

et al. 2017

NATO Science for Peace and Security Series B: Physics and Biophysics

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In this chapter we report about recent advances on the development and application of 2D and 3D plasmonic nanostructures used for sensing of biological samples by Raman spectroscopy at unprecedented resolution of analysis. Besides, we explain how the integration of these nanodevices in a microfluidic apparatus can simplify the analysis of biological samples. In the first part we introduce and motivate the convenience of using nanoplasmonic enhancers and Raman spectroscopy for biological sensing, describing the phenomena and the current approaches to fabricate nanoplasmonic structures. In the second part, we explain how specific multi-element devices produce the optimal enhancement of the Raman scattering. We report cases where biological sensing of DNA was performed at few molecules level with nanometer spatial resolutions. Finally, we show an example of microfluidic device integrating plasmonic nanodevices to sort and drive biological samples, like living cells, towards the optical probe in order to obtain optimal conditions of analysis.

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Section: Raman Spectroscopy Compatible Microfluidic Devices For Cell mentioning

confidence: 99%

Nanoplasmonic and Microfluidic Devices for Biological Sensing

Perozziello

Giugni

Allione

et al. 2017

NATO Science for Peace and Security Series B: Physics and Biophysics

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“…Microfluidic systems provide an excellent control of microenvironments [2][3][4], which is useful for high-performance cellular screenings [5][6][7][8], due to a high level of functional elements [9][10][11] and sensor integration [12][13][14][15][16], and for generating different types of gradients [17][18][19][20]. Moreover, microfluidic platforms offer the possibility to dynamically and automatically modify the microenvironment in different ways [21][22][23] for several applications [24][25][26][27].…”

Section: Introductionmentioning

confidence: 99%

A Disposable Passive Microfluidic Device for Cell Culturing

et al. 2020

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In this work, a disposable passive microfluidic device for cell culturing that does not require any additional/external pressure sources is introduced. By regulating the height of fluidic columns and the aperture and closure of the source wells, the device can provide different media and/or drug flows, thereby allowing different flow patterns with respect to time. The device is made of two Polymethylmethacrylate (PMMA) layers fabricated by micro-milling and solvent assisted bonding and allows us to ensure a flow rate of 18.6 μL/h - 7%/day, due to a decrease of the fluid height while the liquid is driven from the reservoirs into the channels. Simulations and experiments were conducted to characterize flows and diffusion in the culture chamber. Melanoma tumor cells were used to test the device and carry out cell culturing experiments for 48 h. Moreover, HeLa, Jurkat, A549 and HEK293T cell lines were cultivated successfully inside the microfluidic device for 72 h.

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“…To closely mimic the microenvironment of cells, the design of MBR can be tailored for specific applications, including cytotoxicity testing and drug development . In a microfluidic setup, they are also used as cell culturing and analytical devices, in clinical embryology or tissue engineering . Nowadays, advancing developments in manufacturing techniques enable increasingly smaller reactor setups to optimally use the advantages of miniaturization …”

Section: Introductionmentioning

confidence: 99%

“…11,12 In a microfluidic setup, they are also used as cell culturing and analytical devices, in clinical embryology or tissue engineering. 13,14 Nowadays, advancing developments in manufacturing techniques enable increasingly smaller reactor setups to optimally use the advantages of miniaturization. 15 To ensure scalability during bioprocess development from microscale to lab-or pilot-scale, heat, and mass transfer have to be comparable in both systems.…”

mentioning

confidence: 99%

Novel electrodynamic oscillation technique enables enhanced mass transfer and mixing for cultivation in micro‐bioreactor

Frey

Vorländer

Rasch

et al. 2019

Biotechnology Progress

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Micro‐bioreactors (MBRs) have become an indispensable part for modern bioprocess development enabling automated experiments in parallel while reducing material cost. Novel developments aim to further intensify the advantages as dimensions are being reduced. However, one factor hindering the scale‐down of cultivation systems is to provide adequate mixing and mass transfer. Here, vertical oscillation is demonstrated as an effective method for mixing of MBRs with a reaction volume of 20 μL providing adequate mass transfer. Electrodynamic exciters are used to transduce kinetic energy onto the cultivation broth avoiding additional moving parts inside the applied model MBR. The induced vertical vibration leads to oscillation of the liquid surface corresponding to the frequency and displacement. On this basis, the resonance frequency of the fluid was identified as the most decisive factor for mixing performance. Applying this vertical oscillation method outstanding mixing times below 1 s and exceptionally high oxygen transport with volumetric mass transfer coefficients (kLa) above 1,000/hr can be successfully achieved and controlled. To evaluate the applicability of this vertical oscillation mixing for low volume MBR systems, cultivations of Escherichia coli BL21 as proof‐of‐concept were performed. The dissolved oxygen was successfully online monitored to assure any avoidance of oxygen limitations during the cultivation. The here presented data illustrate the high potential of the vertical oscillation technique as a flexible measure to adapt mixing times and oxygen transfer according to experimental demands. Thus, the mixing technique is a promising tool for various biological and chemical micro‐scale applications still enabling adequate mass transfer.

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Lab on a chip automates in vitro cell culturing

Cited by 20 publications

References 15 publications

Nanoplasmonic and Microfluidic Devices for Biological Sensing

Nanoplasmonic and Microfluidic Devices for Biological Sensing

A Disposable Passive Microfluidic Device for Cell Culturing

Novel electrodynamic oscillation technique enables enhanced mass transfer and mixing for cultivation in micro‐bioreactor

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