Lab on a Biomembrane: Rapid prototyping and manipulation of 2D fluidic lipid bilayer circuits

Ainla, Alar; Gözen, İrep; Hakonen, Bodil; Jesorka, Aldo

doi:10.1038/srep02743

Cited by 27 publications

(43 citation statements)

References 33 publications

(38 reference statements)

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“…SLBs are common model systems of the cell membrane and consists of a lipid bilayer adsorbed on, typically a glass surface. [8][9][10] Here different macromolecules including lipids with modified head groups, [11][12][13][14][15] lipid vesicles, 16 bound proteins, 11,13,17 and DNA 18,19 have been transported in the direction of the flow, resulting in a varying concentration of the studied molecules over the lipid bilayer. Figure 1A shows a schematic illustration of an SLB with anchored macromolecules protruding from the SLB under the influence of an external liquid flow.…”

Section: Introductionmentioning

confidence: 99%

Hydrodynamic Forces on Macromolecules Protruding from Lipid Bilayers Due to External Liquid Flows

Jönsson

2015

Langmuir

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It has previously been observed that an externally applied hydrodynamic shear flow above a fluid lipid bilayer can change the local concentration of macromolecules that are associated with the lipid bilayer. The external liquid flow results in a hydrodynamic force on molecules protruding from the lipid bilayer, causing them to move in the direction of the flow.However, there has been no quantitative study about the magnitude of these forces. We here use finite element simulations to investigate how the magnitude of the external hydrodynamic forces varies with the size and shape of the studied macromolecule. The simulations show that the hydrodynamic force is proportional to the effective hydrodynamic area of the studied molecule, A hydro , multiplied by the mean hydrodynamic shear stress acting on the membrane surface,  hydro .The parameter A hydro depends on the size and shape of the studied macromolecule above the lipid bilayer and scales with the cross-sectional area of the molecule. We also investigate how hydrodynamic shielding from other surrounding macromolecules decreases A hydro when the surface coverage of the shielding macromolecules increases. Experiments where the protein streptavidin is anchored to a supported lipid bilayer on the floor of a microfluidic channel were finally performed at three different surface concentrations, Φ = 1%, 6%, and 10%, where the protein is being moved relative to the lipid bilayer by a liquid flow through the channel. From photobleaching measurements of fluorescently labeled streptavidin we found the experimental drift data to be within good accuracy of the simulated results, less than 12% difference, indicating the validity of the results obtained from the simulations. In addition to giving a deeper insight into how a liquid flow can affect membrane-associated molecules in a lipid bilayer, we also see an interesting potential of using hydrodynamic flow experiments together with the obtained results to study the size and the intermolecular forces between macromolecules in membranes and lipid bilayers.3

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Section: Introductionmentioning

confidence: 99%

Hydrodynamic Forces on Macromolecules Protruding from Lipid Bilayers Due to External Liquid Flows

Jönsson

2015

Langmuir

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“…A laser scanning confocal microscope (Leica TCS SP8, Leica Microsystems GmbH, Wetzlar, Germany) was used. The supported lipid bilayers were formed in situ by transforming SUVs on a glass substrate (WillCo Wells B.V. Amsterdam, NL), using an open-space microfluidic multichannel pipette 17 (Fluicell AB, Sweden). For deposition of the lipids, the microfluidic pipette was positioned using a 3-axis water hydraulic micromanipulator (Narishige, Japan) 10-20 mm above the surface and the recirculation of SUVs (0.1 mg ml À1 ) was initiated ( Fig.…”

Section: Lipid Film Formation and Polymer Exposurementioning

confidence: 99%

“…1a). This leads to the adhesion of SUVs onto the solid surface, rupturing and eventual merging of the individual ruptured lipid patches into a circular homogeneous planar bilayer 17 (Fig. 1a).…”

Section: Lipid Film Formation and Polymer Exposurementioning

confidence: 99%

Styrene maleic acid copolymer induces pores in biomembranes

et al. 2019

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“…(1,2-bis (o-aminophenoxy) ethane-N,N,N′,N′-tetraacetic acid (BAPTA) and Ethylenediaminetetraacetic acid (EDTA) (18,20) were used in the experiments, and the vesicles with reduced pinning due to Ca 2+ depletion were easily pulled into the pipette. During collection, some of the vesicles which were lifted by the aspiration force, remain connected to the lipid patch through a nanotube ( Fig.…”

Section: Separation and Migration Of Vesiclesmentioning

confidence: 99%

A nanotube-mediated path to protocell formation

Köksal

Liese

Kantarci

et al. 2018

Preprint

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Cellular compartments are membrane-enclosed, spatially distinct microenvironments which confine and protect biochemical reactions in the biological cell. On the early Earth, the autonomous formation of compartments is thought to have led to the encapsulation of nucleotides, thereby satisfying a starting condition for the emergence of life. Recently, surfaces have come into focus as potential platforms for the self-assembly of prebiotic compartments, as significantly enhanced vesicle formation was reported in the presence of solid interfaces. The detailed mechanism of such formation at the mesoscale is still under discussion. We report here on the spontaneous transformation of solid surface-adhered lipid deposits to unilamellar membrane compartments through a straightforward sequence of topological changes, proceeding via a network of interconnected lipid nanotubes. We show that this transformation is entirely driven by surface-free energy minimization and does not require hydrolysis of organic molecules, or external stimuli such as electrical currents or mechanical agitation. The vesicular structures take up and encapsulate their external environment during formation, and can subsequently separate and migrate upon exposure to hydrodynamic flow. This may link, for the first time, the self-directed transition from weakly organized bioamphiphile assemblies on solid surfaces to protocells with secluded internal contents. protocell | biomembrane | lipid nanotube | origin of life SignificanceThe nature of the physical and chemical mechanisms behind the formation, growth and division of the earliest protocells is among the key questions concerning the origin of life. Establishing a simple pathway for the assembly of protocell structures from the primordial soup is a particular challenge. Emerging evidence supporting the assumption that solid surfaces have a governing role in protocell formation has recently expanded the scope, and created new inspiration for investigation. By presenting a physical path from self-assembled amphiphile-based membranes on solid surfaces to spherical single-membrane compartments via a consistent sequence of transformations, solely driven by the materials properties of the interfaces, a direct link between the presence of functional biomolecules and the development of protocells can be established.

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Lab on a Biomembrane: Rapid prototyping and manipulation of 2D fluidic lipid bilayer circuits

Cited by 27 publications

References 33 publications

Hydrodynamic Forces on Macromolecules Protruding from Lipid Bilayers Due to External Liquid Flows

Hydrodynamic Forces on Macromolecules Protruding from Lipid Bilayers Due to External Liquid Flows

Styrene maleic acid copolymer induces pores in biomembranes

A nanotube-mediated path to protocell formation

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