Lab-in-cell based on spontaneous amino-yne click polymerization

Hu, Rong; Xu, Chen; Zhou, Taotao; Si, Han; He, Beihai; Kwok, Ryan T. K.; Qin, Anjun; Tang, Ben Zhong

doi:10.1007/s11426-019-9517-9

Cited by 64 publications

(45 citation statements)

References 32 publications

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“…Taking advantage of its high efficiency and fantastic spontaneity, this amino-yne click polymerization reaction was successfully developed into a fancy intracellular polymerization reaction by the use of aroylacetylene instead of propiolate as the monomer. 95 Interestingly, turn-on fluorescent imaging and in situ killing of cells could be realized by this in vivo amino-yne polymerization reaction. In spite of its several advantages, such propiolate-based spontaneous amino-yne click polymerization still has some shortcomings.…”

Section: Alkyne-based Click Polymerizationmentioning

confidence: 99%

Advanced functional polymer materials

Wang¹,

Amin²,

An³

et al. 2020

Mater. Chem. Front.

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137

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Section: Alkyne-based Click Polymerizationmentioning

confidence: 99%

Advanced functional polymer materials

Wang¹,

Amin²,

An³

et al. 2020

Mater. Chem. Front.

Self Cite

137

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“…[67] It has been demonstrated that in situ polymerization in biological systems can be achieved using thermal initiation and enzymatic approaches. [68][69][70] As an alternative methodology, photopolymerization offers a key advantage in spatio-temporal control, allowing for external manipulation of these strategies. [71,72] Moving beyond encapsulating cells within a matrix, directing polymerization onto key components of a cell provides a unique opportunity to modulate cell properties using synthetic materials.…”

Section: Photopolymerizationmentioning

confidence: 99%

Shining a Light on Bioorthogonal Photochemistry for Polymer Science

Boase

2020

Macromol. Rapid Commun.

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Bioorthogonal chemistry is revolutionizing the fields of biological chemistry and nanomedicine, providing tools to actively probe and perturb native biochemical processes. Photochemistry provides the opportunity to actively and non‐invasively control bioorthogonal reactions, providing sophisticated optochemical tools. Despite the opportunities in bioorthogonal photochemistry, there remain many significant challenges to the clinical translation of current research. This review aims to provide an overview of these challenges and highlight recent examples from the literature that are providing revolutionary solutions to overcoming these barriers. It will highlight new photochemical systems that can be triggered by near infrared light in aqueous solutions and have been demonstrated to function in complex biological systems, including in living animals. It will cover diverse classes of photochemical reactions including photopolymerization, uncaging, conjugation, and photoswitching. The discussion will detail how new approaches are being integrated into polymers or highlight unexploited opportunities. This review intends to showcase how the unique synergy of bioorthogonal photochemistry and polymer science provides vast opportunities in the fields of biomaterials, nanomedicine, and theranostics. This will hopefully provide inspiration to material scientists to integrate bioorthogonal photochemistry into new adaptable materials and ensure translation to solve clinical challenges.

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“…[28][29][30][31][32] More recently, the aminoyne click reaction was proved to hold great potential for bioconjugation applications. 33,34 Attracted by this highly efficient spontaneous amino-yne click reaction, herein, we applied it in functionalizing surfaces and sequentially immobilizing native bioconjugates for the rst time. Excitingly, the amino group coated glass surfaces could be successfully functionalized with ester activated ethynyl groups through the catalyst-free aminoyne click reaction, which could be further modied with organic dyes, native proteins and cells in one step (Fig.…”

Section: Introductionmentioning

confidence: 99%