To improve survival rates of vitrified pig oocytes, the treatment of cytoskeletal
stabilizer on an appropriate time is one of the possible approaches. However,
the exact treatment timing and effect of cytoskeletal stabilizer such as
cytochalasin B (CB) is not well known during oocyte vitrification procedures.
Thus, the present study was conducted to determine optimal treatment timing of
CB during vitrification and warming procedures. In experiment 1, the survival
rates of the post-warming pig oocytes were analyzed by fluorescein diacetate
(FDA) assays with 4 classifications. In results, post-warming oocytes showed
significantly (p<0.05) decreased number of alive oocytes
(31.8% vs. 86.4%) compared to fresh control. In detail, the significant
difference (p<0.05) was found only in strong
fluorescence (18.2% vs. 70.5%) not in intermediate fluorescence groups (13.6%
vs. 15.9%). In experiment 2, CB was treated before (CB-Vitri) and after
(Vitri-CB) vitrification. In results, group of Vitri-CB showed significantly
(p<0.05) higher (91.6%) survival rates compared to
group of CB-Vitri (83.7%), significantly (p<0.05) and
comparable with group of Vitri Control (88.7%) by morphological inspection. In
FDA assay results, group of Vitri-CB showed significantly
(p<0.05) higher (44.2%) survival rates compared to
groups of CB-Vitri (36.7%) and Vitri Control (35.1%). In conclusion, the
increased survival rates of post-warming pig oocyte treated with Vitri-CB method
are firstly described here. The main finding of present study is that the CB
treatment during recovery could be helpful to refresh the post-warming pig
oocyte resulting its improved survival rates.