La trame protidique des nacres

Principles of demineralization: Modern strategies for the isolation of organic frameworks

“…Decalcification of the mother-of-pearl (nacre), isolation of organic components and discovery of stratified membranes of conchiolin Grogoire et al (1949Grogoire et al ( , 1950Grogoire et al ( , 1954Grogoire et al ( , 1955; Gregoire (1957Gregoire ( , 1959Gregoire ( ) 1949 Demineralization of enamel and isolation of eukeratin Block et al (1949Block et al ( ) 1950 Demineralization and classification of diseases in bones Haldeman (1950Haldeman ( ) 1950 Bacterial chemistry of dental plaques Stralfors (1950Stralfors ( ) 1951 EDTA (Versene) as organic chelating agent for demineralization of hard tissues ; Sreebny and ; Sreebny (1953) 1951 Collagen fibers of bony tissue in the electron microscope Huber andRoullier (1951) 1952 Acid-mediated demineralization of dental tissues for electron microscopy Albright et al (1952); Scott (1952Scott ( ) 1952 EDTA-mediated demineralization of bone for electron microscopy Watson (1952) 1953 Isolation of collagen from mammalian bone using dilute HCl Eastoe andEastoe (1953) 1954 First report about the presence of amino acids in fossil bones and shells up to approximately 350 M years old…”

Structure of the Nacreous Organic Matrix of a Bivalve Mollusk Shell Examined in the Hydrated State Using Cryo-TEM

“…The pioneering studies of Grégoire and his colleagues (Grégoire et al, 1955) revealed the complexity of the mollusk shell organic matrix. They used the transmission electron microscope (TEM) to show that the matrix sheets have fibrillar reticulate structures with variable pore sizes and shapes (Grégoire, 1962;Iwata, 1975).…”

Biochemical Change at the Setting-up of the Crossed-Lamellar Layer in Nerita undata Shell (Mollusca, Gastropoda)

“…But these crystallized exoskeletons are also long known to be composite materials: they indeed incorporate many organic macromolecules, that are associated to the mineral phase down to a fine scale [4][5][6][7][8][9]. These organic compounds are secreted by a mineralizing tissue, a dedicated part of the mollusc mantle epithelium.…”