La surexpression de l'androgen-binding protein (ABP) provoque des modifications morphologiques et fonctionnelles dans le testicule de souris

Estéban, Cristina; Gérard, A.; Larriba, S.; Torán, Núria; Nadal, M.; Plaja, Alberto; Martinez, D.; Martinez, Orianne; Benedit, Patricia; Gérard, Hubert; Reventós, Jaume; Munell, Francina

doi:10.1007/bf03034549

Cited by 2 publications

(1 citation statement)

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“…Further, homozygous mice presented an increased accumulation of pachytene and metaphase spermatocytes with abnormal morphology and localization, and these cells underwent a significant level of apoptosis ( Figure 4) (Larriba et al, 1995;Esteban et al, 1997a;Joseph et al, 1997a;Selva et al, 2000). By immunohistochemistry, accumulation of rat SHBG/ABP protein was detected in pachytene spermatocytes and metaphase cells in those tubules presenting arrest of spermatogenesis (Esteban et al, 1997b). Given that the level of SHBG/ABP is low during meiosis in rats (Ritzén et al, 1982), we speculated that an excess of SHBG/ABP in these stages of spermatogenesis led to the meiotic arrest and cellular degeneration detected in the transgenic mice and suggested that excess SHBG/ABP could have a negative control on spermatogenic progression at the level of the first meiotic division of primary spermatocytes (Selva et al, 2000).…”

Section: Role Of Shbg/abp In Reproductionmentioning

confidence: 97%

Androgen‐Binding Protein and Reproduction: Where Do We Stand?

MUNELL,

SUÁREZ‐QUIAN,

SELVA

et al. 2002

Journal of Andrology

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Section: Role Of Shbg/abp In Reproductionmentioning

confidence: 97%

Androgen‐Binding Protein and Reproduction: Where Do We Stand?

MUNELL,

SUÁREZ‐QUIAN,

SELVA

et al. 2002

Journal of Andrology

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Meiotic Arrest and Germ Cell Apoptosis in Androgen-Binding Protein Transgenic Mice*

et al. 2000

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The fundamental role of androgen-binding protein (ABP) in spermatogenesis remains obscure after nearly 25 yr since its first characterization. In the present investigation, we used a transgenic mouse model that overexpresses rat ABP to examine the potential involvement of this protein in the regulation of processes occurring during spermatogenesis. Specifically, homozygous or heterozygous transgenic mice were analyzed in terms of spermatogenic progression, DNA fragmentation pattern, and germinal cell ploidy status. All animals homozygous for transgenic ABP exhibited an increased accumulation of primary spermatocytes and cells at metaphase with abnormal morphology and localization within the seminiferous epithelium. Analysis of DNA fragmentation by in situ techniques and agarose gel electrophoresis provided evidence for an increased occurrence of apoptosis in the transgenic animals, principally involving pachytene spermatocytes and cells at metaphase. Flow cytometric analysis of the DNA content of isolated germ cells revealed a reduction in the number of haploid cells, an increase in the number of tetraploid cells, and the appearance of a hypotetraploid cell population, consistent with degenerating primary spermatocytes. In mice heterozygous for the transgene, the effects were less prominent, and the degree to which spermatogenesis was compromised correlated with the levels of ABP messenger RNA in individual animals. The present results are interpreted to suggest that ABP can act as a modulator of spermatogenesis by regulating completion of the first meiotic division of primary spermatocytes.

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La surexpression de l'androgen-binding protein (ABP) provoque des modifications morphologiques et fonctionnelles dans le testicule de souris

Cited by 2 publications

References 22 publications

Androgen‐Binding Protein and Reproduction: Where Do We Stand?

Androgen‐Binding Protein and Reproduction: Where Do We Stand?

Meiotic Arrest and Germ Cell Apoptosis in Androgen-Binding Protein Transgenic Mice*

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