La réaction d'hémagglutination passive pour le sérodiagnostic de la syphilis (T.P.H.A.)

Automation of a flocculation test for syphilis on Groupamatic equipment.

“…Finally, the tested samples were identical with the two automatic techniques. We can but state these results and bring to mind what some of us have found recently (Paris-Hamelin, Vaisman, and Fustec-Ibarboure, 1974) regarding the basic antigen used in the RPR card test and the ART (automatic reagin test); this antigen used manually on plasma, or on serum gave excellent results; the use of a continuous-flow autoanalyzer was so disappointing that it had to be given up. The ART antigen was not at fault, but rather the modified technique for this type of automatic equipment.…”

“…The earliest appear 30 to 40 days after infection, about 2 weeks after the appearance of the primary lesion. Antibodies detected by immunofluorescence reactions are the earliest closely followed by those detected by passive haemagglutination (TPHA) (Vaisman, Paris-Hamelin, and Fustec-Ibarboure 1974), then reagins and anti-Reiter protein (group) antibodies appear simultaneously. Antibodies detected by the TPI test are the last to appear.…”