La protein regulates protein expression by binding with the mRNAs of target genes and participates the pathological process of ovarian cancer

Abstract

Research on the mechanism and new targets of ovarian cancer is of great significance to reduce the high mortality and drug resistance of ovarian cancer. Human La protein has been found to be highly expressed in a variety of malignant tumors and plays a role in tumorigenesis and development through its RNA-binding function. However, its role and mechanism in ovarian cancer are not completely clear. The present study showed that La protein was highly expressed in serum and tissues of patients with ovarian cancer by ELISA and immunohistochemistry, and the high expression of La protein was associated with the increased degree of malignancy and poor prognosis by searching the KM plotter database. Interference of the La gene resulted in a significant decrease in the proliferation, migration, and invasion of ovarian cancer cells with growth block in the G1 phase and increasing apoptosis. By RNA binding protein immunoprecipitation, transcriptome sequencing, and proteomics, 14 downstream target genes were screened. The La protein might affect the protein expression of these 14 genes by binding with the mRNAs. Therefore, it played a role in the pathological process of ovarian cancer.

Compounds from Morchella esculenta as Potential Inhibitors of RNA-Binding Protein La in Ovarian Cancer: A Molecular Modeling and Quantum Mechanics Approach

“…According to Huang et al [30], OC patients have significantly higher levels of La protein expression in their serum than healthy individuals, which is related to the poor response to platinum-based chemotherapy. Furthermore, when the expression of La interfered with La RNAi in A2780 and SKOV3 cells, its impaired cell growth, which suggests that inhibiting the overexpression of La protein could promote apoptosis and inhibits the invasion and migration of OC cells.…”

“…Furthermore, when the expression of La interfered with La RNAi in A2780 and SKOV3 cells, its impaired cell growth, which suggests that inhibiting the overexpression of La protein could promote apoptosis and inhibits the invasion and migration of OC cells. Notably, Huang et al [30] reported the potential downstream target genes of La protein involved in the development and drug resistance of OC, including CALR, NES, PKM, RIF1, HLA-B, IGFRBP1, NQQ1, TK1, FHIT, MAP2K1, and SLC2A1. Inhibiting La protein could control the expression of these genes in impeding proliferation, invasion, migration, and chemotherapy resistance to OC [30].…”

“…Notably, Huang et al [30] reported the potential downstream target genes of La protein involved in the development and drug resistance of OC, including CALR, NES, PKM, RIF1, HLA-B, IGFRBP1, NQQ1, TK1, FHIT, MAP2K1, and SLC2A1. Inhibiting La protein could control the expression of these genes in impeding proliferation, invasion, migration, and chemotherapy resistance to OC [30].…”