La niche des cellules souches tumorales dans le glioblastome : des aspects fondamentaux au ciblage thérapeutique

Turpin, Anthony; Sharif, Ariane; Stoven, Luc; Blond, S.; Maurage, Claude‐Alain; Rhun, Émilie Le

doi:10.1016/j.bulcan.2014.07.001

Cited by 7 publications

(3 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hypoxia directly damages endothelial cells by destroying the cytoskeleton and intercellular connections, as well as disrupting metabolic and synthetic function, increasing the permeability of endothelial cells. The hypoxic niche of vascular endothelial cells promoted stemness maintenance and tumor propagation in cancer stem cells ( Turpin et al, 2015 ). HIF-1 alpha (Hypoxia inducible factor 1 subunit alpha) mediated responses to hypoxia in central nervous system neurovascular niches inducing several signaling molecules, including BDNF (Brain derived neurotrophic factor), VEGF, and stromal cell-derived factor 1, which were involved in orchestrated angiogenesis and neurogenesis ( Madri, 2009 ).…”

Section: Part Three: Changes In Vascular Endothelial Nichesmentioning

confidence: 99%

The Role and Mechanism of the Vascular Endothelial Niche in Diseases: A Review

Lei

et al. 2022

Front. Physiol.

View full text Add to dashboard Cite

Vascular endothelial cells, forming the inner wall of the blood vessels, participate in the body’s pathological and physiological processes of immunity, tumors, and infection. In response to an external stimulus or internal pathological changes, vascular endothelial cells can reshape their microenvironment, forming a “niche”. Current research on the vascular endothelial niche is a rapidly growing field in vascular biology. Endothelial niches not only respond to stimulation by external information but are also decisive factors that act on neighboring tissues and circulating cells. Intervention through the vascular niche is meaningful for improving the treatment of several diseases. This review aimed to summarize reported diseases affected by endothelial niches and signal molecular alterations or release within endothelial niches. We look forward to contributing knowledge to increase the understanding the signaling and mechanisms of the vascular endothelial niche in multiple diseases.

show abstract

Section: Part Three: Changes In Vascular Endothelial Nichesmentioning

confidence: 99%

The Role and Mechanism of the Vascular Endothelial Niche in Diseases: A Review

Lei

et al. 2022

Front. Physiol.

View full text Add to dashboard Cite

show abstract

“…Glioblastoma (GBM) classi ed as grade IV gliomas are one of the most aggressive and lethal tumors of the Central Nervous System (CNS) with median survival of less than 15 months [1][2][3][4]. Cancer stem cell hypothesis in GBM suggest that these tumors are driven by a small subpopulation of cells or glioma stem cell (GSCs) which are endowed with self-renewal properties and elevated DNA damage response pathways to prevent therapeutic insult [2,[5][6][7][8][9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Human Glioma Endothelial Cells With Stem cell properties

Sharma¹,

Muzumdar

Shiras

2022

Preprint

View full text Add to dashboard Cite

Glioblastoma (GBM) tumor relapse is attributed to presence of therapy defying Glioma stem cells (GSCs). GSCs have been shown to trans-differentiate into endothelial-like cells. However, the influence of tumor micro-environment on endothelial cells is not clearly understood. Here, we investigated whether tumor micro-environment conditioning can alter endothelial cell phenotype and endow them with stem cell-like properties. For this, we establishing a battery of primary human glioma endothelial cell cultures (hGECs) and characterized them for purity in multiple in vitro, in vivo assays. Our data shows that hGECs harbored stemness and multi-lineage differentiation potential as assessed in serum free growth assay, sphere forming assay, limiting dilution assay, and in a serum-induced differentiation assay where Nestin and CD31 co-expressing hGECs could spontaneously differentiation into GFAP positive cells. Moreover, immunohistochemistry analysis of human GBM tumors showed that tumor vessel regions expressed two key stem cell specific markers Nestin and Mushashi. Together, our data shows that tumor-specific endothelial cells are enriched with stem cell properties in GBM.

show abstract

“…Glioma stem cells (GSCs) are tumor cells possessing the characteristics and heterogeneity of stem cells [3], which are closely related to the occurrence, progression and outcome of brain tumors [6,7]. Research has found that GSCs maintain the growth of brain tumors in the long term and play an important role in tumor recurrence following conventional therapies.…”

mentioning

confidence: 99%

OCT4 is up-regulated by DNA hypomethylation of promoter in recurrent gliomas

Sun¹,

Shi²,

Ni³

et al. 2016

neo

View full text Add to dashboard Cite

OCT4, a marker of embryonic stem cells, is also a key transcription factor that plays a regulatory role in the self-renewal, proliferation and differentiation of stem cells. Previous studies showed that DNA methylation is involved in the regulation of OCT4 expression during the development and differentiation of embryonic stem cells. However, DNA methylation in the promoter region of OCT4 has not yet been discussed in human recurrent glioma. In this study, we assessed the specimens from 24 cases of recurrent glioma for OCT4 expression and methylation status, and commenced analyzing the correlation between the two by treating glioma cells with a demethylating agent in vitro. The results demonstrated that for the same cases, the expression of OCT4 in specimens of recurrent glioma was significant higher than that in primary glioma (P<0.05). DNA methylation levels in recurrent glioma decreased obviously compared with that in primary glioma (t=9.800, P=0.008). In vitro study indicated, following demethylation treatment, glioma cells had an increased OCT4 expression. These results suggest that DNA hypomethylation may be a key mechanism underlying the up-regulation of OCT4 in the recurrence of glioma, which facilitates the understanding of the role of stem cells and the exploration of novel strategies for the treatment of recurrent glioma.

show abstract

La niche des cellules souches tumorales dans le glioblastome : des aspects fondamentaux au ciblage thérapeutique

Cited by 7 publications

References 61 publications

The Role and Mechanism of the Vascular Endothelial Niche in Diseases: A Review

The Role and Mechanism of the Vascular Endothelial Niche in Diseases: A Review

Human Glioma Endothelial Cells With Stem cell properties

OCT4 is up-regulated by DNA hypomethylation of promoter in recurrent gliomas

Contact Info

Product

Resources

About