La metilación global del ADN y los niveles de homocisteína en plasma se encuentran disminuidos en pacientes con diabetes mellitus tipo 1

Arroyo-Jousse, Viviana; García-Díaz, Diego F.; Pérez-Bravo, Francisco

doi:10.4067/s0034-98872015000500002

Cited by 7 publications

(3 citation statements)

References 39 publications

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“…On the other hand, some studies refuted the link between HHcy and incidence of retinopathy in diabetic patients [40,41]. Indeed, it was reported that T1DM patients had lower levels of Hcy and global DNA methylation [42]. A recent study reported that elevated Hcy levels were associated with an increased risk of DR, especially in T2DM patients [43].…”

Section: Discussionmentioning

confidence: 99%

Homocysteine: A Potential Biomarker for Diabetic Retinopathy

Tawfik

Mohamed

Elsherbiny

et al. 2019

JCM

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Diabetic retinopathy (DR) is the most common cause of blindness in people under the age of 65. Unfortunately, the current screening process for DR restricts the population that can be evaluated and the disease goes undetected until irreversible damage occurs. Herein, we aimed to evaluate homocysteine (Hcy) as a biomarker for DR screening. Hcy levels were measured by enzyme-linked immuno sorbent assay (ELISA) and immunolocalization methods in the serum, vitreous and retina of diabetic patients as well as in serum and retina of different animal models of DM representing type 1 diabetes (streptozotocin (STZ) mice, Akita mice and STZ rats) and db/db mice which exhibit features of human type 2 diabetes. Our results revealed increased Hcy levels in the serum, vitreous and retina of diabetic patients and experimental animal models of diabetes. Moreover, optical coherence tomography (OCT) and fluorescein angiography (FA) were used to evaluate the retinal changes in mice eyes after Hcy-intravitreal injection into normal wild-type (WT) and diabetic (STZ) mice. Hcy induced changes in mice retina which were aggravated under diabetic conditions. In conclusion, our data reported Hcy as a strong candidate for use as a biomarker in DR screening. Targeting the clearance of Hcy could also be a future therapeutic target for DR.

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Section: Discussionmentioning

confidence: 99%

Homocysteine: A Potential Biomarker for Diabetic Retinopathy

Tawfik

Mohamed

Elsherbiny

et al. 2019

JCM

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“…Commonly, increased plasma homocysteine is considered as an independent risk factor for coronary artery disease, peripheral vascular disorder and thrombosis [ 45 ]. However, reduced circulating homocysteine have been reported in some populations with T1DM (from children to adults) compared with control [ 46 – 48 ] and plasma homocysteine showed a positive correlation with age in diabetic patients [ 47 ]. Therefore, in some populations with young age, the low concentration of homocysteine (under the threshold of 15µM) may not statistically represent the risk of cardiovascular events but acts as diagnostic biomarkers for distinguish MODY2 and T1DM, though the possible mechanism remains to be explored.…”

Section: Discussionmentioning

confidence: 99%

Quantitative profiling and diagnostic potential of one-carbon and central metabolism pools in MODY2 and T1DM

Liu,

Xie,

et al. 2023

Diabetol Metab Syndr

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Background Maturity-onset diabetes of the young type 2 (MODY2) is a rare genetic disorder characterized as mild fasting hyperglycemia with low risk of vascular complications caused by glucokinase gene mutation. This study aims to investigate metabolites alteration associated with MODY2, exploring possible mechanism underlying characteristic clinical manifestations and low cardiovascular risks of MODY2 and providing serum metabolite biomarkers to facilitating MODY2 diagnosis. Methods Fasting serum samples from MODY2, type 1 diabetes (T1DM) and healthy individuals were collected. By using targeted metabolomics via liquid chromatography–tandem mass spectrometry platform, we quantified the metabolites involved in tricarboxylic acid (TCA) cycle and one-carbon metabolism. Results Metabolomic profiling revealed significant difference of intermediates from central metabolism cycle, methionine cycle and several amino acids between MODY2 and T1DM groups. Among these, serum citrate, α-ketoglutaric acid, serine, glycine, glutamine and homocysteine were significantly elevated in MODY2 patients compared with T1DM patients; and compared with healthy subjects, malate and methionine levels were significantly increased in the two groups of diabetic patients. The correlation analysis with clinical indexes showed that α- ketoglutarate, serine, glycine, and glutamine were negatively correlated with blood glucose indicators including fasting blood glucose, HbA1c, and GA, while citrate was positively correlated with C-peptide. And homocysteine displayed positive correlation with HDL and negative with C-reactive protein, which shed light on the mechanism of mild symptoms and low risk of cardiovascular complications in MODY2 patients. A panel of 4 metabolites differentiated MODY2 from T1DM with AUC of 0.924, and a combination of clinical indices and metabolite also gained good diagnostic value with AUC 0.948. Conclusion In this research, we characterized the metabolite profiles of TCA cycle and one-carbon metabolism in MODY2 and T1DM and identified promising diagnostic biomarkers for MODY2. This study may provide novel insights into the pathogenesis and clinical manifestations of MODY2.

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“…La metilación se lleva a cabo por acción de las DNA metiltransferasas (DNMT), las cuales añaden grupos metilo en la posición 5' de la citosina del dinucleótido CpG, el cual se encuentra repetido en estructuras llamadas islas CpG, en los genomas de los mamíferos, éstas llegan a tener una extensión de 300 a 3,000 pares de bases, las cuales se encuentran cercanas a las regiones promotoras, donde se lleva a cabo el inicio de la transcripción, lo que permite la regulación genética por esté mecanismo 36,37 . La adición de grupos metilo al DNA no permite la separación de ambas hebras, evitando la unión de la maquinaria de transcripción y la formación de nuevas moléculas de RNA.…”

Section: Metilación Del Dnaunclassified

La Nutrición como modulador epigenético y su papel en la prevención de enfermedades

Vasquez

Ramírez‐Moreno

Zafra-Rojas

et al. 2021

ICSA

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La malnutrición, ya sea por la falta o por el exceso de algún componente de la dieta, produce un estado de estrés oxidativo, que dañan a las diferentes biomoléculas, además de producir modificaciones epigenéticas heredables, mediante cambios en la estructura de DNA pero sin alterar la secuencia de los nucleótidos, lo que resultan en el desarrollo de enfermedades que son trasmitidas a las siguientes generaciones. El objetivo del presnte artículo fue hacer una revisión en la literatura sobre el daño oxidativo y las modificaciones epigenéticas y el papel de la nutriepigenómica en la prevención y tratamiento de enfermedades. Se realizó una revisión bibliográfica en PubMed, Cochrane y Google Académico, sobre estrés oxidativo, modificaciones epigenéticas y nutriepigenómica, para determinar su efecto en el en el binomio salud-enfermedad. Los reultados muestran que la malnutrición, da origen de diversas enfermedades, a través del daño oxidativo y modificaciones epigenéticas lo cual afecta a los individuos y su descendencia. Por lo que la nutriepigenómica, puede reprogramar el epigenóma del individuo mediante intervenciones nutricionales. Nosotros cuncluimos que la nutrición juega un papel importante en la prevención del daño oxidativo y modulación del epigenóma, con lo cual se puede lograr mantener un buen estado de salud de la población y de las futuras generaciones.

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La metilación global del ADN y los niveles de homocisteína en plasma se encuentran disminuidos en pacientes con diabetes mellitus tipo 1

Abstract: (r = -0.564, p < 0.01) and healthy subjects (r = 0.440, p = 0.03 (Rev Med Chile 2015; 143: 562-568)

Cited by 7 publications

References 39 publications

Homocysteine: A Potential Biomarker for Diabetic Retinopathy

Homocysteine: A Potential Biomarker for Diabetic Retinopathy

Quantitative profiling and diagnostic potential of one-carbon and central metabolism pools in MODY2 and T1DM

La Nutrición como modulador epigenético y su papel en la prevención de enfermedades

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