La coloración de Ziehl-Neelsen en histopatología

Naranjo, Patricia Jaramillo de; Rodríguez, Gerzaín; Rodríguez, Angeles; Caldas, María Leonor

doi:10.7705/biomedica.v8i3-4.1964

Cited by 6 publications

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“…A drop was deposited on a slide for each sample, making the fixation of 1 × 0.5 cm 2 approximate. The slide was left to dry at room temperature to perform the modified Ziehl‐Neelsen staining technique 19,20 . The sample was considered positive, once the presence of spherical elements of 5 μm in diameter, fuchsia colour (acid‐alcohol resistant) and irregularly stained concordant with oocysts Cryptosporidium spp 21 …”

Section: Methodsmentioning

confidence: 99%

Prevalence of Cryptosporidium spp. in Horses from communities of the Mapuche native people, Araucanía Region, Chile

Tuemmers

Fellenberg

Pérez

et al. 2022

Equine Veterinary Journal

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Background:There are no studies of potential zoonotic diseases in Mapuche communities' horses. Objectives:The objective of this study was to determine the prevalence of Cryptosporidium in horses of the Mapuche communities. Study design:This was a cross-sectional study.Methods: Faecal samples from 100 randomly selected horses (n = 100) were taken from rural Mapuche communities from four municipalities from the Araucanía Region.These samples were processed with the modified Ziehl-Neelsen staining technique and grouped by sex, age and municipality. Results:The general prevalence of Cryptosporidium spp. was 67.0% (n = 67). The prevalence was 51.0% (n = 51) in males and 49.0% (n = 49) in females, and there is no gender association to the presentation of Cryptosporidium spp. The prevalence by municipality was 60.0%, 80.0%, 64.0% and 64.0% in Curarrehue, Lonquimay, Padre las Casas and Teodoro Schmidt, respectively. The above shows no significant association between the sector and the presence of Cryptosporidium spp. The prevalence by age was 95.4% of horses tested positive for Cryptosporidium between birth and 6 years of age. About 27.3% of horses were tested positive in the age group between 7 and 10 years. There was no presence of Cryptosporidium spp. in the age group older than 11 years, showing a significant relationship between the age of the animal and the presence of Cryptosporidium (P ˂ 0.05). Main limitations:The sample analysis did not specifically identify the type of Cryptosporidium, and it was not possible to evaluate the zoonotic risk in the Mapuche communities.Conclusions: Cryptosporidium spp. is present in working horses in Mapuche communities, with a 67.7% general prevalence, and there is a significant association between this parasite and the age of the horses, being higher in the age group between 0 and 6 years, with a prevalence of 95.4%. There may be a potential zoonotic risk in the Mapuche communities.

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Section: Methodsmentioning

confidence: 99%

Prevalence of Cryptosporidium spp. in Horses from communities of the Mapuche native people, Araucanía Region, Chile

Tuemmers

Fellenberg

Pérez

et al. 2022

Equine Veterinary Journal

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“…We The biopsies from the lesions were processed with hematoxylin and eosin (HE) and Ziehl Neelsen stain [31].…”

Section: Introductionmentioning

confidence: 99%

Iatrogenic outbreak of M. chelonae skin abscesses

et al. 1996

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SUMMARYWe describe an outbreak of skin lesions due to Mycobacterium chelonae subsp. abscessus associated with injections of lidocaine (lignocaine) given by a 'bioenergetic' (a practitioner of alternative medicine) in Colombia. The lidocaine carpules and the lesions of the patients yielded mycobacteria with identical biochemical characteristics.Using the methodology of Sartwell and a case control design we examined the incubation period and assessed risk factors. Of 667 potentially exposed individuals, a total of 298 patients were interviewed, of whom 232 had skin lesions. The median incubation period was 305 days (range 15-59 days). Male sex (OR 2-85, 95 % CI 126-6-5 1), increasing age (OR 1-25, 95 % CI 103-1-53), subcutaneous injection route (OR 3-72, 95 % CI 1-09-12-7) and number of injections (OR 101, 95% CI 1001-03) were risk factors for disease.To our knowledge, this is the largest reported outbreak of M. chelonae infection, the first in which the organism has been isolated from the putative vehicle of infection, and the first in which the incubation period could be determined.

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“…Los patólogos deben tener en cuenta este hecho porque es común solicitar la coloración de plata metenamina, PAS y ZN en el estudio de procesos inflamatorios supurativos y granulomatosos del ganglio linfático. Es importante resaltar que el bacilo de Hansen es menos ácido-alcohol resistente que el bacilo de Koch y que las técnicas para teñirlos no son equivalentes (1,22). Para demostrar el bacilo de Hansen se hace una técnica que demuestra su ácido-resistencia al H 2 SO 4 al 5%; es esencial hacer la coloración con el control adecuado, que debe ser un caso de lepra y no uno de tuberculosis (1,22).…”

Section: Patologíaunclassified

Adenopatías generalizadas como presentación de la reacción leprótica tipo 2.

Rodríguez¹

2003

biomedica

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Las reacciones en los pacientes con lepra son manifestaciones clínicas graves de inflamación aguda en las lesiones crónicas del enfermo, capaces de producir daño irreversible e incapacitante. Estudiamos un hombre de 46 años con reacción leprótica tipo 2, que consultó por fiebre, malestar general, sensación de obstrucción nasal, nódulos cutáneos y adenopatías generalizadas. El cuadro hemático mostró leucocitosis con neutrofilia. Entre varios diagnósticos clínicos sugeridos no se pensó en lepra. Una biopsia ganglionar demostró necrosis extensa del órgano que estaba infiltrado por polimorfonucleares y macrófagos espumosos, con necrosis de vénulas y depleción linfoide. No se hizo coloración de ZN, pero sí de Gomori, que tiñó muy bien los bacilos de Hansen, pero no se detectaron por el patólogo, que no hizo un diagnóstico concluyente. Veinte meses después, el paciente presentó síntomas semejantes con adenopatías generalizadas y nódulos cutáneos más numerosos, la biopsia de uno de los cuales demostró lepra lepromatosa con eritema nodoso leproso o reacción tipo 2. El tratamiento antileproso con poliquimioterapia y antirreaccional con talidomida curó al paciente, que 3 años después no presenta secuelas, pese a los 20 meses transcurridos para precisarse el diagnóstico. Comentamos este caso clínico y revisamos los factores predisponentes, la histopatología, los diagnósticos diferenciales de la adenopatía leprosa, la patogenia, el pronóstico y el tratamiento de la reacción tipo 2 en lepra, que constituye una urgencia médica, capaz de originar incapacidad grave y que, como en este enfermo, puede cursar con adenopatías como signos y síntomas predominantes.Palabras clave: reacción leprótica, adenopatía leprosa, eritema nodoso leproso, reacción tipo 2. Generalized adenopathy as a manifestation of type 2 reactional leprosyLeprosy patient's reactions are severe clinical manifestations of acute inflammation of chronic lesions, capable of producing irreversible and invalidating damage. We studied a 46 year-old man with a type 2 leprosy reaction, who presented fever, cutaneous nodules, nasal obstruction and generalized adenopathy. The hemogram showed leucocytosis with neutrophilia. None of the initial diagnoses included leprosy. A lymph node biopsy revealed extensive necrotic areas infiltrated with polymorphonuclear lymphocytes, and foamy macrophages. Eosinophylic necrosis and thrombosis of venules with lymphoid nodule depletion was also in evidence. Ziehl Neelsen stain was not done, but the Gomori stain clearly showed Hansen's bacilli. These were were not detected by the pathologist and therefore a final diagnosis was not provided. Twenty months later, the patient presented similar symptoms, but with more generalized lymphadenopathy and presence of cutaneous nodules. Nodule biopsy showed lepromatous leprosy with erythema nodusum leprosum or type 2 reaction. Polychemotherapy treatment and anti-reaction treatment with thalidomide cured the patient. No sequelae were noted in 3 years following the treatment. A literature review of the...

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La coloración de Ziehl-Neelsen en histopatología

Cited by 6 publications

References 0 publications

Prevalence of Cryptosporidium spp. in Horses from communities of the Mapuche native people, Araucanía Region, Chile

Prevalence of Cryptosporidium spp. in Horses from communities of the Mapuche native people, Araucanía Region, Chile

Iatrogenic outbreak of M. chelonae skin abscesses

Adenopatías generalizadas como presentación de la reacción leprótica tipo 2.

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