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Bella, Deborah; Hirschberger, Lawrence L.; Kwon, Young Hye; Stipanuk, Martha H.

doi:10.1007/s00726-002-0213-z

Cited by 23 publications

(5 citation statements)

References 29 publications

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“…Quantitative real time PCR with 10 ng of cDNA were carried out in duplicate in a 7300 real time PCR system (Effendorff, Hauppauge, NY) using the SYBR master mix (Qiagen) and the following cycles: 2 min at 50°C, 10 min at 95°C, and then 40 cycles each at 95°C for 15 s and 60°C for 60 s [19]. RT 2 qPCR analysis was also carried out according to manufacturer's manual using β -actin as a control.…”

Section: Methodsmentioning

confidence: 99%

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Development of a Novel Cysteine Sulfinic Acid Decarboxylase Knockout Mouse: Dietary Taurine Reduces Neonatal Mortality

Park

Dobkin

et al. 2014

Journal of Amino Acids

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We engineered a CSAD KO mouse to investigate the physiological roles of taurine. The disruption of the CSAD gene was verified by Southern, Northern, and Western blotting. HPLC indicated an 83% decrease of taurine concentration in the plasma of CSAD−/−. Although CSAD−/− generation (G)1 and G2 survived, offspring from G2 CSAD−/− had low brain and liver taurine concentrations and most died within 24 hrs of birth. Taurine concentrations in G3 CSAD−/− born from G2 CSAD−/− treated with taurine in the drinking water were restored and survival rates of G3 CSAD−/− increased from 15% to 92%. The mRNA expression of CDO, ADO, and TauT was not different in CSAD−/− compared to WT and CSAD mRNA was not expressed in CSAD−/−. Expression of Gpx 1 and 3 was increased significantly in CSAD−/− and restored to normal levels with taurine supplementation. Lactoferrin and the prolactin receptor were significantly decreased in CSAD−/−. The prolactin receptor was restored with taurine supplementation. These data indicated that CSAD KO is a good model for studying the effects of taurine deficiency and its treatment with taurine supplementation.

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Section: Methodsmentioning

confidence: 99%

“…Cysteamine is oxidized to hypotaurine by ADO. Hepatic CSAD and CDO activity is high compared to that in the kidney and brain [15, 19–21]. The tissue distribution of CSAD, protein, and mRNA, detected by Western and Northern blot analyses, is consistent with CSAD activity.…”

Section: Introductionmentioning

confidence: 96%

Development of a Novel Cysteine Sulfinic Acid Decarboxylase Knockout Mouse: Dietary Taurine Reduces Neonatal Mortality

Park

Dobkin

et al. 2014

Journal of Amino Acids

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“…High protein concentrations in the diet significantly enhance cysteine desulfhydration [6]. Cysteine dioxygenase activity is increased in response to increasing levels of dietary methionine at post-transnlational levels due to suppression of degradation of the enzyme via polyubiquitination [7,8]. Methionine synthase levels are increased by homocysteine at the transcriptional level [9].…”

Section: The Mercaptopyruvate Pathway and Mercaptopyruvate Sulfurtranmentioning

confidence: 99%

The Mercaptopyruvate Pathway in Cysteine Catabolism: A Physiologic Role and Related Disease of the Multifunctional 3-Mercaptopyruvate Sulfurtransferase

Nagahara

Sawada²

2006

CMC

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The conversion of cysteine to 3-sulfino-alanine is a major pathway in cysteine catabolism. Cysteine dioxygenase catalyzes the reaction and dietary intake of the essential amino acid methionine and the semi-essential amino acid cysteine increases the level of this enzyme by suppressing enzyme degradation via polyubiquitination. The production of cellular antioxidants such as glutathione, thioredoxin, and their families is important in cysteine metabolism, and these cellular antioxidants have critical roles in the maintenance of the cellular redox status. The mercaptopyruvate pathway, in which cysteine or aspartate transaminase catalyzes the transamination from cysteine to 3-mercaptopyruvate and then 3-mercaptopyruvate sulfurtransferase catalyzes the transsulfuration from 3-mercaptopyruvate to pyruvate, also contributes to maintain the cellular redox. 3-Mercaptopyruvate sulfurtransferase serves as an antioxidant protein: when the enzyme is exposed to stoichiometric amounts of the oxidant hydrogen peroxide, it is inhibited via the formation of low redox sulfenate at the catalytic site cysteine. On the other hand, activity is restored by the reductant dithiothreitol or reduced thioredoxin. 3-Mercaptopyruvate sulfurtransferase also detoxifies cyanide via transsulfuration from a stable persulfide at the catalytic site cysteine, a reaction intermediate, suggesting that cyanide detoxification is not necessarily an enzymatic reaction. Furthermore, a congenital defect of the enzyme causes mercaptolactate-cysteine disulfiduria associated with or without mental retardation, although the pathogenesis remains unclear. These facts suggest that 3-mercaptopyruvate sulfurtransferase has physiologic roles as an antioxidant and a cyanide antidote; is essential for neural function, and participates in cysteine degradation.

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“…Для підтвердження діагнозу було проведено контрольний забій, патолого-анатомічний розтин та гістологічне дослідження печінки курей-несучок віком 166, 300 і 530 діб. Варто зазначити, що основні зміни були виявлені в печінці, однак дані літератури свідчать про наявність патологічних змін в нирках та серці (Danilov & Berkovich, 1975;Butler, 1976;Bella et al, 2002).…”

Section: результати та їх обговоренняunclassified

Morphological particulars of the liver of laying hens of cross “Lohmann Brown” under hepatosis

2020

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The article presents the results of histological examinations of the liver of laying hens at age 166, 300 and 530 days, in which according to the results of biochemical analysis of blood serum revealed a subclinical course of hepatosis. The purpose of our study was the early diagnosis of this pathology and the study of the histological structure of the liver in different periods of poultry productivity. The work was performed at LLC Agro Firm “Zagai” of Kamianka-Buzkyi district of Lviv region. Autopsy and examination of histological specimens were performed at the Department of Normal and Pathological Morphology and Forensic Veterinary at Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv. The macroscopic examination of the liver showed that in part of laying hens at 166 days of age, in most of laying hens at 300 days old and in all of laying hens at 530 days old examined, the liver was slightly enlarged, of a sluggish consistency and unevenly colored. It should also be noted that, in addition to severe dystrophic changes in the liver, 530 days of laying hens also showed signs of general obesity. The study of histological specimens installed that in part of laying hens at 166 days of age, the liver lobules were disordered, in resulting was disrupted their beam structure. At the same time, cytoplasm of hepatocytes is somewhat cloudy and visible granularity. In laying hens at age 300 and 530 days installed the discomplexation of the beam structure of the organ and the presence of small and large droplets of fat vacuoles in hepatocytes are indicated, which indicate about the development of fatty dystrophy. The results of the morphological study indicate the presence in the liver of laying hens signs of acute and chronic pathological processes, which with age are complicated by dystrophic changes of the organ.

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Cited by 23 publications

References 29 publications

Development of a Novel Cysteine Sulfinic Acid Decarboxylase Knockout Mouse: Dietary Taurine Reduces Neonatal Mortality

Development of a Novel Cysteine Sulfinic Acid Decarboxylase Knockout Mouse: Dietary Taurine Reduces Neonatal Mortality

The Mercaptopyruvate Pathway in Cysteine Catabolism: A Physiologic Role and Related Disease of the Multifunctional 3-Mercaptopyruvate Sulfurtransferase

Morphological particulars of the liver of laying hens of cross “Lohmann Brown” under hepatosis

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