L929 fibroblast and Saos‐2 osteoblast response to hydroxyapatite‐βTCP/agarose biomaterial

Abstract

Biphasic calcium phosphate, a mixture of hydroxyapatite (HA) and beta-tricalcium phosphate (beta-TCP), has been successfully used as an excellent bone graft substitute because of the HA capacity for direct interaction with bone and the beta-TCP resorption properties. Agarose has been recently mixtured with ceramics as natural biodegradable binder to increase the biomaterial flexibility facilitating its placement into the bone defect. In this study, the behavior of L929 fibroblasts and Saos-2 osteoblasts cultured on hydroxyapatite-betaTCP/agarose disks has been evaluated. Both cell types adhere and proliferate on the biomaterial surface maintaining their characteristic morphology. Transitory changes on cell cycle, size, and complexity are observed. The biomaterial induces apoptosis in Saos-2 osteoblasts but not in fibroblasts. A transitory stimulation of fibroblast mitochondrial activity is observed. This effect remains in osteoblasts after 9 days of culture showing a higher sensitivity of this cell type. However, the intracellular reactive oxygen species content and the lactate dehydrogenase release of Saos-2 osteoblasts indicate that hydroxyapatite-betaTCP/agarose does not induce oxidative stress in this cell type and confirm the integrity of the osteoblast plasma membrane. These results underline the good biocompatibility of hydroxyapatite-betaTCP/agarose disks and its potential utility for bone substitution and repair.

Biocomposites and hybrid biomaterials based on calcium orthophosphates

“…The biocompatibility of this BCP/agarose system was tested with mouse L929 fibroblasts and human SAOS-2 osteoblasts during different colonization times. 859 Fibrin sealants are non-cytotoxic, fully resorbable biological matrices that simulate the last stages of a natural coagulation cascade, forming a structured fibrin clot similar to a physiological clot. 860 Biocomposites of calcium orthophosphates with fibrin sealants might help develop the clinical applications of bone substitutes.…”

In vitro osteoclast-like and osteoblast cells’ response to electrospun calcium phosphate biphasic candidate scaffolds for bone tissue engineering

“…Both cell lines were cultured as described in sections 2.4 and 2.5. [35,36]. After 6 days cells were trypsinized and resuspended in 1 ml of culture medium.…”

Anti-sessile bacterial and cytocompatibility properties of CHX-loaded nanohydroxyapatite

“…Hydroxyapatite, an inorganic calcium phosphate material, has long been preferred as the material used in hard tissue repair over autografts and allografts, drug carrier and coatings due to the chemical similarities with bone and teeth mineral enamel [21][22][23]. However, hydroxyapatite has some disadvantages such as brittleness, low tensile strength and fracture toughness [24].…”