L'utilisation d'un copolymère du groupe des polyesters comme matériel d'inclusion en ultramicrotomie

Kellenberger, E.; Ryter, A; Schwab, W

doi:10.1007/bf02157363

Cited by 118 publications

(15 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After fixation, the specimens were dehydrated in an ascending series of alcohol concentrations, stained for 1/~ hour in a 5 per cent solution (in alcohol) of phosphotungstic acid (PTA) and embedded in Vestopal W (13). Since the length and width of the microvilli change as cells migrate from the crypts to the villi, care was taken during trimming of the blocks of tissue to locate only the areas immediately below the tips of the villi, where the dimensions of the microvilli have been found to be constant.…”

Section: Methodsmentioning

confidence: 99%

Electron Microscope Studies of the Structure of the Microvilli on Principal Epithelial Cells of Rat Jejunum After Treatment in Hypo- And Hypertonic Saline

Millington

Finean

1962

The Journal of Cell Biology

View full text Add to dashboard Cite

Immersion of the intestinal tissue, from rat jejunum, in hypertonic saline produced very rapid changes in all regions of the epithelial cells, but the apical region was apparently unaffected by hypotonic solutions for at least ½ hour. In both cases, blistering of the microvilli was taken as the first sign of degenerative changes which finally resulted in a breakdown to large vesicular particles. Consideration of both normal and modified tissue indicates that the core of the microvillus contains either paired strands or tubular structures. Lateral cross-fibres extended from the core to the microvillus membrane and may be an essential part of the supporting structure of the microvillus. Densitometer traces across the microvillus membrane at various stages of modification indicated that this membrane might include a 75 A unit membrane structure with additional components associated at either surface. Interruptions in the membrane were apparently expanded by the hypotonic solutions and these might possibly be distinguished from preparative artefacts.

show abstract

Section: Methodsmentioning

confidence: 99%

Electron Microscope Studies of the Structure of the Microvilli on Principal Epithelial Cells of Rat Jejunum After Treatment in Hypo- And Hypertonic Saline

Millington

Finean

1962

The Journal of Cell Biology

View full text Add to dashboard Cite

show abstract

“…These resins have the advantages of Vestopal-like resins-polymerization with ultraviolet light, high contrast and low intrinsic background structure, without the disadvantage of Vestopal-very low polarity compared to HM20. The highly crosslinked resins are quite stable in the electron beam and are not prone to the melting phenomenon of methacrylate-based resins described by Kellenberger et al (1956) and specimen shrinkage during polymerization. The non-crosslinked resins were less stable when subjected to similar treatments in the microscope.…”

mentioning

confidence: 99%

Specimen preparation for electron microscopy using low temperature embedding resins

Armbruster

Carlemalm

Chiovetti

et al. 1982

Journal of Microscopy

Self Cite

217

View full text Add to dashboard Cite

show abstract

“…In most cases, the specimens were embedded in polyester resin. * This embedding material, first used in Kellenbergei's la bora ton, 12 proved to be much superior to methacrylate which was used with some of the specimens. Thin sections were mounted on carbon-coated or bare grids for electronmicroscopic examination.…”

Section: Ixtimal Thickening Of Arteries Histologlcal and Electron-micmentioning

confidence: 99%

Intimal Thickening After Ligature of Arteries An Electron-Microscopic Study

Buck

1961

Circulation Research

147

View full text Add to dashboard Cite

Concentric thickening of the tunica intima was observed in a short length of common carotid artery which had been tied off between two ligatures, and also in the pieces immediately proximal and distal to the doubly ligated part. The thickening was not due to hyperplasia of endothelial cells; in fact, the endothelium in the doubly ligated part was necrotic a short time after ligature. This piece then became lined with macrophage-like cells, thought to be derived from monocytes of the blood contained in the lumen. These cells retained quantities of colloidal thorium dioxide which had been given intravenously just be fore applying the ligatures. On the other hand, the thickening of the intima seen in the doubly ligated segment, as well as in the adjoining lengths of artery, was principally due to the growth of a cell derived from the smooth muscle of the tunica media, for which the term "myo-intimal" cell is suggested.

show abstract

L'utilisation d'un copolymère du groupe des polyesters comme matériel d'inclusion en ultramicrotomie

Cited by 118 publications

References 1 publication

Electron Microscope Studies of the Structure of the Microvilli on Principal Epithelial Cells of Rat Jejunum After Treatment in Hypo- And Hypertonic Saline

Electron Microscope Studies of the Structure of the Microvilli on Principal Epithelial Cells of Rat Jejunum After Treatment in Hypo- And Hypertonic Saline

Specimen preparation for electron microscopy using low temperature embedding resins

Intimal Thickening After Ligature of Arteries An Electron-Microscopic Study

Contact Info

Product

Resources

About