L-type calcium channels play a crucial role in the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells

Li, Wen; Wang, Yu; Wang, Huan; Kong, Ling‐Min; Zhang, Liang; Chen, Xin; Ding, Yin

doi:10.1016/j.bbrc.2012.06.128

Cited by 85 publications

(65 citation statements)

References 31 publications

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“…Moreover, calcium ions have been shown to stimulate osteoclast-medicated release of growth factors that enhance bone tissue remodeling. 8,[11][12][13][14][15] In this study, to enhance the scaffold's capacity for regenerating bone tissue, scaffolds were fabricated from HA and TCP materials. Furthermore, the quantity of calcium ions released from the ceramic scaffold was adjusted by the composition of HA and TCP because the degradation rate of TCP is faster than that of HA.…”

Section: Figmentioning

confidence: 99%

“…Calcium ions activate the chemotaxis of osteoblasts and osteogenic differentiation of stem cells, and stimulate osteoclasts to release growth factors. 8,[11][12][13][14][15] Among the materials for bone tissue scaffolds, calcium phosphate ceramics, hydroxyapatite [HA: Ca 10 (PO 4 ) 6 (OH) 2 ], and tricalcium phosphate [TCP: Ca 3 (PO 4 ) 2 ], which are the inorganic components of native bone tissue, release calcium ions during degradation. Moreover, these materials also have biocompatibility and osteoconductivity; thus, they are excellent materials for bone tissue regeneration.…”

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confidence: 99%

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Improvement of Bone Regeneration Capability of Ceramic Scaffolds by Accelerated Release of Their Calcium Ions

Seol

Park

Jung

et al. 2014

Tissue Engineering Part A

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To regenerate the bone tissue, the fabrication of scaffolds for better tissue regeneration has attracted a great deal of attention. In fact, growth factors are already used in clinical practice and are being investigated for enhancing the capacity for bone tissue regeneration. However, despite their strong osteoinductive activity, these growth factors have several limitations: safety issues, high treatment costs, and the potential for ectopic bone formation. The aim of this study was therefore to develop ceramic scaffolds that could promote the capacity for bone regeneration without growth factors. Three-dimensional ceramic scaffolds were successfully fabricated from hydroxyapatite (HA) and tricalcium phosphate (TCP) using projection-based microstereolithography, which is an additive manufacturing technology. The effects of calcium ions released from ceramic scaffolds on osteogenic differentiation and bone regeneration were evaluated in vitro and in vivo. The osteogenesis-related gene expression and area of new bone formation in the HA/TCP scaffolds was higher than those in the HA scaffolds. Moreover, regenerated bone tissue in HA/TCP scaffolds were more matured than that in HA scaffolds. Through this study, we were able to enhance the bone regeneration capacity of scaffolds not by growth factors but by calcium ions released from the scaffolds. Ceramic scaffolds developed in this study might be useful for enhancing the capacity for regeneration in complex bone defects.

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Section: Figmentioning

confidence: 99%

mentioning

confidence: 99%

Improvement of Bone Regeneration Capability of Ceramic Scaffolds by Accelerated Release of Their Calcium Ions

Seol

Park

Jung

et al. 2014

Tissue Engineering Part A

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“…Despite the large number of studies demonstrating the potential role of CaP minerals and Ca 2+ and PO 3− 4 on osteogenic differentiation of osteoblasts and progenitor cells, the molecular mechanism through which these ions regulate osteogenic commitment of stem cells remains largely unknown. Recent studies have shown that influx of extracellular Ca 2+ through L-type calcium channels promotes osteogenic differentiation of osteoprogenitor cells (28). However, very little is known about the mechanism through which PO 3− 4 supports osteogenesis.…”

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confidence: 99%

Calcium phosphate-bearing matrices induce osteogenic differentiation of stem cells through adenosine signaling

Shih

Hwang²,

Phadke³

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

310

265

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Synthetic matrices emulating the physicochemical properties of tissue-specific ECMs are being developed at a rapid pace to regulate stem cell fate. Biomaterials containing calcium phosphate (CaP) moieties have been shown to support osteogenic differentiation of stem and progenitor cells and bone tissue formation. By using a mineralized synthetic matrix mimicking a CaP-rich bone microenvironment, we examine a molecular mechanism through which CaP minerals induce osteogenesis of human mesenchymal stem cells with an emphasis on phosphate metabolism. Our studies show that extracellular phosphate uptake through solute carrier family 20 (phosphate transporter), member 1 (SLC20a1) supports osteogenic differentiation of human mesenchymal stem cells via adenosine, an ATP metabolite, which acts as an autocrine/paracrine signaling molecule through A2b adenosine receptor. Perturbation of SLC20a1 abrogates osteogenic differentiation by decreasing intramitochondrial phosphate and ATP synthesis. Collectively, this study offers the demonstration of a previously unknown mechanism for the beneficial role of CaP biomaterials in bone repair and the role of phosphate ions in bone physiology and regeneration. These findings also begin to shed light on the role of ATP metabolism in bone homeostasis, which may be exploited to treat bone metabolic diseases.bone metabolism | mineralized matrix | biomimetic material | phosphate signaling

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“…Voltage sensitive Ca 2+ channels (VSCCs) serve as crucial mediators of membrane excitability (Miller, 1987) and many Ca 2+ -dependent functions such as growth of bone (Duriez et al, 1993), regulate proliferation (Loza et al, 1994), differentiation (Wen et al, 2012), enzyme activity (Reuter 1983) and gene expression (Murphy et al, 1991). It also has been demonstrated that osteoblasts express VSCCs (Duncan et al, 1998;McDonald, 2004).…”

Section: Introductionmentioning

confidence: 99%

Interleukin-1B and 6-Induced Calcium Channel Current Modulation in MC3T3-E1 Osteoblast Cell Line

Kobayashi¹,

Endoh²,

Uchida³

et al. 2015

IJB

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Interleukin-1β (IL-1β) and -6 (IL-6) are inflammatory cytokines that are involved in bone resorption under pathological conditions. The cytokines are also involved in bone remodeling under physiological conditions. Voltage sensitive Ca 2+ channels (VSCCs) serve as crucial mediators of membrane excitability and many Ca 2+ -dependent functions such as growth of bone, regulate proliferation, differentiation, enzyme activity and gene expression. The effects of IL-1β and -6 on VSCCs in osteoblast cell line (MC3T3-E1) were investigated using patch-clamp recording. Our results showed that application of 50 pM-50 nM IL-1β facilitated VSCCs current (I Ca ) carried by Ba 2+ (I Ba ). Application of 50 pM-5 nM IL-6 facilitated I Ba . In contrast, 50 nM IL-6 inhibited I Ba in MC3T3-E1 cells. Treatment with MAPK inhibitor, PD98059, attenuated the 5 nM IL-6-induced facilitation of I Ba . Treatment with STAT3 inhibitor, staffic, attenuated the 50 nM IL-6-induced inhibition of I Ba . Treatment with PD98059 also attenuated the 50 nM IL-6-induced inhibition of I Ba . These results suggest that 5 nM IL-6 facilitates VDCCs involving MAPK pathways. In addition, 50 nM IL-6 inhibits VDCCs involving STAT3 and MAPK pathways in MC3T3-E1 cells.

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L-type calcium channels play a crucial role in the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells

Cited by 85 publications

References 31 publications

Improvement of Bone Regeneration Capability of Ceramic Scaffolds by Accelerated Release of Their Calcium Ions

Improvement of Bone Regeneration Capability of Ceramic Scaffolds by Accelerated Release of Their Calcium Ions

Calcium phosphate-bearing matrices induce osteogenic differentiation of stem cells through adenosine signaling

Interleukin-1B and 6-Induced Calcium Channel Current Modulation in MC3T3-E1 Osteoblast Cell Line

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