L-PRP diminishes bone matrix formation around autogenous bone grafts associated with changes in osteocalcin and PPAR-γ immunoexpression

Portela, Germán; Cerci, D.X.; Pedrotti, G.; Araujo, Melissa Rodrigues de; Deliberador, Tatiana Miranda; Zielak, João César; Costa-Casagrande, Thaís Andrade; Gonzaga, Carla Castiglia; Giovanini, Allan Fernando

doi:10.1016/j.ijom.2013.07.739

Cited by 14 publications

(12 citation statements)

References 41 publications

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“…The rats were kept in a room with a controlled temperature (22 °C) and maintained under a 12-h light-dark cycle. The protocol of PRP production and quantification as well as the surgical procedures performed in this study were based on Portela et al (5) and are described below.…”

Section: Methodsmentioning

confidence: 99%

“…The histomorphometric data were quantified manually and expressed as area observed at 5 mm 2 . The immunopositivity for Erk1/2, p38α/β, CD36 and adiponectin was determined by automation, as described by Portela et al (5). All immunohistochemical results were performed in triplicate and all data were transformed into percentages in order to facilitate their interpretation.…”

Section: Image Analysismentioning

confidence: 99%

“…The favorable use of PRP for osteogenesis has been substantiated on the hypothesis that the platelets produce growth factors that modulate the synthesis and deposition of extracellular matrix (ECM) and angiogenesis, and promotes chemotaxis of osteoprogenitor cells and their differentiation into osteoblasts (3). Nonetheless, despite this remarkable biological action, it has been claimed that the presence of PRP may not only impair osteogenesis, but also produce an abnormal histophenotype during craniofacial bone repair (4,5).…”

Section: Introductionmentioning

confidence: 99%

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Platelet Rich Plasma (PRP) Produces an Atherofibrotic Histophenotype During Craniofacial Bone Repair Due to Changes of Immunohistochemical Expression of Erk1/2, p38α/β, Adiponectin and Elevated Presence of Cells Exhibiting B-scavenger Receptor (CD36+)

Schroeder¹,

Scariot²,

Zielak³

et al. 2016

Braz. Dent. J.

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The platelet-extracellular matrix interaction in platelet rich plasma (PRP) through thrombospondin receptor-CD36 induces the secretion of growth factors responsible for cellular proliferation and differentiation during the repair process. Since CD36 also acts as a class B-scavenger-receptor for development of foam-like cells and mitogen-activated kinases, such as Erk1/2 and p38α/β, are important proteins activated by platelet growth factor, the aim of this study was to evaluate the immunohistochemical presence of CD36, Erk1/2, p38α/β during the bone repair treated and non-treated with PRP and to compare these results with the histomorphometry of repair. Simultaneously, the immunopresence of adiponectin was analyzed, which may contribute to osteogenesis at the same time it inhibits fibrosis and impairs adipogenesis and foam cell formation in the medullary area. An artificial bone defect measuring 5×1 mm was produced in the calvaria of 56 Wistar rats. The defects were randomly treated with autograft, autograft+PRP, PRP alone and sham. The animals were euthanized at 2 and 6 weeks post-surgery. Data were analyzed by ANOVA followed by non-parametric test Student Newman-Keuls (p<0.05) for histomorphometric and immunohistochemical interpretation. The results revealed that in specimens that received PRP the immunopositivity for Erk1/2, p38α/β and CD36 proteins increased significantly while the immunohistochemical expression of adiponectin decreased simultaneously. There was also an accentuated reduction of bone matrix deposition and increase of the medullary area represented by fibrosis and/ or presence of foam-like cells, which exhibited immunophenotype CD36+adiponectin. The findings of this study suggest that PRP acted as an inhibitor of osteogenesis during the craniofacial bone repair and induced a pathological condition that mimics an atherofibrotic condition.

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Section: Methodsmentioning

confidence: 99%

Section: Image Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Platelet Rich Plasma (PRP) Produces an Atherofibrotic Histophenotype During Craniofacial Bone Repair Due to Changes of Immunohistochemical Expression of Erk1/2, p38α/β, Adiponectin and Elevated Presence of Cells Exhibiting B-scavenger Receptor (CD36+)

Schroeder¹,

Scariot²,

Zielak³

et al. 2016

Braz. Dent. J.

View full text Add to dashboard Cite

show abstract

“…This hypothesis seems probable, as there is evidence that the platelet growth factor TGF-β may work as an inhibiting factor on the terminal differentiation of osteoblasts, suppressing the bone morphogenetic-2 protein (BMP2) expression (Spinella-Jaegle et al, 2001;Giovanini et al, 2014) or cross-reacting with Wnt-10b M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPT 4 osteoprotein, which impedes the canonical pathways of osteo-lineage maturation and diminishes bone matrix formation (Giovanini et al, 2013).…”

Section: A N U S C R I P Tmentioning

confidence: 99%

“…Despite the knowledge that the synthesis of some platelet growth factors is expressed during bone metabolism and osteoneogenesis (Marx et al, 1998;Marx, 2004;El Backly et al, 2014), results demonstrating that autograft associated with L-PRP may impair local bone formation have been revealed by several studies (Mooren et al, 2010;Aghaloo et al, 2004;Choi et al, 2004;Giovanini et al, 2010;Giovanini, et al, 2011;Kazakos et al, 2011;Giovanini et al, 2013;Portela et al, 2014).…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Leukocyte-platelet-rich plasma diminishes bone matrix deposition in rat calvaria treated with autograft due to simultaneous increase in immunohistochemical expression of Indian Hedgehog, transforming growth factor-β, and parathyroid-1 receptor

Cerci

Portela

Cunha

et al. 2015

Journal of Cranio-Maxillofacial Surgery

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The roles of cellular and molecular components of a hematoma at early stage of bone healing

Shiu

Leung

2018

J Tissue Eng Regen Med

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Bone healing is a complex repair process that commences with the formation of a blood clot at the injured bone, termed hematoma. It has evidenced that a lack of a stable hematoma causes delayed bone healing or non-union. The hematoma at the injured bone constitutes the early healing microenvironment. It appears to dictate healing pathways that ends in a regenerative bone. However, the hematoma is often clinically removed from the damaged site. Conversely, blood-derived products have been used in bone tissue engineering for treating critical sized defects, including fibrin gels and platelet-rich plasma. A second generation of platelet concentrate that is based on leukocyte and fibrin content has also been developed and introduced in market. Conflicting effect of these products in bone repair are reported. We propose that the bone healing response becomes dysregulated if the blood response and subsequent formation and properties of a hematoma are altered. This review focuses on the central structural, cellular, and molecular components of a fracture hematoma, with a major emphasis on their roles in regulating bone healing mechanism, and their interactions with mesenchymal stem cells. New angles towards a better understanding of these factors and relevant mechanisms involved at the beginning of bone healing may help to clarify limited or adverse effects of blood-derived products on bone repair. We emphasize that the recreation of an early hematoma niche with critical compositions might emerge as a viable therapeutic strategy for enhanced skeletal tissue engineering.

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L-PRP diminishes bone matrix formation around autogenous bone grafts associated with changes in osteocalcin and PPAR-γ immunoexpression

Cited by 14 publications

References 41 publications

Platelet Rich Plasma (PRP) Produces an Atherofibrotic Histophenotype During Craniofacial Bone Repair Due to Changes of Immunohistochemical Expression of Erk1/2, p38α/β, Adiponectin and Elevated Presence of Cells Exhibiting B-scavenger Receptor (CD36+)

Platelet Rich Plasma (PRP) Produces an Atherofibrotic Histophenotype During Craniofacial Bone Repair Due to Changes of Immunohistochemical Expression of Erk1/2, p38α/β, Adiponectin and Elevated Presence of Cells Exhibiting B-scavenger Receptor (CD36+)

Leukocyte-platelet-rich plasma diminishes bone matrix deposition in rat calvaria treated with autograft due to simultaneous increase in immunohistochemical expression of Indian Hedgehog, transforming growth factor-β, and parathyroid-1 receptor

The roles of cellular and molecular components of a hematoma at early stage of bone healing

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