L'opéron : groupe de gènes à expression coordonnée par un opérateur [C. R. Acad. Sci. Paris 250 (1960) 1727–1729]

The use of time series profiling to identify groups of functionally related genes (synexpression groups) is a powerful approach for the discovery of gene function. Here we apply this strategy during RasV12 immortalization of Drosophila embryonic cells, a phenomenon not well characterized. Using high-resolution transcriptional time-series datasets, we generated a gene network based on temporal expression profile similarities. This analysis revealed that common immortalized cells are related to adult muscle precursors (AMPs), a stem cell-like population contributing to adult muscles and sharing properties with vertebrate satellite cells. Remarkably, the immortalized cells retained the capacity for myogenic differentiation when treated with the steroid hormone ecdysone. Further, we validated in vivo the transcription factor CG9650, the ortholog of mammalian Bcl11a/b, as a regulator of AMP proliferation predicted by our analysis. Our study demonstrates the power of time series synexpression analysis to characterize Drosophila embryonic progenitor lines and identify stem/progenitor cell regulators.

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“…GSE73354). 1 To whom correspondence may be addressed. Email: mdequeant@genetics.med.harvard.edu or perrimon@receptor.med.harvard.edu.…”

mentioning

confidence: 99%

Discovery of progenitor cell signatures by time-series synexpression analysis during Drosophila embryonic cell immortalization

Dequéant

Fagegaltier

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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“…The Lac operon is a set of genes that code for proteins which together regulate the transport and metabolism of lactose in prokaryotes like Escherichia coli. It is inducible in the sense that the genes are normally not expressed until a small molecule (like lactose or MTG) binds to a DNA-binding protein and induces the synthesis of the regulated proteins [43]. This genetic construct has been adapted to other organisms beyond E. coli and it is routinely used to achieve inducible gene expression in animals ranging from fruit flies [44] to mice [45].…”

Section: Ru-bpy Complexes Poor Donor Ligands and Genetic Inducersmentioning

confidence: 99%

Ruthenium polypyridyl phototriggers: from beginnings to perspectives

Zayat

Filevich

Baraldo

et al. 2013

Phil. Trans. R. Soc. A.

104

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Octahedral Ru(II) polypyridyl complexes constitute a superb platform to devise photoactive triggers capable of delivering entire molecules in a reliable, fast, efficient and clean way. Ruthenium coordination chemistry opens the way to caging a wide range of molecules, such as amino acids, nucleotides, neurotransmitters, fluorescent probes and genetic inducers. Contrary to other phototriggers, these Ru-based caged compounds are active with visible light, and can be photolysed even at 532 nm (green), enabling the use of simple and inexpensive equipment. These compounds are also active in the two-photon regime, a property that extends their scope to systems where IR light must be used to achieve high precision and penetrability. The state of the art and the future of ruthenium polypyridyl phototriggers are discussed, and several new applications are presented.

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“…After Art's lab moved from Berkeley to Princeton University, he came up with an idea that was inspired by the Pasteur group's characterization of the lac operator. As initially conceived by Jacob and Monod, the operator, located at the beginning of the operon, served both as the site where transcription of the lac operon began and the site where the repressor acted to block this initiation (6). This model was based on two classes of mutations: operator-constitutive mutations (O c ) that were resistant to repression and O o mutations that mapped early in the operon and eliminated the activity of both genes of the operon, lacZ and lacY.…”

Section: Suppressors Lac Operon Regulation and Translation Terminationmentioning

confidence: 99%

Genetic Suppressors and Recovery of Repressed Biochemical Memory

Beckwith

2009

Journal of Biological Chemistry

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L'opéron : groupe de gènes à expression coordonnée par un opérateur [C. R. Acad. Sci. Paris 250 (1960) 1727–1729]

Abstract: Fac-similé de la note / Facsimile of the article L'opéron : groupe de gènes à expression coordonnée par un opérateur [C. R. Acad. Sci.

Cited by 80 publications

References 2 publications

Discovery of progenitor cell signatures by time-series synexpression analysis during Drosophila embryonic cell immortalization

Discovery of progenitor cell signatures by time-series synexpression analysis during Drosophila embryonic cell immortalization

Ruthenium polypyridyl phototriggers: from beginnings to perspectives

Genetic Suppressors and Recovery of Repressed Biochemical Memory

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