L- MYC Expression Maintains Self-Renewal and Prolongs Multipotency of Primary Human Neural Stem Cells

Li, Zhongqi; Oganesyan, Diana; Mooney, Rachael; Xiao, Rong; Christensen, Matthew J.; Shahmanyan, David; Perrigue, Patrick M.; Benetatos, Joseph; Tsaturyan, Lusine; Aramburo, Soraya; Annala, Alexander J.; Lü, Yang; Najbauer, Joseph; Wu, Xiwei; Barish, Michael E.; Brody, David L.; Aboody, Karen S.; Gutova, Margarita

doi:10.1016/j.stemcr.2016.07.013

Cited by 18 publications

(28 citation statements)

References 38 publications

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“…The ReNcell CX cells grew rapidly as a monolayer on laminin, with a doubling time of ~24 h due to the c-MYC-based immortalization. Therefore, there are safety concerns, including the risk that oncogenic c-MYC may render this hNSC line tumorigenic following transplantation ( 19 , 25 ).…”

Section: Resultsmentioning

confidence: 99%

Comparative analysis of human embryonic stem cell‑derived neural stem cells as an in vitro human model

Jung

Lee

et al. 2017

Int J Mol Med

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Alternative cell models of human neural stem cells (hNSCs) have been developed and used for investigations ranging from in vitro experiments to in vivo clinical studies. However, a cell model capable of mimicking the ʻnormalʼ state of hNSCs is mandatory in order to extrapolate the results of these studies to humans. In the present study, to select a more suitable hNSC model for developing human-based experimental platforms, two representative hNSC types were compared, namely human embryonic stem cell (hESC)-derived hNSCs and ReNcell CX cells, which are well-characterized immortalized hNSC lines. The hNSCs, differentiated from hESCs via human neuroectodermal sphere (hNES) formation, recapitulated the molecular and cellular phenotypes of hNSCs, including NSC marker expression and terminal neuronal differentiation potential. Comparative analyses of the transcriptome profiles of the hESC-derived hNESs and ReNcell CX hNSCs showed that the differentiated hNESs were analogous to the ReNcell CX cells, as demonstrated by principal component analysis and hierarchical sample clustering. The hNSC-specific transcriptome was presented, comprising commonly expressed transcripts between hNESs derived from hESCs and ReNcell CX cells. To elucidate the molecular mechanisms associated with the hNSC identity, the hNSC-specific transcriptome was analyzed using pathway and functional annotation clustering analyses. The results suggested that hESC-derived hNESs, an expandable and accessible cell source, may be used as a relevant hNSC model in a wide range of neurological investigations.

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Section: Resultsmentioning

confidence: 99%

Comparative analysis of human embryonic stem cell‑derived neural stem cells as an in vitro human model

Jung

Lee

et al. 2017

Int J Mol Med

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“…Differentiation of TS lines was performed by withdraw of EGF/FGF in the TS culture media and supplement with 10% fetal calf serum (FCS), as depicted previously (16). FB-NSCs were established and characterized as reported in previous studies (52)(53)(54). Astrocytes and NPCs are differentiated from health donor-derived iPSCs based on established protocols (85,86).…”

Section: Isolation Of Primary Brain Tumor Cells Establishment Of Neumentioning

confidence: 99%

Chlorotoxin Redirects Chimeric Antigen Receptor T Cells for Specific and Effective Targeting of Glioblastoma

Wang

Starr

Chang

et al. 2020

Preprint

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One Sentence Summary: Chimeric antigen receptors incorporating chlorotoxin as the tumor targeting domain recognize and kill glioblastoma with high specificity and potency. AbstractWhile chimeric antigen receptor (CAR) T cells have demonstrated antitumor activity against glioblastoma (GBM), tumor heterogeneity remains a critical challenge. To more effectively target heterogeneous GBMs, we report the development of a novel peptide-based CAR exploiting the GBM-binding potential of chlorotoxin (CLTX). CLTX bound a greater proportion of tumor cells than GBM-associated antigens

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“…Combination treatment with HDAC and lysine-specific histone demethylase (LSD)-1 inhibitors resulted in synergistic apoptosis in glioblastoma cells but not normal astrocytes (Singh, Manton et al 2011). Alternatively, drugs preventing L-MYC (Li, Oganesyan et al 2016), HMGA-2 (Zhong, Liu et al 2016), fatty acid synthase (FASN) (Yasumoto, Miyazaki et al 2016), astrocyte elevated gene-1 (AEG-1) (Hu, Emdad et al 2016), and lysine demethylase KDM1A (Sareddy, Viswanadhapalli et al 2016) activity and promoting PAX6/DLX5 transcriptional activation of WNT signaling (Hu, Wang et al 2016) prevented glioma stem cell morphology and induced astrocytic differentiation. These preclinical studies suggest that eliminating the glioblastoma stem cell (GBSc) population and inducing astrocytic differentiation of the glioma cells may be a viable selective treatment strategy to explore for BTSCs, although further testing is needed in an in vivo systems.…”

Section: Differentiationmentioning

confidence: 99%

Brain Tumor Treatment: 2017 Update

Chakraborty¹,

Han²,

Faltas³

et al. 2018

CCO

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Malignant brain tumors are a heterogeneous group of diseases arising from different cell types that affect both adults and children. The high recurrence rate of malignant brain tumors typically is due to reappearance of focal masses, indicating that a sub population of tumor cells are insensitive to current therapies and may be responsible for reinitiating tumor growth. It is generally agreed that the resistant tumor cells are comprised of cancer stem cells or tumor-initiating cells. While brain tumor stem cells (BTSCs) were first isolated within the last decade, much of the early research has been focused on identifying the BTSC markers and therapeutic targets. The challenge however, is to translate this knowledge to therapeutics. In the current review, we survey the remedial strategies to target BTSCs, which includes diagnostic, pharmacologic, immunologic, viral, and post-transcriptional approaches.

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L- MYC Expression Maintains Self-Renewal and Prolongs Multipotency of Primary Human Neural Stem Cells

Cited by 18 publications

References 38 publications

Comparative analysis of human embryonic stem cell‑derived neural stem cells as an in vitro human model

Comparative analysis of human embryonic stem cell‑derived neural stem cells as an in vitro human model

Chlorotoxin Redirects Chimeric Antigen Receptor T Cells for Specific and Effective Targeting of Glioblastoma

Brain Tumor Treatment: 2017 Update

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