L-lysine-.alpha.-ketoglutarate aminotransferase. I. Identification of a product, .DELTA.1-piperideine-6-carboxylic acid

Biotransformation ofL-Lysine toL-Pipecolic Acid Catalyzed byL-Lysine 6-Aminotransferase and Pyrroline-5-carboxylate Reductase

“…2A). Since LAT catalyzes the conversion of L-Lys to P6C, 6,10) this result implies that there is a gene encoding another`P 6C reductase'' also in the genome of E. coli JM109.…”

“…4) In Pseudomonas putida, L-Lys is initially converted to D-lysine, metabolized to P2C, and then P2C is converted to L-PA by P2C reductase. 5) P6C, which is the reversible cyclic form of a-aminoadipic semialdehyde, was identiˆed as the product of the deamination of L-Lys that was catalyzed by L-lysine 6-aminotransferase (LAT, E.C.2.6.1.36) in Flavobacterium lutescens IFO3084 6) and b-lactam-producing actinomycetes 7) (Fig. 1).…”

Identification by Heterologous Expression and Gene Disruption of VisA as l -Lysine 2-Aminotransferase Essential for Virginiamycin S Biosynthesis in Streptomyces virginiae

“…The lysine 2-aminotransferase activity was assayed as described by Soda et al (19). The standard incubation mixture contained protein (up to 150 l), 5 mM L-lysine, 100 mM 2-oxobutyrate, 70 M pyridoxal 5Ј-phosphate (PLP), and 100 M potassium phosphate (pH 8.0) in a final volume of 250 l. The reaction was initiated by the addition of the protein and the mixture was incubated for 20 min at 30°C followed by the addition of a 50% trichloroacetic acid-absolute ethanol (1:10) mixture (125 l) to terminate the reaction.…”

“…by monitoring the colored complex formation between the enzymatic product and o-aminobenzaldehyde according to the method of Soda et al (19). The crude extract showed clear activity of 0.07 U/mg of protein, whereas no activity (Ͻ0.0001 U/mg) was detected with control extracts prepared from E. coli cells harboring pET32a(ϩ) alone.…”

Molecular characterization of lysine 6-dehydrogenase from Achromobacter denitrificans

“…However, it is not clear which amino group of L-lysine the enzyme cleaves because the reaction product from L-lysine was only examined via thin layer chromatography. By using this chromatographic technique, delta-1-piperideine-2-carboxylate (P-2-C), the product of the Lys 2-DH reaction, was not separated from P-6-C (15 ). This paper documents the isolation of a new Lys 6-DH producer, Achromobacter denitrificans.…”