L Forms of Streptococcus Fecalis in Septicemia

Mattman, Lida H.

doi:10.1001/archinte.1965.03860150059011

Cited by 37 publications

(7 citation statements)

References 12 publications

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“…Recently much research has been done in an attempt to discover the possible role of the L-phase in the cause of disease and especially in the relapses of chronic infections (5). L-phase organisms have actually been isolated from many cases of chronic staphylococcal infections (6), septicemias (17,18), chronic bacteriuria (9), meningitis (13,17), tuberculosis (17,19), rheumatic fever and endocarditis (7,11,13), chronic skin and mucosal lesions (6,8), and pyelonephritis (15). Some diseases, for example, meningitis and meningoencephalitis (13), pyelonephritis (10), and chronic skin lesions (8), have been produced in laboratory animals by inoculation of L-phase organisms.…”

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confidence: 99%

“…The bacterial phase was incubated in Brain Heart Infusion broth (BHI, Difco) with 20% sucrose for 18 hr, and the L-phase was produced by inoculating 0.5 ml of the bacterial broth culture into 10 twofold dilution tubes containing 0.5 ml of penicillin (potassium penicillin G, Squibb) in the range of 100 to 0.2 units/ml. The penicillin was also diluted in a solution of 20% sucrose in BHI broth.…”

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Selection of Media for the Isolation of Common Bacterial L-Phase Organisms from a Clinical Specimen

Nimmo¹,

Blazevic²

1969

Applied Microbiology

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The L-phase of 13 bacteria commonly associated with disease were induced by penicillin and inoculated into various solid and broth media; their growth was recorded for a period of 14 days. Plates containing highly purified agar and sucrose as the stabilizing agent and those incubated under aerobic conditions gave the best results. Magnesium seems to be necessary for growth in broth media on primary isolation, although it may not be necessary on multiple transfers after a more stable state has been reached. Growth in broth media is much more difficult to achieve. Reversion is aided by using a higher concentration of agar in plates, by decreasing the sucrose concentration, and by omitting the antibiotics and horse serum. A procedure has been outlined for the routine culture and identification of L-phase organisms from a clinical specimen. MATERIALS AND METHODS This study made use of 13 strains of bacteria sensitive to penicillin in the range of 1 to 25 units/ml and included 3 strains of Escherichia coli, 2 strains of Proteus mirabilis, 1 Klebsiella, 2 strains, each of coagulase-positive and coagulase-negative staphylococci, 2 strains of enterococci, and 1 beta-hemolytic streptococcus. These organisms were selected because of their availability and their high incidence in infection, and because they represent approximately equal numbers of gram-negative rods and grampositive cocci.

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Selection of Media for the Isolation of Common Bacterial L-Phase Organisms from a Clinical Specimen

Nimmo¹,

Blazevic²

1969

Applied Microbiology

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“…Unusual organisms such as Brucella, Listeria, and Rickettsia have been isolated, and in one case L forms of Strep. faecalis were incriminated (Hudson, 1957;Mattman and Mattman, 1965;Ferguson, Craik, and Grist, 1962;Hoeprich and Chernoff, 1955). Failure to provide specialized growth requirements may account for some negative cultures.…”

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Bacterial endocarditis 1956-1965: analysis of clinical features and treatment in relation to prognosis and mortality.

Shinebourne¹,

Cripps²,

Hayward³

et al. 1969

Heart

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“…A high number of all blood cultures submitted to bacteriology laboratories are negative. However, more positives are found when CWD bacteria are sought (1,4,7,8,12); sometimes the increase is at least threefold (10). Therefore, early detection of the CWD variants is of practical importance, since the seriousness of septicemia necessitates a prompt clue as to the infectious state.…”

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Structures Suggesting Cell-Wall-Deficient Forms Detected in Circulating Erythrocytes by Fluorochrome Staining

Pohlod¹,

Mattman²,

Tunstall³

1972

Applied Microbiology

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Cell-wall-deficient (CWD) forms of bacteria are associated with certain cases of idiopathic septicemia. In this preliminary study of blood examined immediately after venipuncture, structures with a morphology characteristic of CWD forms were seen parasitizing the erythrocytes. These inclusions were usually circumferential, but in some cases they protruded from the red cells. The CWD forms were detected by staining with Gould's rhodamine-labeled muramidase, which reacted similarly to acridine orange but with greater specificity. A blocking test, employing unlabeled muramidase, indicated the specificity of the reaction between muramidase and the microbial substrate. Reaction of the forms with muramidase indicates their bacterial, rather than mycoplasmal, nature. Thus in vivo CWD forms have a detectable component of muramic acid, at least in certain cases. Sixty-eight individuals with a diagnosis of fever of unknown origin were tested, with 51 nondebilitated individuals serving as controls. More intraerythrocytic forms reacting with muramidase were found in the patients than in the controls. Nearly 40% of the cases had a relatively high incidence of erythrocyte parasitism. In some instances when freshly drawn blood was examined, the structures, which appear to be microbial, extended in rhizoid filaments from the erythrocytes.

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L Forms of Streptococcus Fecalis in Septicemia

Cited by 37 publications

References 12 publications

Selection of Media for the Isolation of Common Bacterial L-Phase Organisms from a Clinical Specimen

Selection of Media for the Isolation of Common Bacterial L-Phase Organisms from a Clinical Specimen

Bacterial endocarditis 1956-1965: analysis of clinical features and treatment in relation to prognosis and mortality.

Structures Suggesting Cell-Wall-Deficient Forms Detected in Circulating Erythrocytes by Fluorochrome Staining

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