L-form bacteria of Pseudomonas syringae pv. phaseolicola induce chitinases and enhance resistance to Botrytis cinerea infection in Chinese cabbage

Daulagala, P. W. H. K. P.; Allan, Eunice J.

doi:10.1016/s0885-5765(03)00089-4

Cited by 15 publications

(20 citation statements)

References 33 publications

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“…Cette spécificité variétale a été attribuée à des différences histologiques (présence ou absence de pores, structure et épaisseur de l'épiderme et de la cuticule) et biochimiques (synthèse de phytoalexines, proanthocyanidines …) (Gabler et al, 2003). Elle pourrait être également due aux différences dans les potentiels des variétés à produire les protéines PR, notamment les chitinases et β-1,3-glucanases (Daulaga et Allan, 2003). De même, cette différence est remarquée entre les souches isolées à partir de différentes parties végétales de la même variété de tomate, ce qui serait dû à une adaptation de chaque pathotype, par amélioration de ses moyens d'infection, à un type d'organe déterminé.…”

Section: Resultatsunclassified

Mise en évidence d’une variation intra spécifique chez Botrytis cinerea et lutte biologique in vitro par l’extrait de compost

Mouria¹,

Ouazzani-Touhami²,

Mouria³

et al. 2013

J. App. Bioscience.

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Detection of Intraspecific variation in Botrytis cinerea and in vitro biological control by compost extract.Objectives: The present study was on intraspecific variation detection in B. cinerea and the effect of a municipal solid waste compost extract on two stages of its life cycle. Methodology and results: tomato leaves were inoculated with 22 strains of B. cinerea to select the most virulent. They were transplanted into culture medium based on compost extract to assess the growth and sporulation. B. cinerea strains isolated from tomato plants proved to be more virulent. Growth and sporulation of five strains selected were inhibited by the extracts in the media by 95.24% and 100% respectively. Conclusion and application of research: The study of pathogenicity of B. cinerea showed the existence of intraspecific variation. The sterilized compost extracts efficiency on B. cinerea in vitro proves that chemical compounds are involved and suggests the possibility of using these extracts and compost in vivo against tomato gray mold. Mouria et al.… J. Appl. Biosci. 2013. Mise en évidence d'une variation intraspecific chez B. cinerea et ce lutte biologique in vitro.

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Section: Resultatsunclassified

Mise en évidence d’une variation intra spécifique chez Botrytis cinerea et lutte biologique in vitro par l’extrait de compost

Mouria¹,

Ouazzani-Touhami²,

Mouria³

et al. 2013

J. App. Bioscience.

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“…In plants, artificially generated L‐forms of bacteria have been shown to readily enter seedling root tissues and establish intracellular symbiotic growth (Amijee et al, ; Daulagala and Allan, ). In several plants, establishment of endosymbiotic L‐forms of bacteria resulted in enhanced resistance to a range of plant pathogens, although the mechanism of enhanced resistance has not been clarified (Amijee et al, ).…”

Section: Discussionmentioning

confidence: 99%

Hydrogen peroxide staining to visualize intracellular bacterial infections of seedling root cells

White

Torres

Somu

et al. 2014

Microscopy Res & Technique

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Visualization of bacteria in living plant cells and tissues is often problematic due to lack of stains that pass through living plant cell membranes and selectively stain bacterial cells. In this article, we report the use of 3,3'-diaminobenzidine tetrachloride (DAB) to stain hydrogen peroxide associated with bacterial invasion of eukaryotic cells. Tissues were counterstained with aniline blue/lactophenol to stain protein in bacterial cells. Using this staining method to visualize intracellular bacterial (Burkholderia gladioli) colonization of seedling roots of switch grass (Panicum virgatum), we compared bacterial free seedling roots and those inoculated with the bacterium. To further assess application of the technique in multiple species of vascular plants, we examined vascular plants for seedling root colonization by naturally occurring seed-transmitted bacteria. Colonization by bacteria was only observed to occur within epidermal (including root hairs) and cortical cells of root tissues, suggesting that bacteria may not be penetrating deeply into root tissues. DAB/peroxidase with counter stain aniline blue/lactophenol was effective in penetration of root cells to selectively stain bacteria. Furthermore, this stain combination permitted the visualization of the bacterial lysis process. Before any evidence of H2 O2 staining, intracellular bacteria were seen to stain blue for protein content with aniline blue/lactophenol. After H2 O2 staining became evident, bacteria were often swollen, without internal staining by aniline blue/lactophenol; this suggests loss of protein content. This staining method was effective for seedling root tissues; however, it was not effective at staining bacteria in shoot tissues due to poor penetration.

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“…AM50 towards the plant pathogens P. capsici, C. gloeosporioides, and R. solani. Various studies and case reports (Takahashi et al, 1993;Chang et al, 2001;Hassane et al, 2001;Wichitra et al, 2002;Daulagala and Allan, 2003) have already investigated the potent antifungal activity of Streptomyces sp., which are saprophytic soil bacteria (Akihiro et al, 1998), the major producers of chitinase in soil, and use chitin as their source of carbon. Increased concern over the impact of chemical pesticides on the environment has resulted in the increased interest in biocontrol strategies for the management of the plant pathogens.…”

Section: Discussionmentioning

confidence: 99%

Characterization and Antifungal Activity from Soilborne Streptomyces sp. AM50 towards Major Plant Pathogens

Jang¹,

Lee²,

Kim³

et al. 2011

Korean Journal of Environmental Agriculture

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BACKGROUND:Chemical fungicides not only may pollute the ecosystem but also can be environmentally hazardous, as the chemicals accumulate in soil. Biological control is a frequently-used environment-friendly alternative to chemical pesticides in phytopathogen management. However, the use of microbial products as fungicides has limitations. This study isolated and characterized a three-antifungal-enzyme (chitinase, cellulase, and β-1,3-glucanase)-producing bacterium, and examined the conditions required to optimize the production of the antifungal enzymes. METHOD AND RESULTS:The antifungal enzymes

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L-form bacteria of Pseudomonas syringae pv. phaseolicola induce chitinases and enhance resistance to Botrytis cinerea infection in Chinese cabbage

Cited by 15 publications

References 33 publications

Mise en évidence d’une variation intra spécifique chez <i>Botrytis cinerea</i> et lutte biologique <i>in vitro</i> par l’extrait de compost

Mise en évidence d’une variation intra spécifique chez <i>Botrytis cinerea</i> et lutte biologique <i>in vitro</i> par l’extrait de compost

Hydrogen peroxide staining to visualize intracellular bacterial infections of seedling root cells

Characterization and Antifungal Activity from Soilborne Streptomyces sp. AM50 towards Major Plant Pathogens

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