l-carnosine induces apoptosis/cell cycle arrest via suppression of NF-κB/STAT1 pathway in HCT116 colorectal cancer cells

Lee, Joo-Yeon; Park, Jeong-Ran; Lee, Hanbyeol; Jang, Soojin; Ryu, Se-Min; Kim, Hye-Jin; Kim, Donguk; Jang, Aera; Yang, Se‐Ran

doi:10.1007/s11626-018-0264-4

Cited by 23 publications

(24 citation statements)

References 28 publications

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“…tified role of carnosine occurring during digestion with a likelihood of protection first for the GIT itself considering among others the reported antiproliferative effect of carnosine on gastric and colorectal cancer cells(Cheng et al, 2019;Lee et al, 2018). The results, in addition, raise the possibility that the effects of carnosine in the GIT could potentially contribute further outside the GIT.…”

mentioning

confidence: 81%

Protective effects of dietary carnosine during in‐vitro digestion of pork differing in fat content and cooking conditions

Yaylayan

Palin

et al. 2021

J. Food Biochem.

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Muscle carnosine represents an important health advantage of meat. Ground pork samples with intrinsic or added carnosine; fat content; and cooked under low or high intensity as a 2 × 2 × 2 factorial were digested in‐vitro. Changes in free carnosine and in markers of lipid (hexanal, 4‐hydroxynonenal (4‐HNE), malondialdehyde (MDA) and protein (protein‐carbonyls, thiols) oxidation, and of advanced glycation end‐products (AGEs) Nε‐(carboxymethyl)lysine (CML) were determined in the saliva, gastric, and duodenal digests. During digestion, the different markers overall indicated increased oxidation and decreased free carnosine. Increasing pork carnosine level significantly reduced protein carbonyls, loss of thiols, and 4‐HNE during in‐vitro gastric digestion, irrespective of fat and cooking level of the meat. Increased carnosine also significantly reduced hexanal, MDA and CML up to the duodenum phase in moderately cooked lean pork. Besides substantiating the formation of AGEs during digestion, these results show a potentially important role of dietary carnosine occurring in the gastrointestinal tract. Practical applications The ailments epidemiologically associated with red meat consumption could be counteracted by ingesting carnosine into meat. The health advantages of dietary carnosine, however, have never been demonstrated during digestion, a unique and complex oxidative environment compounded by the composition and cooking of the meat. The results obtained substantiated that AGEs formation occurred in‐vitro in the GIT. They also showed that increased carnosine had an immediate health beneficial role during pork digestion in reducing the formation of different harmful molecules, including AGEs, modulated by the composition and cooking of the meat. However, in exerting this protective role in the GIT, the remaining free level of carnosine, gradually decreased during digestion. Carnosine, as an important meat compositional factor may, depending on the fat content and cooking conditions, change the image of meat from representing a health risk to a health benefit. Carnosine level may also explain discrepancies observed in the literature.

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mentioning

confidence: 81%

Protective effects of dietary carnosine during in‐vitro digestion of pork differing in fat content and cooking conditions

Yaylayan

Palin

et al. 2021

J. Food Biochem.

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“…There are only few studies reporting some possible mechanisms of the effects of CAR on the cell cycle of cancer cells, and these do not mention if the effects on the cell cycle were the result of adding CAR to the growth culture media at a single time point or at several times during cell culture 31,33,46 . No information is available regarding the effects of CAR on cell cycle progression of normal cells.…”

Section: Discussionmentioning

confidence: 99%

“…CAR also reduced the extracellular acidification rate and glycolysis 32 . CAR induced apoptosis/cell cycle arrest in human colorectal cancer cells by suppressing of NF-κB/STAT1 signaling 33 .…”

Section: Introductionmentioning

confidence: 98%

Radio-selective effects of a natural occurring muscle-derived dipeptide in A549 and normal cell lines

Ybarra

Seuntjens

2019

Sci Rep

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Radiotherapy (RT) causes morbidity and long-term side effects. A challenge in RT is to maximize cancer cells killing while minimizing damage to normal tissue. The ideal radio-protector selectively improves survival and limits damage to normal tissues while reducing survival of cancer cells. Muscle-derived dipeptide, L-carnosine (CAR) is a potent antioxidant, with radio-protective, but also anticancer properties, affecting the cell cycle of cancer cells. We tested CAR effects in lung cancer cells, differentiated and undifferentiated normal cells. We hypothesized that CAR antioxidant properties will confer protection to the two normal cell lines against RT, while preventing lung cancer cell proliferation, and that CAR may act as a radiosensitizer of lung cancer cells due to its effects on cell-cycle progression of cancer cells. Under the experimental conditions reported here, we found that CAR increased radio-sensitivity of lung (A549) cancer cells by increasing the percentage of cells in G2/M (radiosensitive) phase of cell cycle, it negatively affected their bioenergetics, therefore reduced their viability, and DNA-double strand break repair capacity. CAR had either no effect or reduced RT-induced damage in normal cells, depending on the cell type. CAR is a versatile natural occurring compound, that could improve RT-induced lung cancer cells killing, while reducing the damage to normal differentiated and undifferentiated cells.

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“…Similarly, Antognelli et al have demonstrated that AG treatment in ATC cell line is able to revert the pro-tumorigenic role of MGO and this effect is enhanced when AG is used in combination with resveratrol, a Glo1 activator (46). L-Carnosine (b-alanyl-L-histidine), a naturally occurring dipeptide acting as MGO scavenger, has been shown to exert antiproliferative effects in cancer cells (113,114). It reverses MGO protumorigenic action by decreasing migratory ability of breast cancer cells (43,67) and inducing apoptosis in CRC cells, both in vitro and in vivo (44).…”

Section: Therapeutic Strategiesmentioning

confidence: 99%

The Dual-Role of Methylglyoxal in Tumor Progression – Novel Therapeutic Approaches

et al. 2021

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One of the hallmarks of cancer cells is their metabolic reprogramming, which includes the preference for the use of anaerobic glycolysis to produce energy, even in presence of normal oxygen levels. This phenomenon, known as “Warburg effect”, leads to the increased production of reactive intermediates. Among these Methylglyoxal (MGO), a reactive dicarbonyl known as the major precursor of the advanced glycated end products (AGEs), is attracting great attention. It has been well established that endogenous MGO levels are increased in several types of cancer, however the MGO contribution in tumor progression is still debated. Although an anti-cancer role was initially attributed to MGO due to its cytotoxicity, emerging evidence has highlighted its pro-tumorigenic role in several types of cancer. These apparently conflicting results are explained by the hormetic potential of MGO, in which lower doses of MGO are able to establish an adaptive response in cancer cells while higher doses cause cellular apoptosis. Therefore, the extent of MGO accumulation and the tumor context are crucial to establish MGO contribution to cancer progression. Several therapeutic approaches have been proposed and are currently under investigation to inhibit the pro-tumorigenic action of MGO. In this review, we provide an overview of the early and latest evidence regarding the role of MGO in cancer, in order to define its contribution in tumor progression, and the therapeutic strategies aimed to counteract the tumor growth.

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l-carnosine induces apoptosis/cell cycle arrest via suppression of NF-κB/STAT1 pathway in HCT116 colorectal cancer cells

Cited by 23 publications

References 28 publications

Protective effects of dietary carnosine during in‐vitro digestion of pork differing in fat content and cooking conditions

Protective effects of dietary carnosine during in‐vitro digestion of pork differing in fat content and cooking conditions

Radio-selective effects of a natural occurring muscle-derived dipeptide in A549 and normal cell lines

The Dual-Role of Methylglyoxal in Tumor Progression – Novel Therapeutic Approaches

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