l -carnitine supplementation during vitrification or warming of in vivo -produced ovine embryos does not affect embryonic survival rates, but alters CrAT and PRDX1 expression

Saraiva, H. F. R. A.; Batista, Ríbrio Ivan Tavares Pereira; Alfradique, V. A. P.; Pinto, Pedro Henrique Nicolau; Ribeiro, Lilian dos Santos; Oliveira, C. S.; Souza-Fabjan, J. M. G.; Camargo, L. S. A.; Fonseca, J. F. da; Brandão, Felipe Zandonadi

doi:10.1016/j.theriogenology.2017.09.022

Cited by 18 publications

(10 citation statements)

References 41 publications

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“…Additionally, in the culture media used for the in vitro embryo production, BSA and FBS were added, which have been associated with a reduction of embryonic cryotolerance by stimulating the formation of lipid droplets [24][25][26][27]. Also, in our work, we used l-carnitine at different concentrations, during in vitro maturation, late in vitro culture (after 48 h) together and separately, with no effect on embryonic cryotolerance in agreement with reports by Phongnimitr et al who used 0.6 mg/mL l-carnitine during maturation [14], Sprícigo et al where 3.03 mM l-carnitine was added to the in vitro maturation of calf oocytes [7], Saravia et al where 3.72 mM (0.06 mg/mL) l-carnitine was added (Sigma C0158 inner salt) to the vitrification solutions for bovine embryos [28], and Zolini et al who used 3.03 mM l-carnitine during in vitro maturation and 0.75, 1.5 and 3.03 mM during the in vitro culture.…”

Section: Discussionsupporting

confidence: 84%

l-Carnitine Supplementation during In Vitro Maturation and In Vitro Culture Does not Affect the Survival Rates after Vitrification and Warming but Alters Inf-T and ptgs2 Gene Expression

Carrillo-González

Rodríguez-Osorio

Long

et al. 2020

IJMS

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l-carnitine is a potent antioxidant used for in vitro culture systems. Controversial results have been reported using l-carnitine in culture medium at different stages of in vitro bovine embryo production. Cumulus-oocyte complexes (n = 843) were in vitro-fertilized and cultured and added (treatment group) or not added (control group) with l-carnitine. At day three of culture, each group was subdivided into two subgroups receiving no l-carnitine (group 1), 3.8 mM l-carnitine added during in vitro maturation (group 2), 1.5 mM added during the in vitro culture (group 3), and 3.8 mM and 1.5 mM added during the maturation and culture, respectively (group 4). At day 8, blastocyst embryos were examined for mitochondrial activity, the presence of lipid droplets, total cell number, gene expression, and cryotolerance by vitrification. The data were analyzed with a one-way analysis of variance. l-carnitine added in the late in vitro culture significantly reduced mitochondrial activity and lipid content, and upregulated ifn-τ and ptgs2 gene expression compared to controls (p < 0.05). l-carnitine supplementation did not significantly affect the embryo rate production or survival rate after vitrification and warming (p > 0.05). l-carnitine supplementation significantly improved embryo potential to develop viable pregnancies in agreement with a study reporting improved pregnancy rates.

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Section: Discussionsupporting

confidence: 84%

l-Carnitine Supplementation during In Vitro Maturation and In Vitro Culture Does not Affect the Survival Rates after Vitrification and Warming but Alters Inf-T and ptgs2 Gene Expression

Carrillo-González

Rodríguez-Osorio

Long

et al. 2020

IJMS

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“…Interruption of CPT1 is frequently associated with HFD induced metabolic dysfunction and diabetes [52,53]. Furthermore, the function of CPT1 is directly linked to the endogenous levels of L-carnitine, thus it is highly likely to be affected when L-carnitine levels change [54]. In the current study, CPT1b (skeletal muscle isoform) expression levels remarkably elevated in HFD-treated animals, while HBO treatment returned it to normal levels.…”

Section: L-carnitine and Pparα/cpt1bmentioning

confidence: 46%

L-Carnitine Is Involved in Hyperbaric Oxygen-Mediated Therapeutic Effects in High Fat Diet-Induced Lipid Metabolism Dysfunction

et al. 2020

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Lipid metabolism dysfunction and obesity are serious health issues to human beings. The current study investigated the effects of hyperbaric oxygen (HBO) against high fat diet (HFD)-induced lipid metabolism dysfunction and the roles of L-carnitine. C57/B6 mice were fed with HFD or normal chew diet, with or without HBO treatment. Histopathological methods were used to assess the adipose tissues, serum free fatty acid (FFA) levels were assessed with enzymatic methods, and the endogenous circulation and skeletal muscle L-carnitine levels were assessed with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Additionally, western blotting was used to assess the expression levels of PPARα, CPT1b, pHSL/HSL, and UCP1. HFD treatment increased body/adipose tissue weight, serum FFA levels, circulation L-carnitines and decreased skeletal muscle L-carnitine levels, while HBO treatment alleviated such changes. Moreover, HFD treatment increased fatty acid deposition in adipose tissues and decreased the expression of HSL, while HBO treatment alleviated such changes. Additionally, HFD treatment decreased the expression levels of PPARα and increased those of CPT1b in skeletal muscle, while HBO treatment effectively reverted such changes as well. In brown adipose tissues, HFD increased the expression of UCP1 and the phosphorylation of HSL, which was abolished by HBO treatment as well. In summary, HBO treatment may alleviate HFD-induced fatty acid metabolism dysfunction in C57/B6 mice, which seems to be associated with circulation and skeletal muscle L-carnitine levels and PPARα expression.

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“…The main reason for molecular and structural damage during cryopreservation has been reported to be the oxidative stress generation. On the other hand, LC induced cellular responses that ultimately lead to improving energy homeostasis and cell antioxidant defence (Saraiva et al, 2018). Our results showed that treatment of DOs with LC during vitrification and IVM procedures improved the GSH contents and percentage of 2‐cell stage embryos and reduced ROS levels compared with untreated vitrified‐warmed DOs.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, treatment of BCB + oocytes with LC has increased the blastocyst development rate after in vitro fertilization (IVF) via enhanced the expression of genes involved in embryonic development (Zare et al, 2017). Literature reports on the effects of LC treatment on cryotolerance of oocytes and embryos are inconsistent, with authors showing positive effects (Moawad et al, 2013; Truong & Gardner, 2020) or no effect (Carrillo‐González et al, 2020; Saraiva et al, 2018). The current study was designed to determine whether vitrification process is more successful when accomplished on competent COCs or competent DOs.…”

Section: Introductionmentioning

confidence: 99%

Treatment of mouse cumulus‐oocyte complexes with L‐carnitine during vitrification and in vitro maturation affects maturation and embryonic developmental rate after parthenogenetic activation

Zare

Rezaei

Mohammadi

2021

Anat Histol Embryol

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Oocyte vitrification has significantly improved infertility treatment and reproductive biology. This technique provides an opportunity to maintain fertility in patients with premature ovarian failure, women who need gonadotropin treatment, and cancer patients or women who tend to delay motherhood. In addition, oocyte vitrification facilitates oocyte donation programs, thus could be a viable option to maintain fertility in infertile couples who are unable to benefit from embryo cryopreservation techniques, for moral, religious, and legal reasons (Chen, 1986; Prentice & Anzar, 2010;Son et al., 2019).

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l -carnitine supplementation during vitrification or warming of in vivo -produced ovine embryos does not affect embryonic survival rates, but alters CrAT and PRDX1 expression

Cited by 18 publications

References 41 publications

l-Carnitine Supplementation during In Vitro Maturation and In Vitro Culture Does not Affect the Survival Rates after Vitrification and Warming but Alters Inf-T and ptgs2 Gene Expression

l-Carnitine Supplementation during In Vitro Maturation and In Vitro Culture Does not Affect the Survival Rates after Vitrification and Warming but Alters Inf-T and ptgs2 Gene Expression

L-Carnitine Is Involved in Hyperbaric Oxygen-Mediated Therapeutic Effects in High Fat Diet-Induced Lipid Metabolism Dysfunction

Treatment of mouse cumulus‐oocyte complexes with L‐carnitine during vitrification and in vitro maturation affects maturation and embryonic developmental rate after parthenogenetic activation

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