l-carnitine modulates free mitochondrial DNA DAMPs and platelet storage lesions during storage of platelet concentrates

Bagheri, Saeede; Samiee, Shahram; Zarif, Mahin Nikougoftar; Deyhim, Mohammad Reza

doi:10.1007/s11239-022-02725-2

Cited by 3 publications

(5 citation statements)

References 32 publications

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“…The increase in free mtDNA is consistent with our previous study on PCs stored for 7 days and the results of Chen et al on apheresis PCs during 7 days of storage [15,19]. However, some studies show continuous mtDNA release in the initial days of storage, followed by a decline on the last day, possibly attributed to the degradation of mtDNA during storage [8,16].…”

Section: Discussionsupporting

confidence: 92%

“…Additionally, another investigation conducted by our team revealed elevated reactive oxygen species (ROS) production during the storage of PCs [16,17]. Therefore, we thought it would be valuable to investigate the correlation between platelet activation markers and free mtDNA in PCs during the 7-day storage period.…”

Section: Introductionmentioning

confidence: 99%

“…We have previously shown that adding resveratrol, an antioxidant polyphenol, to PCs reduced free mtDNA and maintained biochemical parameters in PCs, possibly by decreasing platelet activation [15]. Additionally, another investigation conducted by our team revealed elevated reactive oxygen species (ROS) production during the storage of PCs [16, 17]. Therefore, we thought it would be valuable to investigate the correlation between platelet activation markers and free mtDNA in PCs during the 7‐day storage period.…”

Section: Introductionmentioning

confidence: 99%

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A tight interplay between platelet activation and mitochondrial DNA release promotes platelet storage lesion in platelet concentrates

Haeri,

Samiee,

Beigi

et al. 2024

Vox Sanguinis

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Background and ObjectivesPlatelet storage lesion (PSL) adversely affects the quality of platelet concentrates (PCs). Platelets are prone to activation during storage. Moreover, elevated free mitochondrial DNA (mtDNA) levels in PCs are associated with a higher risk of adverse transfusion reactions. Therefore, we aimed to evaluate the correlation between platelet activation markers and mtDNA release during PC storage.Materials and MethodsSix PCs prepared by the platelet‐rich plasma method were assessed for free mtDNA copy number using quantitative real‐time PCR and CD62P (P‐selectin) expression by flow cytometry on days 0 (PC collection day), 3, 5 and 7 of storage. Lactate dehydrogenase (LDH) activity, pH, platelet count, mean platelet volume (MPV) and platelet distribution width (PDW) were measured as well. The correlation between free mtDNA and other PSL parameters, and the correlation between all parameters, was determined.ResultsSignificant increases in free mtDNA, MPV and PDW, and a significant decrease in platelet count and pH were observed. CD62P expression and LDH activity elevated significantly, particularly on storage days 5–7 and 0–3, respectively. Moreover, a moderate positive correlation (r = 0.61) was observed between free mtDNA and CD62P expression. The r values between free mtDNA and LDH, pH, platelet count, MPV and PDW were 0.81, −0.72, −0.49, 0.81 and 0.77, respectively.ConclusionThe interplay between platelet activation and mtDNA release in promoting PSL in PCs may serve as a promising target for future research on applying additive solutions and evaluating the quality of PCs to improve transfusion and clinical outcomes.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A tight interplay between platelet activation and mitochondrial DNA release promotes platelet storage lesion in platelet concentrates

Haeri,

Samiee,

Beigi

et al. 2024

Vox Sanguinis

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“…19 Furthermore, lactate dehydrogenase (LDH) is an enzyme that converts pyruvate to lactate and is released due to membrane damage in cells. 20 Viral infections and lung injuries like pneumonia and COVID-19 can cause LDH levels to increase, and this increase has been associated with the development of COVID-19 disease. 21 The aim of this study was to detect the coronavirus using qPCR and determine the correlation between the Cq level and the results of hematological and inflammatory tests.…”

Section: Introductionmentioning

confidence: 99%

“…It plays a significant role in the activation of cytokines in COVID‐19 disease 19 . Furthermore, lactate dehydrogenase (LDH) is an enzyme that converts pyruvate to lactate and is released due to membrane damage in cells 20 . Viral infections and lung injuries like pneumonia and COVID‐19 can cause LDH levels to increase, and this increase has been associated with the development of COVID‐19 disease 21 …”

Section: Introductionmentioning

confidence: 99%

Cq values as an indicator for COVID‐19 outcomes: A study of the correlation between laboratory parameters

Safdari,

Bagheri,

Talkhi

et al. 2024

Immunity Inflam & Disease

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ObjectiveThe ongoing outbreak of the respiratory disease coronavirus disease 2019 (COVID‐19) is currently presenting a major global health threat. This pandemic is unprecedented in recent human history. The objective of this study was to examine the relationship between cycle quantitation (Cq) and laboratory parameters in COVID‐19 patients, aiming to determine if Cq levels can provide valuable insights into the COVID‐19 disease.MethodsThis study involved 234 participants who were divided into case and control groups. Real‐time PCR tests were used to diagnose COVID‐19 cases in the study participants. Blood tests, including complete blood count, C‐reactive protein (CRP), erythrocyte sedimentation rate (ESR), lactate dehydrogenase (LDH), D‐dimer, IgG, and IgM, were also conducted. Statistical analysis was performed using SPSS 22 software.ResultsThe findings showed that COVID‐19‐positive cases had significantly higher levels of the neutrophil‐to‐lymphocyte ratio (NLR), platelet‐to‐lymphocyte ratio (PLR), D‐dimer, ESR, CRP, and LDH compared to normal cases. Additionally, the case group had significantly lower lymphocyte and platelet counts. There was a statistically significant positive correlation between Cq levels and lymphocyte count (r = .124, p = .014). Conversely, there was a statistically significant inverse correlation between Cq levels and NLR (r = −.208, p = .017). Furthermore, the evaluation of hematological, inflammatory, and biochemical indexes in COVID‐19 patients using the receiver‐operating characteristics curve demonstrated statistically appropriate sensitivity and specificity.ConclusionOur outcomes indicated a significant association between Cq levels and PLR, NLR, D‐dimer, CRP, and ESR in COVID‐19 patients. Consequently, including the report of laboratory parameters alongside Cq values offers a promising prognosis.

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Resveratrol reduces platelet storage lesion by preventing free mitochondrial DNA (mtDNA) accumulation in platelet concentrates during storage

Haeri

Samiee

Hajati

et al. 2023

J Thromb Thrombolysis

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l-carnitine modulates free mitochondrial DNA DAMPs and platelet storage lesions during storage of platelet concentrates

Cited by 3 publications

References 32 publications

A tight interplay between platelet activation and mitochondrial DNA release promotes platelet storage lesion in platelet concentrates

A tight interplay between platelet activation and mitochondrial DNA release promotes platelet storage lesion in platelet concentrates

Cq values as an indicator for COVID‐19 outcomes: A study of the correlation between laboratory parameters

Resveratrol reduces platelet storage lesion by preventing free mitochondrial DNA (mtDNA) accumulation in platelet concentrates during storage

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