L-carnitine ameliorates methotrexate-induced ovarian dysfunction in female rats

Abstract

This study aimed to investigate the effect of L-carnitine (L-car) supplementation on methotrexate (MTX) related ovarian dysfunction. Fifty female albino rats weighing 200 -250 g were randomly divided into five groups (10 rats each); the duration of the experiment was four weeks. Control group received saline (IP). MTX group received 1 mg/kg (IP) once weekly for four weeks. Whereas, MTX+L-car50, MTX+L-car100 and MTX+folic acid groups: daily received either L-car (50 or 100 mg/kg) or folic acid 250 µg/kg respectively (orally) plus 1 mg/kg methotrexate (IP) once weekly for four weeks. Treatment of the rats with MTX produced a significant decrease in body and ovarian weights, ovarian index, serum total antioxidant capacity (TAC) and serum anti-Müllerian hormone (AMH). However, serum total oxidant status (TOS), Oxidative stress index (OSI), ovarian tissue malondialdehyde (MDA), nitric oxide (NO) and caspase-3 levels were significantly increased. Besides, there were histological abnormalities in the ovarian tissues with a significant increase in antral follicles count (AFC). Administration of either L-car (50 or 100 mg/kg) or folic acid corrected the abnormal biochemical parameters and improved the histopathological abnormalities and AFC. These data suggested that L-car might protect against MTX-induced ovarian dysfunction in female rats through antioxidant, anti-apoptotic effects and improving the ovarian reserve.