L-carnitine ameliorates abnormal vulnerability of steatotic rat livers to cold ischemic preservation

Tolba, René; Pütz, Uwe; Decker, D.; Dombrowski, Frank; Lauschke, H.

doi:10.1097/01.tp.0000093832.15249.ad

Cited by 22 publications

(31 citation statements)

References 30 publications

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“…Thus, therapies which are effective in non-steatotic livers may either prove useless in the presence of steatosis or the effective drug dose may differ between the two types of grafts (13,91,92). On the other hand, there may be drugs that would only be effective in steatotic livers (94,95).…”

Section: Steatosismentioning

confidence: 99%

The response of the hepatocyte to ischemia

Massip-Salcedo

Roselló-Catafau

Prieto

et al. 2007

Liver International

117

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Abstract:Background: Ischemia-reperfusion (I/R) injury associated with hepatic resections and liver transplantation remains a serious complication in clinical practice, in spite of several attempts to solve the problem. Aims: To evaluate the response of the hepatocyte to ischemia Methods: Published data are thus revised. Results: The response of the hepatocyte to ischemia is based on the sensitivity of hepatocytes to different types of ischemia, the kind of cell death of the hepatocyte when it is subjected to ischemia, and on the response of the hepatocyte to the different times and extents of ischemia. Clinical factors including starvation, graft, age, and hepatic steatosis, all of which contribute to enhancing liver susceptibility to ischemia/reperfusion injury. Conclusion: Ischemic preconditioning, based on the induction of a brief ischemia to the liver prior to a prolonged ischemia, has been applied in tumor hepatic resections for reducing hepatic I/R injury and recent clinical studies suggest that this surgical strategy could be appropriate for liver transplantation.

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Section: Steatosismentioning

confidence: 99%

The response of the hepatocyte to ischemia

Massip-Salcedo

Roselló-Catafau

Prieto

et al. 2007

Liver International

117

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“…Similarly, at the end of cold ischemia, ATP levels showed no difference between steatotic and lean livers [64]. However, the recovery of ATP levels in postcold PRI steatotic livers was reportedly slower [64], with decreased ATP [44,53,57,61,64,66,71,73,75,78] and increased ADP concentrations [65], and the net effect of decreased overall energy charge [76].…”

Section: Bioenergetics Hepatic Energy Status (Table 6)mentioning

confidence: 99%

“…Glutamate dehydrogenase is a mitocho ndrial enzyme and increases in glutamate dehydrogenase release were used to reflect leakage from damaged or necrotic hepatocytes [67]. Eleven studies reported on glutamate dehydrogenase and in all cases, steatotic livers showed a significant increase in glutamate dehydrogenase levels in the perfusate post-IRI indicating increased mitochondrial damage in steatotic livers [61,62,67,68,[71][72][73][74][75][76][77].…”

Section: Glutamate Dehydrogenase and Superoxidementioning

confidence: 99%

“…QUORUM Diagram. [18]; b, [19]; c, [20]; d, [21]; e, [22]; f, [23], g, [24]; h, [25]; i, [26]; j, [27]; k, [28]; l, [29]; m, [30]; n, [17]; o, [31]; p, [32]; q, [33]; r, [34]; s, [35]; t, [36]; u, [37] [38]; b, [39]; c, [40]; d, [41]; e, [42]; f, [43]; g, [44]; h, [45]; i, [46]; j, [47]; k, [48]; l, [49]; m, [50]; n, [51]; o, [52] [62]; c, [63]; d, [64]; e, [65]; f, [66]; g, [67]; h, [68]; i, [69]; j, [70]; k, [71]; l, [17]; m, [60]; n, [72]; o, [73]<...>…”

Section: Papers Identified From Reference Lists N=19mentioning

confidence: 99%

“…Using different substrate/inhibitor protocols, researchers are now able to test the flux through different respiratory complexes within the ETS and estimate respiration efficiencies [89]. Complex I substrates are derived from tricarboxylic acid cycle dehydrogenase reactions and release reduced nicotinamide adenine dinucleotide, while complex II, which is part of the tricarboxylic acid cycle is directly fueled by [40]; b, [40,64]; c, [27]; d, [64]; e, [40,45,64]; f, [27,40,64]; g, [34]; h, [19]; i, [27,38,40,47,64]; j, [24,27]; k, [23,27]; l, [22,43,64]; m, [22,43,45,64]; n, [27,43,65]; o, [22,30,37,39,41,44,49,50,53,57,61,64,66,…”

Section: Bioenergetics Mitochondrial Function (Table 6)mentioning

confidence: 99%

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Effect of Hepatic Steatosis on Bioenergetic Function During Hepatic Ischemia-reperfusion: A Systematic Review

Chu¹

2013

TOTRANSJ

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Abstract:Background: The impact of hepatic steatosis on bioenergetics following hepatic ischemia-reperfusion injury (IRI) remains controversial and is associated with variable reports on its outcome. Large numbers of studies have been published examining the relationship between hepatic steatosis and cellular bioenergetics following hepatic IRI. This systematic review evaluates these studies.Methods: An electronic search of the Medline and Embase databases (January 1946 to June 2012) was performed to select studies that reported relevant outcomes in animal models or patients with hepatic steatosis subjected to IRI.Results: A total of 489 articles were identified, of which 63 animal studies met the predefined criteria and were included in the study. There was large variation in the type of animal model, duration and type of IRI utilized and histological description of hepatic steatosis. Bioenergetic impairments appear to increase the susceptibility of steatotic livers to IRI. The most common impairment was decreased adenosine triphosphate recovery with increased oxidative stress following IRI. Impaired mitochondrial function play a key role in the susceptibility of steatotic livers to IRI.Conclusions: Animals with >30% hepatic steatosis have been shown to have poor outcome following IRI. Despite limitations of different experimental models and inconsistency in histological description, impaired mitochondrial function and bioenergetics appear to be important mediators in the decreased tolerance of steatotic livers to IRI. Future studies need to be consistent and clinically relevant to further improve our understanding of this issue.

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Preservation of steatotic livers: A comparison between cold storage and machine perfusion preservation

et al. 2007

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Liver grafts are frequently discarded due to steatosis. Steatotic livers can be classified as suboptimal and deteriorate rapidly during hypothermic static preservation, often resulting in graft nonfunction. Hypothermic machine perfusion (MP) has been introduced for preservation of donor livers instead of cold storage (CS), resulting in superior preservation outcomes. The aim of this study was to compare CS and MP for preservation of the steatotic donor rat liver. Liver steatosis was induced in male Wistar rats by a choline-methionine-deficient diet. After 24 hours hypothermic CS using the University of Wisconsin solution (UW) or MP using UW-Gluconate (UW-G), liver damage (liver enzymes, perfusate flow, and hyaluronic acid clearance) and liver function (bile production, ammonia clearance, urea production, oxygen consumption, adenosine triphosphate [ATP] levels) were assessed in an isolated perfused rat liver model. Furthermore, liver biopsies were visualized by hematoxylin and eosin staining. Animals developed 30 to 60% steatosis. Livers preserved by CS sustained significantly more damage as compared to MP. Bile production, ammonia clearance, urea production, oxygen consumption, and ATP levels were significantly higher after MP as compared to CS. These results were confirmed by histology. In conclusion, MP improves preservation results of the steatotic rat liver, as compared to CS. Liver Transpl 13: 497-504, 2007.

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L-carnitine ameliorates abnormal vulnerability of steatotic rat livers to cold ischemic preservation

Abstract: L-carnitine represents a feasible metabolic adjunct for a safe and more successful preservation of ischemia-reperfusion-sensitive steatotic livers.

Cited by 22 publications

References 30 publications

The response of the hepatocyte to ischemia

The response of the hepatocyte to ischemia

Effect of Hepatic Steatosis on Bioenergetic Function During Hepatic Ischemia-reperfusion: A Systematic Review

Preservation of steatotic livers: A comparison between cold storage and machine perfusion preservation

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