L-Asparaginase-an Anti Tumor Agent Production by Fusarium Equiseti Using Solid State Fermentation

Bb, Kaliwal; Hosamani, Rashmi C

doi:10.9735/0975-4423.3.2.88-99

Cited by 35 publications

(8 citation statements)

References 19 publications

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“…Sucrose and fructose also supported l -asparaginase production to a significant degree but glucose acted as good inducer and primary source of carbon for biosynthesis of l -asparaginase using C 7 . Several reports suggest that glucose serves as a best carbon source for l -asparaginase production and a similar effect was observed for l -asparaginase production using Aspergillus and Fusarium strains (Baskar and Renganathan 2012; Hosamani and Kaliwal 2011). Effect of nitrogen compounds on l -asparaginase by C 7 was studied by supplementing nitrogen sources (asparagine, yeast extract, peptone and sodium nitrate) to MCD medium.…”

Section: Resultsmentioning

confidence: 53%

Isolation and screening of l-asparaginase free of glutaminase and urease from fungal sp.

Doriya

Kumar

2016

3 Biotech

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l-Asparaginase is a chemotherapeutic drug used in the treatment of acute lymphoblastic leukaemia (ALL), a malignant disorder in children. l-Asparaginase helps in removing acrylamide found in fried and baked foods that is carcinogenic in nature. l-Asparaginase is present in plants, animals and microbes. Various microorganisms such as bacteria, yeast and fungi are generally used for the production of l-asparaginase as it is difficult to obtain the same from plants and animals. l-Asparaginase from bacteria causes anaphylaxis and other abnormal sensitive reactions due to low specificity to asparagine. Toxicity and repression caused by bacterial l-asparaginase shifted focus to eukaryotic microorganisms such as fungi to improve the efficacy of l-asparaginase. Clinically available l-asparaginase has glutaminase and urease that may lead to side effects during treatment of ALL. Current work tested 45 fungal strains isolated from soil and agricultural residues. Isolated fungi were tested using conventional plate assay method with two indicator dyes, phenol red and bromothymol blue (BTB), and results were compared. l-Asparaginase activity was measured by cultivating in modified Czapek–Dox medium. Four strains have shown positive result for l-asparaginase production with no urease or glutaminase activity, among these C7 has high enzyme index of 1.57 and l-asparaginase activity of 33.59 U/mL. l-Asparaginase production by C7 was higher with glucose as carbon source and asparagine as nitrogen source. This is the first report focussing on fungi that can synthesize l-asparaginase of the desired specificity. Since the clinical toxicity of l-asparaginase is attributed to glutaminase and urease activity, available evidence indicates variants negative for glutaminase and urease would provide higher therapeutic index than variants positive for glutaminase and urease.

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Section: Resultsmentioning

confidence: 53%

Isolation and screening of l-asparaginase free of glutaminase and urease from fungal sp.

Doriya

Kumar

2016

3 Biotech

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“…1 ). Previous studies have shown that a nitrogen source is the limiting factor and plays key role in the L-asparaginase production [29]. It seems that L-asparagine might be an inducer for L-asparaginase production and hence the production of the enzyme is more feasible at the presence of this nitrogen source compared to L-arginine and NaNO 3 + (NH 4 ) 2 SO 4 .…”

Section: Resultsmentioning

confidence: 99%

Purification, Characterization and Comparison between Two New L-asparaginases from PG03 and PG04

Rahimzadeh¹,

Poodat²,

Javadpour³

et al. 2016

Open Biochem J.

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Background:L-asparaginase has been used as a chemotherapeutic agent in treatment of lymphoblastic leukemia. In the present investigation, Bacillus sp. PG03 and Bacillus sp. PG04 were studied.Methods:L- asparaginases were produced using different culture media and were purified using ion exchange chromatography.Results:Maximum productivity was obtained when asparagine was used as the nitrogen source at pH 7 and 48 h after cultivation. New intracellular L-asparaginases showed an apparent molecular weight of 25 kDa and 30 kDa by SDS-PAGE respectively. These enzymes were active in a wide pH range (3-9) with maximum activity at pH 6 for Bacillus PG03 and pH 7 for Bacillus PG04 L-asparaginase. Bacillus PG03 enzyme was optimally active at 37 ˚C and Bacillus PG04 maximum activity was observed at 40˚C. Kinetic parameters km and Vmax of both enzymes were studied using L-asparagine as the substrate. Thermal inactivation studies of Bacillus PG03 and Bacillus PG04 L-asparaginase exhibited t1/2 of 69.3 min and 34.6 min in 37 ˚C respectively. Also T50 and ∆G of inactivation were measured for both enzymes.Conclusion: The results revealed that both enzymes had appropriate characteristics and thus could be a potential candidate for medical applications.

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“…3a, 10% moisture is insufficient for enzyme production, while at higher levels of moisture content, the enzyme activity was reduced. In SSF using agricultural residues, the optimum moisture content for L-asparaginase production by non-endophytic fungi lies in the range of 50-70% [9][10][11] . Reduction in L-asparaginase activity at low moisture level has also been reported in case of Penicillium sp.…”

Section: Solid-state Fermentation Using Puf: Effect Of Process Paramementioning

confidence: 99%

“…Reduction in L-asparaginase activity at low moisture level has also been reported in case of Penicillium sp. under SSF with sugarcane bagasse 9 . The amount of moisture content has to be appropriately chosen to permit accessibility to the nutrients in the media.…”

Section: Solid-state Fermentation Using Puf: Effect Of Process Paramementioning

confidence: 99%

“…L-asparaginase is largely produced by Submerged fermentation (SmF). Solid-state fermentation (SSF) for L-asparaginase production by fungi has mainly involved the usage of agricultural wastes [9][10][11] . However, SSF processes utilizing agricultural by-products or non-inert raw materials have disadvantages such as change in the medium characteristics and support degradation over time, difficulties in downstream processing of the products 12 and in biomass estimation 13 .…”

Section: Introductionmentioning

confidence: 99%

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L-Asparaginase Production using Solid-state Fermentation by an Endophytic Talaromyces pinophilus Isolated from Rhizomes of Curcuma amada

Krishnapura¹,

Belur²

2020

J. Pure Appl. Microbiol.

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In recent times, exploration of endophytes for L-asparaginase production is gradually gaining momentum. This work deals with studies on the production of L-asparaginase from Talaromyces pinophilus, an endophytic fungus isolated from the rhizomes of Curcuma amada. L-asparaginase production was carried out by Submerged Fermentation (SmF) followed by Solid-state Fermentation (SSF). A liquid medium was designed and optimized using Plackett-Burman Design and Response Surface Methodology (RSM), under SmF. Additionally, optimal concentrations of various metal salts were incorporated in the optimized liquid medium, by one-factor-at-a-time experiments. To further enhance L-asparaginase production, SSF was carried out using Polyurethane Foam (PUF) as inert support impregnated with the optimized liquid medium. Effects of PUF cube volume, mass of PUF, moisture content, initial medium pH, and incubation temperature on the enzyme production in SSF were optimized by one-factor-at-a-time approach.L-asparaginase production enhanced from 80.8 U/mL in the unoptimized medium to 94.4 U/mL in the optimized medium under SmF. Enzyme production further increased to 120.3 U/mL under SSF by using PUF soaked in the optimized liquid medium. This study highlights the benefits of carrying out SSF with PUF, using the same liquid medium optimized for SmF-a novel approach to enhance the enzyme yield (in our case an increase of about 27% was observed). To the best of our knowledge, this is the first report on the production of L-asparaginase by both SmF and SSF, from an endophyte Talaromyces pinophilus isolated from the rhizomes of Curcuma amada.

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L-Asparaginase-an Anti Tumor Agent Production by Fusarium Equiseti Using Solid State Fermentation

Cited by 35 publications

References 19 publications

Isolation and screening of l-asparaginase free of glutaminase and urease from fungal sp.

Isolation and screening of l-asparaginase free of glutaminase and urease from fungal sp.

Purification, Characterization and Comparison between Two New L-asparaginases from PG03 and PG04

L-Asparaginase Production using Solid-state Fermentation by an Endophytic Talaromyces pinophilus Isolated from Rhizomes of Curcuma amada

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